Volume 8 Issue 6
Dec.  2012
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WEI Ya, ZHAO Yongqiang, HAO Shuxian, CEN Jianwei, HUANG Hui, LI Laihao. Comparison of carbazole method and phloroglucinol method for chondroitin sulfate determination[J]. South China Fisheries Science, 2012, 8(6): 65-71. DOI: 10.3969/j.issn.2095-0780.2012.06.010
Citation: WEI Ya, ZHAO Yongqiang, HAO Shuxian, CEN Jianwei, HUANG Hui, LI Laihao. Comparison of carbazole method and phloroglucinol method for chondroitin sulfate determination[J]. South China Fisheries Science, 2012, 8(6): 65-71. DOI: 10.3969/j.issn.2095-0780.2012.06.010
shu

Comparison of carbazole method and phloroglucinol method for chondroitin sulfate determination

  • 1.

    Key Lab. of Aquatic Product Processing, Ministry of Agriculture; National R & D Center for Aquatic Product Processing; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China

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  • Received Date: March 06, 2012
  • Revised Date: June 20, 2012
    Graphical Abstract
    • Abstract

  • We compared the determinations of the concentrations of hydrolyzed and colored chondroitin sulfate (CS) by carbazole method and phloroglucinol method. The results reveal that the CS concentration by carbazole method and phloroglucinol method showed good linear relationship with their absorbance values within the ranges of 0.01~0.05 mg·mL-1 and 0.1~1.0 mg·mL-1, respectively. After 6 parallel tests on the same sample, the relative standard deviations (RSD) by carbazole method and phloroglucinol method were 3.90% and 4.28%, respectively, both with high precision. The two methods were spiked with 3 levels; the recoveries of carbazole method and phloroglucinol method were 98.0%~102.5% and 83.2%~106.6%, respectively. Thus, both carbazole method and phloroglucinol method can determine CS concentration accurately.

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    • References (21)
  • [1]
    李瑞国. 硫酸软骨素不同生产工艺的考察[J]. 中国医药工业杂志, 2003, 34(5): 221-222. https://www.doc88.com/p-4147988728578.html

    LI Ruiguo. Exploration of different process for production of chondroitin sulfate[J]. Chin J Pharm, 2003, 34(5): 221-222. (in Chinese) https://www.doc88.com/p-4147988728578.html
    [2]
    齐敬总, 闫晓玲, 王凤山. 硫酸软骨素含量测定方法介绍[J]. 中国生化药物杂志, 2002, 23(3): 162-164. doi: 10.3969/j.issn.1005-1678.2002.03.025

    QI Jingzong, YAN Xiaoling, WANG Fengshan. Introduction of the determination methods of chondroitin sulfate[J]. Chin J Biochem Pharm, 2002, 23(3): 162-164. (in Chinese) doi: 10.3969/j.issn.1005-1678.2002.03.025
    [3]
    NISHIDA K. Tissue engineering of the cornea[J]. Cornea, 2003, 22(7): 28-34. doi: 10.1097/00003226-200310001-00005
    [4]
    余洪华, 邓金印. 干眼诊治的临床研究[J]. 国际眼科杂志, 2006, 6(5): 1179-1180.

    YU Honghua, DENG Jinyin. The clinical research on diagnosis and treatment of dry eye[J]. Int J Ophthalmol, 2006, 6(5): 1179-1180. (in Chinese)
    [5]
    CLEGG D O, REDA D J, HARRIS C L, et al. Glucosamine, chondroitin sulfate, and the two in combination for painful knee osteoarthritis[J]. New Engl J Med, 2006, 354(8): 795-808. doi: 10.1056/NEJMoa052771
    [6]
    CAMEJO G, HURT E, WIKLUND O, et al. Modifications of low-density lipoprotein induced by arterial proteoglycans and chondroitin-6-sulfate[J]. Biochimicaet Biophysica Acta, 1991, 1069(3): 253-261. doi: 10.1016/0925-4439(91)90013-y
    [7]
    李南. 硫酸软骨素的开发及生产工艺控制[J]. 上海水产大学学报, 1998, 7(1): 38-43. https://qikan.cqvip.com/Qikan/Article/Detail?id=3139813

    LI Nan. Development and processing control of chondroitin sulfate[J]. J Shanghai Fish Univ, 1998, 7(1): 38-43. (in Chinese) https://qikan.cqvip.com/Qikan/Article/Detail?id=3139813
    [8]
    TÍMÁR J, DICZHÁZI C, BARTHA I, et al. Modulation of heparan-sulphate/chondroitin-sulphate ratio by glycosaminoglycan biosynthesis inhibitors affects liver metastatic potential of tumor cells[J]. Int J Cancer, 1995, 62(6): 755-761. doi: 10.1002/ijc.2910620618
    [9]
    CURATELLA B, BARTOLINI B, DI CARO A, et al. Sepharose-bound, highly sulfated glycosaminoglycans can capture HIV-1 from culture medium[J]. Carbohydr Res, 2005, 340(4): 759-764. doi: 10.1016/j.carres.2004.12.017
    [10]
    俆庆阳, 黎兴荣, 石墨, 等, 硫酸软骨素研究现状[J]. 生物技术通讯, 2006, 15(6): 633-635. https://qikan.cqvip.com/Qikan/Article/Detail?id=11738133&from=Qikan_Search_Index

