Citation: | LI Jian-zhu, JIANG Shi-gui. The construction of two recombinant expression vectors of interleukin-1β gene from Acanthopagrus latus[J]. South China Fisheries Science, 2005, 1(3): 37-41. |
A pair of primers were designed and synthesized according to the interleukin-1β (IL-1β) gene sequence (GenBank accession number is AY669059) of the yellowfin seabream Acanthopgrus latus (Houttuyn). The plasmid pMD18T-IL1β was constructed after amplifying open reading frame cDNA sequence of the IL-1β gene by RT-PCR. The sequence of pro-peptide and predicted mature peptide were linked into expression vector pQE30 and two recombinant plasmids pQE30-pIL1β and pQE30-mIL1β were constructed. Restriction analysis, PCR amplification and sequencing demonstrated that the target fragments were inserted into pQE30 correctly.
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