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LI Jian-zhu, JIANG Shi-gui. The construction of two recombinant expression vectors of interleukin-1β gene from Acanthopagrus latus[J]. South China Fisheries Science, 2005, 1(3): 37-41.
Citation: LI Jian-zhu, JIANG Shi-gui. The construction of two recombinant expression vectors of interleukin-1β gene from Acanthopagrus latus[J]. South China Fisheries Science, 2005, 1(3): 37-41.
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The construction of two recombinant expression vectors of interleukin-1β gene from Acanthopagrus latus

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Shanghai Fisheries University, Shanghai 200090, China

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  • Received Date: May 08, 2005
    Graphical Abstract
    • Abstract

  • A pair of primers were designed and synthesized according to the interleukin-1β (IL-1β) gene sequence (GenBank accession number is AY669059) of the yellowfin seabream Acanthopgrus latus (Houttuyn). The plasmid pMD18T-IL1β was constructed after amplifying open reading frame cDNA sequence of the IL-1β gene by RT-PCR. The sequence of pro-peptide and predicted mature peptide were linked into expression vector pQE30 and two recombinant plasmids pQE30-pIL1β and pQE30-mIL1β were constructed. Restriction analysis, PCR amplification and sequencing demonstrated that the target fragments were inserted into pQE30 correctly.

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    • References (14)
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