The construction of two recombinant expression vectors of interleukin-1 gene from Acanthopagrus latus

A pair of primers were designed and synthesized according to the interleukin-1 (IL-1) gene sequence (GenBank accession number is AY669059) of the yellowfin seabream Acanthopgrus latus (Houttuyn). The plasmid pMD18T-IL1 was constructed after amplifying open reading frame cDNA sequence of the IL-1 gene by RT-PCR. The sequence of propeptide and predicted mature peptide were linked into expression vector pQE30 and two recombinant plasmids pQE30-pIL1 and pQE30-mIL1 were constructed. Restriction analysis, PCR amplification and sequencing demonstrated that the target fragments were inserted into pQE30 correctly.