| Citation: |
CHEN Dawei, DENG Li, LIU Zhigang, MENG Boyuan, MA Yijian. Cloning of antimicrobial peptide hepcidin from Epinephelus coioides and prokaryotic fusion expression of its predicted mature peptide[J]. South China Fisheries Science, 2011, 7(1): 1-7. DOI: 10.3969/j.issn.2095-0780.2011.01.001
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Based on the known hepcidin cDNA sequences in teleosts, we designed some degenerate primers and cloned a 246 bp cDNA sequence with complete open reading frame from the liver of Epinephelus coioides, an important commercial marine fish.According to Blast analysis, the obtained sequence, named asE.coioideshepcidin-like antimicrobial peptide precursor, is a member of fish hepcidin gene family. The deduced amino acid sequence has such features as follows: 1) the similarity of hepcidins between the present signal peptide and that of most other fish is 67%~87%; 2) twenty animo acids in the C-terminal region contain 8 cysteines, which is the typical feature of conserved sequence of mature peptide in most animals′ hepcidins; 3) [RX(K/R)R] motif for propeptide convertases of the reported hepcidins is not found; 4) the similarity of the deduced amino acids between the present sequence and other 2 hepcidin sequences of E.coioides (GU391241 and GU391242) registered in GenBank is 36% and 33%, respectively. Phylogenetic neighbor-joining tree indicates that the deduced amino acid sequences of above 3 hepcidins of E.coioides do not cluster into one clade. Thus, the hepcidin obtained in the present study is presumably a new isoform of hepcidin gene family in E.coioides. The prokaryotic fusion expression vector of its predicted mature hepcidin peptide has been constructed and expressed successfully in Escherichia coli BL21 (DE3), which lays foundation for future study.
| [1] |
唐啸尘, 刘晓春, 林浩然. 斜带石斑鱼仔鱼变态过程中甲状腺的发育变化[J]. 水生生物学报, 2010, 34(1):210-214. doi: 10.3724/SP.J.1035.2010.00210
TANG Xiaochen, LIU Xiaochun, LIN Haoran. The development of thyroid of orange-spotted grouper (Epinephelus coioides)larvae during metamorphosis[J]. Acta Hydrobiologica Sinica, 2010, 34(1):210-214. (in Chinese) doi: 10.3724/SP.J.1035.2010.00210
|
| [2] |
龚艳清, 陈信忠, 王军, 等. 福建南部养殖石斑鱼暴发性疾病流行调查[J]. 福建农林大学学报, 2006, 35(5):532-537. doi: 10.3321/j.issn:1671-5470.2006.05.019
GONG Yanqing, CHEN Xinzhong, WANG Jun, et al. Investigation on the epidemic of fulminant infectious disease of cultured rockfish in the south Fujian[J]. J Fujian Agric Forestry Univ, 2006, 35(5):532-537. (in Chinese) doi: 10.3321/j.issn:1671-5470.2006.05.019
|
| [3] |
聂品. 鱼类非特异性免疫研究的新进展[J]. 水产学报, 1997, 21(1):69-72. https://cstj.cqvip.com/Qikan/Article/Detail?id=2455608&from=Qikan_Article_Detail
NIE Pin. Recent advances of non-specific immunity in fish[J]. J Fish China, 1997, 21(1):69-72. (in Chinese) https://cstj.cqvip.com/Qikan/Article/Detail?id=2455608&from=Qikan_Article_Detail
|
| [4] |
叶星, 白俊杰. 抗菌肽的研究及其在水产上的应用前景[J]. 大连水产学院学报, 2000, 15(4):274-279. doi: 10.3969/j.issn.1000-9957.2000.04.008
YE Xing, BAI Junjie. Researches on anti-microbial peptides and its application perspective in aquaculture[J]. J Dalian Fish Univ, 2000, 15(4):274-279. (in Chinese) doi: 10.3969/j.issn.1000-9957.2000.04.008
|
| [5] |
HUNTER H N, FULTON D B, GANZ T, et al. The solution structure of human hepcidin, a peptide hormone with antimicrobial activity that is involved in iron uptake and hereditary hemochromatosis[J]. J Biol Chem, 2002, 277(40):37597-37603. doi: 10.1074/jbc.M205305200
