Volume 4 Issue 2
Apr.  2008
Turn off MathJax
Article Contents
Abstract
References
Related Articles
QU Youjun, WU Yong, LI Jiaer, LIU Chuwu. RAPD analysis of genetic difference among five species of grouper[J]. South China Fisheries Science, 2008, 4(2): 56-62.
Citation: QU Youjun, WU Yong, LI Jiaer, LIU Chuwu. RAPD analysis of genetic difference among five species of grouper[J]. South China Fisheries Science, 2008, 4(2): 56-62.
shu

RAPD analysis of genetic difference among five species of grouper

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China

More Information
  • Received Date: December 16, 2007
  • Revised Date: December 28, 2007
    Graphical Abstract
    • Abstract

  • Genetic diversity and molecular evolution of five species of grouper, viz.Epinephelus lanceolatus, Cromileptes altivelis, E.fuscoguttatus, E.coioides and E.fario, were detected using RAPD technique. A total 120 random primers were evaluated for the usefulness. And thereinto, 19 random primers were selected according to the effect of PCR of genomic DNA of 5 species of grouper. The results showed as the followings: the polymorphic loci ratio in E.lanceolatus, C.altivelis, E.fuscoguttatus, E.coioides and E.fario (P) were 64.23%, 72.61%, 60.34%, 69.97% and 73.94%, respectively. The mean genetic similarity index of intra-species (S) were 0.8238, 0.8110, 0.8345, 0.8277, 0.8064, respectively, the mean genetic distances of intra-species (D) were 0.1762, 0.1890, 0.1655, 0.1723 and 0.1936, respectively, Nei′s gene diversity (H) were 0.1189, 0.1364, 0.1028, 0.1439 and 0.1648, respectively, and Shannon′s information index (Hi) were 0.1801, 0.1992, 0.1530, 0.2100, 0.2434, respectively. The mean genetic distances of inter-species (Dxy) were between 0.3964~0.6085.

    • FullText(HTML)
    • References (18)
  • [1]
    黄原. 分子系统学——原理、方法及应用[M]. 北京: 中国农业出版社, 1998: 186-193. https://book.douban.com/subject/4773037/
    [2]
    LYNCH M. The similarity index and DNA fingerprinting[J]. Mol Biol Evol, 1990(7): 478-484. doi: 10.1093/oxfordjournals.molbev.a040620
    [3]
    ARMSTRON G J S, GIBBS A J, PEAKALL R, et al. The RAPDistance Package[P/OL]. [1994]. http://www.anu.edu.au/BoZo/software/.
    [4]
    NEI M, LI W H. Mathematical model for studying genetic variation in terms of restriction endonucleases[J]. Proc Nat Acad Sci USA, 1979, 76(10): 5 269-5 273. doi: 10.1073/pnas.76.10.5269
    [5]
    NEI M. Estimation of average heterozygosity and genetic distance from a small number of individuals[J]. Genetics, 1978, 89: 583-590. doi: 10.1093/genetics/89.3.583
    [6]
    CHALMERS K J, WAUGH R, SPRENT J I, et al. Detection of genetic variation between and within populations of Gliricidia sepium and G. maculata using RAPD markers[J]. Heredity, 1992, 69: 465-472. doi: 10.1038/hdy.1992.151
    [7]
    NEI M. Genetic distance between populations[J]. Am Nat, 1972, 106: 283-292. doi: 10.1086/282771
    [8]
    郑莲, 刘楚吾. 4种石斑鱼亲缘关系的RAPD分析[J]. 水产科学, 2004, 23(11): 16-20. doi: 10.3969/j.issn.1003-1111.2004.11.006
    [9]
    尹绍武, 黄海, 廖经球, 等. 四种石斑鱼的分子遗传多样性和亲缘关系的RAPD分析[J]. 海洋学报, 2006, 28(6): 119-126. http://qikan.cqvip.com/Qikan/Article/Detail?id=23511686
    [10]
    GOVINDARAJU G S, JAYASANKAR P. Taxonomic relationship among seven species of grouper (Genus Epinephelus; Family Serranidae) as revealed by RAPD fingerprinting[J]. Mar Bio, 2004, 6(3): 229-237. doi: 10.1007/s10126-003-0021-9
    [11]
    刘楚吾, 刘丽, 王中铎, 等. 军曹鱼的分子遗传特性研究[J]. 热带海洋学报, 2005, 24(1): 77-85. doi: 10.3969/j.issn.1009-5470.2005.01.011
    [12]
    丁少雄, 苏永全, 王军, 等. 闽粤沿海状黄姑鱼野生种群和人工繁育群体遗传多样性的RAPD分析[J]. 海洋科学, 2003, 27(8): 63-66. doi: 10.3969/j.issn.1000-3096.2003.08.017
    [13]
    中国科学院动物研究所, 中国科学院海洋研究所, 上海水产学院. 南海鱼类志[M]. 北京: 科学出版社, 1962: 292-308. https://book.douban.com/subject/10512105/
    [14]
    孟庆闻, 苏锦祥, 缪学祖, 等. 鱼类分类学[M]. 北京: 中国农业出版社, 1995: 606-610;620-622. https://book.douban.com/subject/1059981/
    [15]
    成庆泰, 郑葆珊. 中国鱼类系统检索(上、下册)[M]. 北京: 科学出版社: 287-293;1 035-1 047. https://book.douban.com/subject/6872030/
    [16]
    夏德全, 曹莹, 吴婷婷, 等. 用RAPD方法分析太湖大银鱼、太湖新银鱼和寡齿新银鱼的亲缘关系[J]. 中国水产科学, 2000, 7(1): 12-15. doi: 10.3321/j.issn:1005-8737.2000.01.003
    [17]
    张四明, 邓怀, 汪登强, 等. 7种鲟形目鱼类亲缘关系的随机扩增多态性DNA研究[J]. 自然科学进展, 1999, 9(9): 818-823. doi: 10.1088/0256-307X/15/12/024
    [18]
    邹曙明, 楼允东. 用RAPD方法研究草鱼、柏氏鲤和3个地理种群鲤的亲缘关系[J]. 中国水产科学, 2000, 7(1): 6-11. doi: 10.3321/j.issn:1005-8737.2000.01.002
    • Related Articles

