Volume 8 Issue 6
Dec.  2012
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WEI Ya, ZHAO Yongqiang, HAO Shuxian, CEN Jianwei, HUANG Hui, LI Laihao. Comparison of carbazole method and phloroglucinol method for chondroitin sulfate determination[J]. South China Fisheries Science, 2012, 8(6): 65-71. DOI: 10.3969/j.issn.2095-0780.2012.06.010
Citation: WEI Ya, ZHAO Yongqiang, HAO Shuxian, CEN Jianwei, HUANG Hui, LI Laihao. Comparison of carbazole method and phloroglucinol method for chondroitin sulfate determination[J]. South China Fisheries Science, 2012, 8(6): 65-71. DOI: 10.3969/j.issn.2095-0780.2012.06.010
shu

Comparison of carbazole method and phloroglucinol method for chondroitin sulfate determination

  • 1.

    Key Lab. of Aquatic Product Processing, Ministry of Agriculture; National R & D Center for Aquatic Product Processing; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

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  • Received Date: March 06, 2012
  • Revised Date: June 20, 2012
    Graphical Abstract
    • Abstract

  • We compared the determinations of the concentrations of hydrolyzed and colored chondroitin sulfate (CS) by carbazole method and phloroglucinol method. The results reveal that the CS concentration by carbazole method and phloroglucinol method showed good linear relationship with their absorbance values within the ranges of 0.01~0.05 mg·mL-1 and 0.1~1.0 mg·mL-1, respectively. After 6 parallel tests on the same sample, the relative standard deviations (RSD) by carbazole method and phloroglucinol method were 3.90% and 4.28%, respectively, both with high precision. The two methods were spiked with 3 levels; the recoveries of carbazole method and phloroglucinol method were 98.0%~102.5% and 83.2%~106.6%, respectively. Thus, both carbazole method and phloroglucinol method can determine CS concentration accurately.

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