Effect of small peptide supplementation at different protein levels on growth performance, digestive enzymes activities, serum biochemical indices and antioxidant abilities of grouper (<i>Epinephelus akaara</i>)

ZHAO Shuyan; LIN Heizhao; HUANG Zhong; ZHOU Chuanpeng; WANG Jun; WANG Yun; QI Changle; YANG Xiaoli; LIAO Jingqiu

doi:10.3969/j.issn.2095-0780.2016.03.003

Volume 12 Issue 3

Jun. 2016

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South China Fisheries Science > 2016 > 12(3): 15-23. > DOI: 10.3969/j.issn.2095-0780.2016.03.003

ZHAO Shuyan, LIN Heizhao, HUANG Zhong, ZHOU Chuanpeng, WANG Jun, WANG Yun, QI Changle, YANG Xiaoli, LIAO Jingqiu. Effect of small peptide supplementation at different protein levels on growth performance, digestive enzymes activities, serum biochemical indices and antioxidant abilities of grouper (Epinephelus akaara)[J]. South China Fisheries Science, 2016, 12(3): 15-23. DOI: 10.3969/j.issn.2095-0780.2016.03.003

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Effect of small peptide supplementation at different protein levels on growth performance, digestive enzymes activities, serum biochemical indices and antioxidant abilities of grouper (Epinephelus akaara)

1.
Guangdong Provincial Key Lab.of Fishery Ecology and Environment; Key Lab.of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
2.
School of Life Science and Technology, Shanghai Ocean University, Shanghai 201306, China
3.
Key Lab.of Aquatic Animal Breeding and Culturing for South China Sea, Ministry of Agriculture, Zhanjiang 52400, China

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Received Date: August 18, 2015
Revised Date: October 10, 2015

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Abstract

Abstract

Six diet groups containing protein (44%, 48%, 52%) and small peptide (0.05% and 0.1%) were fed to groupers (Epinephelus akaara) to assess the effect on growth performance, digestive enzymes activities, serum biochemical and antioxidant abilities. The diets were named as D1 (44%, 0.05%), D2 (44%, 0.1%), D3 (48%, 0.05%), D4 (48%, 0.1%), D5 (52%, 0.05%) and D6 (52%, 0.1%). Each diet was fed to triplicate groups of fish with 15 fish [initial weight (42.16±0.23) g] which were randomly sorted into each rearing cubic fiberglass tanks and the experimental last for eight weeks. The results show that there was no effect on weight gain (WG), specific growth rate (SGR), muscle moisture, crude protein and ash contents. As protein level and small peptide concentration increased, the crude fats of grouper muscle, triglyceride (TG) and cholesterol (CHO) decreased (P < 0.05). The activity of pepsin was the highest in Group D2, and the activities of amylase, trypsin and lipase were the highest in Group D1.There were no significant difference among different groups (P > 0.05). The activities of malondialdehyde (MDA) and peroxidase (POD) were not significantly different among different groups (P > 0.05). The result shows that supplementation of small peptide at different protein levels can reduce protein requirement, promote fat metabolism and increase digestive enzymes activities.
- Epinephelus akaara,
- small peptide,
- dietary protein,
- growth,
- serum biochemical indices,
- antioxidant abilities

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Effect of small peptide supplementation at different protein levels on growth performance, digestive enzymes activities, serum biochemical indices and antioxidant abilities of grouper (Epinephelus akaara)

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