|
| [6] |
SHIKE H, LAUTH X, WESTERMAN M E, et al. Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge[J]. Eur J Biochem, 2002, 269(8):2232-2237. doi: 10.1046/j.1432-1033.2002.02881.x
|
| [7] |
SOW F B, FLORENCE W C, SATOSKAR A R, et al. Expression and localization of hepcidin in macrophages: a role in host defense against tuberculosis[J]. J Leukoc Biol, 2007, 82(4):934-945. doi: 10.1189/jlb.0407216
|
| [8] |
HILTON K B, LAMBERT L A. Molecular evolution and characterization of hepcidin gene products in vertebrates[J]. Gene, 2008, 415(1/2):40-48. doi: 10.1016/j.gene.2008.02.016
|
| [9] |
WANG Y D, KUNG C W, CHI S C, et al. Inactivation of nervous necrosis virus infecting grouper (Epinephelus coioides) by epinecidin-1 and hepcidin 1-5 antimicrobial peptides, and downregulation of Mx2 and Mx3 gene expressions[J]. Fish Shellfish Immunol, 2010, 28(1):113-120. doi: 10.1016/j.fsi.2009.10.001
|
| [10] |
杨明, 王克坚, 曲海, 等. 真鲷鳃组织cDNA文库的构建与hepcidin抗菌肽基因序列的扩增[J]. 水产学报, 2006, 30(5):627-632. doi: 10.3321/j.issn:1000-0615.2006.05.008
YANG Ming, WANG Kejian, QU Haidong, et al. Construction of cDNA library of gills from Pagrus major with the cloning of hepcidin[J].J Fish China, 2006, 30(5):627-632. (in Chinese) doi: 10.3321/j.issn:1000-0615.2006.05.008
|
| [11] |
HUANG P H, CHEN J Y, KUO C M. Three different hepcidins from tilapia, Oreochromis mossambicus: analysis of their expressions and biological functions[J]. Mol Immunol, 2007, 44(8):1922-1934. doi: 10.1016/j.molimm.2006.09.031
|
| [12] |
白俊杰, 刘碧莲, 劳海华, 等. 大口黑鲈抗菌肽hepcidin cDNA序列和结构分析[J]. 广东海洋大学学报, 2008, 28(1):1-5. doi: 10.3969/j.issn.1673-9159.2008.01.001
BAI Junjie, LIU Bilian, LAO Haihua, et al. Cloning and sequence analysis of cDNA encoding hepcidin from largemouth bass, Microptorus salmonoidesi[J].J Guandong Ocean Univ, 2008, 28(1):1-5. (in Chinese) doi: 10.3969/j.issn.1673-9159.2008.01.001
|
| [13] |
YANG M, WANG K J, CHEN J H, et al. Genomic organization and tissue-specific expression analysis of hepcidin-like genes from black porgy (Acanthopagrus schlegelii B)[J]. Fish Shellfish Immunol, 2007, 23(5):1060-1071. doi: 10.1016/j.fsi.2007.04.011
|
| [14] |
MARTIN-ANTONIO B, JIMENEZ-CANTIZANO R M, SALAS-LEITON E, et al. Genomic characterization and gene expression analysis of four hepcidin genes in the redbanded seabream (Pagrus auriga)[J]. Fish Shellfish Immunol, 2009, 26(3):483-491. doi: 10.1016/j.fsi.2009.01.012
|
| [15] |
李伟, 陈松林. 牙鲆抗菌肽hepcidin基因在大肠杆菌中的融合表达[J]. 高技术通讯, 2007, 17(7):765-770. doi: 10.3321/j.issn:1002-0470.2007.07.020
LI Wei, CHEN Songlin. Fusion expression of hepcidin gene from Paralichthys olivaceus in Escherichia Coli[J]. High Tech Lett, 2007, 17(7):765-770. (in Chinese) doi: 10.3321/j.issn:1002-0470.2007.07.020
|
| [16] |
陈吉刚, 杨季芳, 任萍, 等. 鲈鱼hepcidin 原核表达及生物学活性测定[J]. 水生生物学报, 2010, 34(3): 554-561. doi: 10.3724/SP.J.1035.2010.00554
CHEN Jigang, YANG Jifang, REN Ping, et al. Prokaryotic expression and function analysis of Lateolabrax japonica hepcidin[J]. Acta Hydrobiologica Sinica, 2010, 34(3):554-561. (in Chinese) doi: 10.3724/SP.J.1035.2010.00554
|
| [17] |
王炜, 郑学礼, 陈晓光. 应用伴侣分子在大肠杆菌表达系统高效表达家蝇天蚕素[J]. 热带医学杂志, 2009, 9(2):128-139.
WANG Wei, ZHENG Xueli, CHEN Xiaoguang. High-level expression of Musca domestica cecropin with molecular partner in Escherichia coli[J].J Tropical Med, 2009, 9(2):128-139. (in Chinese)
|
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