Volume 14 Issue 1
Feb.  2018
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RONG Jing, QIU Chaoying, HU Xiao, YANG Xianqing, LI Laihao. Functional properties of glycosylated myofibrillar proteins from purple back flying squids (Symplectoteuthis oualaniensis)[J]. South China Fisheries Science, 2018, 14(1): 68-76. DOI: 10.3969/j.issn.2095-0780.2018.01.009
Citation: RONG Jing, QIU Chaoying, HU Xiao, YANG Xianqing, LI Laihao. Functional properties of glycosylated myofibrillar proteins from purple back flying squids (Symplectoteuthis oualaniensis)[J]. South China Fisheries Science, 2018, 14(1): 68-76. DOI: 10.3969/j.issn.2095-0780.2018.01.009
shu

Functional properties of glycosylated myofibrillar proteins from purple back flying squids (Symplectoteuthis oualaniensis)

  • 1.

    College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

  • 2.

    Key Laboratory of Aquatic Product Processing, Ministry of Agriculture; National R&D  Center for Aquatic Product Processing; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

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  • Received Date: June 06, 2017
  • Revised Date: August 20, 2017
  • Available Online: January 07, 2019
    Graphical Abstract
    • Abstract
  • The myofibrillar proteins extracted from purple back flying squids (Symplectoteuthis oualaniensis) were glycosylated with dextran, alginate oligosaccharide, glucose and lactose. The protein solubility, structure, emulsifying and foaming properties were evaluated. The results show that after saccharides mixing with myofibrillar by 1∶1 (w/w) for 12 h, the SDS-PAGE bands of protein exhibited greater molecular weight, and low molecular saccharides reacted with myofibrillar proteins faster. The protein solubility was improved but the surface hydrophobicity decreased. The secondary structure showed higher β-turn but lower β-sheets content after reaction. Glycosylation did not improve thermal stability of protein effectively. The foaming property and foam stability of glycosylated products were improved while the emulsifying ability and emusion stability decreased. The emulsifying and foaming ability and stability were better in higher ionic solution than in lower ionic solution. The solubility and foaming ability of protein in low ionic solution can be improved effectively, which is meaningful for the application of myofibrillar protein from squids.
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  • [1]
    吴静, 胡晓, 杨贤庆, 等. 鸢乌贼酶解产物的抗氧化稳定性与功能特性[J]. 南方水产科学, 2016, 12(5): 105-111.
    [2]
    于刚, 张洪杰, 杨少玲, 等. 南海鸢乌贼营养成分分析与评价[J]. 食品工业科技, 2014, 35(18): 358-361, 372.
    [3]
    罗永康, 张爱荣. 糖基化反应改善蛋白质功能特性的研究进展[J]. 食品科技, 2004(7): 4-6, 10.
    [4]
    胡爱军, 卢秀丽, 郑捷, 等. 不同频率超声对鲢鱼肌原纤维蛋白结构的影响[J]. 现代食品科技, 2014, 30(3): 23-27.
    [5]
    刘书来, 周欢, 何小庆, 等. 限制性酶解改性鲢鱼肌原纤维蛋白功能性质的研究[J]. 核农学报, 2017, 31(1): 110-117.
    [6]
    SUN Y X, HAYAKAWA S, PUANGMANEE S, et al. Chemical properties and antioxidative activity of glycated alpha-lactalbumin with a rare sugar, D-allose, by Maillard reaction[J]. Food Chem, 2006, 95(3): 509-517.
    [7]
    GROUBET R, CHOBERT J M, HAERTLE T. Functional properties of milk proteins glycated in mild conditions[J]. Sci Aliment, 1999, 19(3/4): 423-438.
    [8]
    黄友如, 华欲飞. 大豆分离蛋白化学改性及其对功能性质影响[J]. 粮食与油脂, 2003(4): 17-19.
    [9]
    王兰, 李新和. 美拉德反应在改进蛋白质功能性质方面的研究进展[J]. 郑州粮食学院学报, 1999, 20(4): 33.
    [10]
    KATO A, SASAKI Y, FURUTA R, et al. Functional protein-polysaccharide conjugate prepared by controlled dry-heating of ovalbumin-dextran mixtures[J]. Agric Biol Chem, 1990, 54(1): 107-112.