  • Related Articles

    [1]XIAO Bo, ZHOU Shengjie, WANG Yinggang, FU Zhengyi, FANG Wei, YU Gang, MA Zhenhua. Effects of fermented Astragalus membranaceus on growth, digestion, immune function and ammonia nitrogen resistance of Epinephelus fuscoguttatus[J]. South China Fisheries Science, 2023, 19(2): 161-169. DOI: 10.12131/20220197
    [2]LI Zhentong, TIAN Yongsheng, CHENG Meiling, WU Yuping, ZHANG Jingjing, LI Ziqi, WANG Linna, MA Wenhui, PANG Zunfang, SUN Lijuan, ZHAI Jieming. Comparison of development and growth of hybrid Chromileptes altivelis (♀) × Epinephelus tukula (♂)[J]. South China Fisheries Science, 2020, 16(1): 17-26. DOI: 10.12131/20190168
    [3]XIE Shiwei, TIAN Lixia, LIU Yongjian, ZHANG Haitao, NIU Jin, LIANG Jianjun, FANG Weiping, SU Jicai. Vitamin D3 requirement of grouper (Epinephelus coioides) in practical diet[J]. South China Fisheries Science, 2019, 15(4): 61-67. DOI: 10.12131/20190029
    [4]WU Shuiqing, ZHENG Leyun, LUO Huiyu, HUANG Zhongchi, LIN Kebing, QIU Fengyan, WU Jingling, LIN Jinbo. Comparison of morphological traits of hybrid groupers (Epinephelus coioides♀×E.akaara♂) and their parents[J]. South China Fisheries Science, 2017, 13(5): 47-54. DOI: 10.3969/j.issn.2095-0780.2017.05.007
    [5]ZHAO Shuyan, LIN Heizhao, HUANG Zhong, ZHOU Chuanpeng, WANG Jun, WANG Yun, QI Changle. Effect of dietary protein level on growth performance, plasma biochemical indices and flesh quality of grouper (Epinephelus lanceolatus×E.fuscoguttatus) at two growth stages[J]. South China Fisheries Science, 2017, 13(4): 87-96. DOI: 10.3969/j.issn.2095-0780.2017.04.011
    [6]PENG Chao, MA Hongling, GUO Zhixun, FENG Juan, SU Youlu, WEN Weigeng, QIU Lihua. Tissue distribution of grouper iridovirus genus Ranavirus in infected farmed juvenile giant grouper (Epinephelus lanceolatus)[J]. South China Fisheries Science, 2015, 11(3): 88-94. DOI: 10.3969/j.issn.2095-0780.2015.03.014
    [7]LI Jia'er, WU Shuiqing, OU Youjun, LIU Jianghua, WEN Jiufu, WANG Pengfei. Study on mucous and gastrin cells of digestive tract in Qinglong grouper (Epinephelus coioides♀×E.lanceolatus♂) juveniles[J]. South China Fisheries Science, 2015, 11(3): 67-73. DOI: 10.3969/j.issn.2095-0780.2015.03.011
    [8]CHEN Xinghan, GUO Liang, LI Mingming, MENG Zining, LIN Haoran. Analysis of RAPD and mitochondrial Cyt b gene sequences of three cultured stocks of Epinephelus coioides from Guangdong Province[J]. South China Fisheries Science, 2014, 10(4): 27-33. DOI: 10.3969/j.issn.2095-0780.2014.04.005
    [9]CHEN Dawei, DENG Li, LIU Zhigang, MENG Boyuan, MA Yijian. Cloning of antimicrobial peptide hepcidin from Epinephelus coioides and prokaryotic fusion expression of its predicted mature peptide[J]. South China Fisheries Science, 2011, 7(1): 1-7. DOI: 10.3969/j.issn.2095-0780.2011.01.001
    [10]LIN Heizhao, LIU Yongjian, HE Jianguo, ZHENG Wenhui, LI Zhuojia, TIAN Lixia. Effects of starvation on fatty acid composition of muscle and liver in grouper, Epinephelus coioides[J]. South China Fisheries Science, 2006, 2(4): 1-6.

Catalog

    Get Citation
    PDF
    XML
    Article views (5756) PDF downloads (3768) Cited by()
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return