    [11]
    SAEKI H. Preparation of neoglycoprotein from carp myofibrillar protein by maillard reaction with glucose: biochemical properties and emulsifying properties[J]. J Agric Food Chem, 1997, 45(3): 680-684.
    [12]
    TAKEDA H, IIDA T, OKADA A, et al. Feasibility study on water solubilization of spawned out salmon meat by conjugation with alginate oligosaccharide[J]. Fish Sci, 2007, 73(4): 924-930.
    [13]
    尤娟, 张佳, 罗永康, 等. 鲢鱼肌原纤维蛋白-低聚异麦芽糖的制备及功能特性评价[J]. 肉类研究, 2009, 23(9): 14-18.
    [14]
    SAEKI H, INOUE K. Improved solubility of carp myofibrillar proteins in low ionic strength medium by glycosylation[J]. J Agric Food Chem, 1997, 45(9): 3419-3422.
    [15]
    SATO R, SAWABE T, KISHIMURA H, et al. Preparation of neoglycoprotein from carp myofibrillar protein and alginate oligosaccharide: improved solubility in low ionic strength medium[J]. J Agric Food Chem, 2000, 48(1): 17-21.
    [16]
    SATO R K S, SAEKI H E A. Stability and emulsion-forming ability of water-soluble fish myofibrillar protein prepared by conjugation with alginate oligosaccharide[J]. J Agric Food Chem, 2003, 51(15): 4376-4381.
    [17]
    李学鹏, 王祺, 周凯, 等. 丙烯醛氧化对大黄鱼肌肉组织和肌原纤维蛋白结构性质的影响[J]. 现代食品科技, 2014, 30(10): 1-7.
    [18]
    MAITENA U, KATAYAMA S, SATO R, et al. Improved solubility and stability of carp myosin by conjugation with albinate oligosaccharide[J]. Fish Sci, 2004, 70(5): 896-902.
    [19]
    管军军, 裘爱泳, 刘晓亚, 等. 微波辐射合成大豆分离蛋白——糖接枝物的功能性[J]. 食品科学, 2005, 26(11): 35-39.
    [20]
    XUE F, LI C, ZHU X W, et al. Comparative studies on the physicochemical properties of soy protein isolate-maltodex-trin and soy protein isolate-gun acacia conjugate prepared through Maillard reaction[J]. Food Res Int, 2013, 51(2): 490-495.
    [21]
    TIAN S J, CHEN J, SMALL D M. Enhancement of solubility and emulsifying properties of soy protein isolates by glucose conjugation[J]. J Food Process Pres, 2011, 35(1): 80-95.
    [22]
    NAKAMURA S, KOBAYASHI K, KATO A. Role of positive charge of lysozyme in the excellent emulsifying properties of maillard-type lysozyme polysaccharide conjugate[J]. J Agric Food Chem, 1994, 42(12): 2688-2691.
    [23]
    SAIGUSA M, NISHIZAWA M, SHIMIZU Y, et al. In vitro and in vivo anti-inflammatory activity of digested peptides derived from salmon myofibrillar protein conjugated with a small quantity of alginate oligosaccharide[J]. Biosci Biot Biochem, 2015, 79(9): 1518-1527.
    [24]
    WU P, SIESLE R W. Two-dimensional correlation analysis of variable-temperature Fourier-transform mid- and near-infrared spectra of polyamide 11[J]. J Mol Struct, 2000, 521(1/2/3): 37-47.
    [25]
    林伟静. 糖基化改性对花生蛋白膜性能的影响及其作用机理研究[D]. 北京: 中国农业科学院, 2015.
    [26]
    OMANA D. Alkali-aided protein extraction from chicken dark meat: chemical and functional properties of recovered proteins[J]. Process Biochem, 2010, 45(3): 375-381.
    [27]
    SANTE-LHOUTELLIER V, AUBRY L, GATELLIER P. Effect of oxidation on in vitro digestibility of skeletal muscle myofibrillar proteins[J]. J Agric Food Chem, 2007, 55(13): 5343-5348.
    [28]
    HILLER B, LORENZEN P C. Functional properties of milk proteins as affected by Maillard reaction induced oligomerisation[J]. Food Res Int, 2010, 43(4): 1155-1166.
    [29]
    马璐凯, 张宾, 王强, 等. 海藻糖、海藻胶及寡糖对南美白对虾蛋白质冷冻变性的抑制作用[J]. 现代食品科技, 2014, 30(6): 140-145.
    [30]
    肖连冬, 程爽, 李杰. 大豆分离蛋白起泡性和乳化性影响因素的研究[J]. 中国酿造, 2014, 33(4): 83-86.
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