    XU Qingyang, LI Xingrong, SHI Mo, et al. Chondroitin sulfate research[J]. Biotechnology, 2006, 15(6): 633-635. (in Chinese) https://qikan.cqvip.com/Qikan/Article/Detail?id=11738133&from=Qikan_Search_Index
    [11]
    牛增元, 张小吐, 刘钢, 等. 反相离子对高效液相色谱法测定硫酸软骨素[J]. 化学分析计量, 2002, 11(4): 7-9. https://qikan.cqvip.com/Qikan/Article/Detail?id=6544549&from=Qikan_Search_Index

    NIU Zengyuan, ZHANG Xiaotu, LIU Gang, et al. Reversed-phase ion-pair HPLC determination of chondroitin sulfate[J]. Chem Anal, 2002, 11(4): 7-9. (in Chinese) https://qikan.cqvip.com/Qikan/Article/Detail?id=6544549&from=Qikan_Search_Index
    [12]
    夏传俊, 孟菁, 杨耿. 反相高效液相色谱法测定硫酸软骨素的含量[J]. 安徽师范大学学报: 自然科学版, 2000, 23(4): 354-355. https://qikan.cqvip.com/Qikan/Article/Detail?id=4523987&from=Qikan_Search_Index

    XIA Chuanjun, MENG Jing, YANG Geng. RP-HPLC method for the determination of chondroitin sulfate[J]. Anhui Normal Univ: Natural Science, 2000, 23(4): 354-355. (in Chinese) https://qikan.cqvip.com/Qikan/Article/Detail?id=4523987&from=Qikan_Search_Index
    [13]
    茅力. 离子色谱法测定硫酸软骨素的含量[J]. 中国药科大学学报, 1998, 29(5): 358-361. https://qikan.cqvip.com/Qikan/Article/Detail?id=3305740&from=Qikan_Search_Index

    MAO Li. Ion chromatographic determination of the content of chondroitin sulfate[J]. China Pharm Univ, 1998, 29(5): 358-361. (in Chinese) https://qikan.cqvip.com/Qikan/Article/Detail?id=3305740&from=Qikan_Search_Index
    [14]
    茅力, 吕志宏, 陈景衡. 间接原子吸收法测定鲨鱼软骨粉中硫酸软骨素[J]. 中华预防医学杂志, 1998, 32(3): 183-184. https://qikan.cqvip.com/Qikan/Article/Detail?id=2991969&from=Qikan_Search_Index

    MAO Li, LV Zhihong, CHEN Jingheng. Indirect atomic absorption method for the determination of chondroitin sulfate, shark cartilage powder[J]. J Preventive Med, 1998, 32(3): 183-184. (in Chinese) https://qikan.cqvip.com/Qikan/Article/Detail?id=2991969&from=Qikan_Search_Index
    [15]
    施文健, 祝信贤. 碱性艳蓝BO分光光度法测定硫酸软骨素[J]. 中国生化药物杂志, 2004, 25(1): 28-30. https://qikan.cqvip.com/Qikan/Article/Detail?id=9190458&from=Qikan_Search_Index

    SHI Wenjian, ZHU Xinxian. Pure blue BO spectrophotometric determination of chondroitin sulfate[J]. Chin J Biochem Pharm, 2004, 25(1): 28-30. (in Chinese) https://qikan.cqvip.com/Qikan/Article/Detail?id=9190458&from=Qikan_Search_Index
    [16]
    丁雅勤, 孙伟, 高瑞芳, 等. 吖啶橙分光光度法测定硫酸软骨素含量[J]. 中国生化药物杂志, 2006, 27(2): 68-70. https://qikan.cqvip.com/Qikan/Article/Detail?id=21657970&from=Qikan_Search_Index

    DING Yaqin, SUN Wei, GAO Ruifang, et al. Acridine orange spectrophotometric determination of chondroitin sulfate[J]. Chin J Biochem Pharm, 2006, 27(2): 68-70. (in Chinese) https://qikan.cqvip.com/Qikan/Article/Detail?id=21657970&from=Qikan_Search_Index
    [17]
    陈媛媛, 蒋治良, 李振中, 等. 维多利亚蓝B分光光度法测定硫酸软骨素[J]. 光谱学与光谱分析, 2006, 26(6): 1148-1150. https://qikan.cqvip.com/Qikan/Article/Detail?id=22169541&from=Qikan_Search_Index

    CHEN Yuanyuan, JIANG Zhiliang, LI Zhenzhong, et al. Victoria blue B spectrophotometric determination of chondroitin sulfate[J]. Spectros Spect Anal, 2006, 26(6): 1148-1150. (in Chinese) https://qikan.cqvip.com/Qikan/Article/Detail?id=22169541&from=Qikan_Search_Index
    [18]
    高照明, 张玉冰, 于永良. 核磁共振内标法定量分析肝素钠中多硫酸软骨素[J]. 分析化学, 2011, 39(4): 601-602. https://qikan.cqvip.com/Qikan/Article/Detail?id=37332036&from=Qikan_Search_Index

    GAO Zhaoming, ZHANG Yubing, YU Yongliang. Analysis of oversulfated chondroitin sulfate in contaminated heparin by 1H-NMR internal standard method[J]. Chin J Anal Chem, 2011, 39(4): 601-602. (in Chinese) https://qikan.cqvip.com/Qikan/Article/Detail?id=37332036&from=Qikan_Search_Index
    [19]
    HOMERK A, DENBOW L, BEIGHTON D. Spectrophotometric method for assay of glycosam in-oglycans and glycosam inpglycan depolymerzin enzymes[J]. Anal Biochem, 1993, 214: 435-441.
    [20]
    杜美菊, 凌翠霞, 丁秀云. 中性红光法度测定硫酸软骨素含量[J]. 理化检验-化学分册, 2006, 42(8): 662-663. https://qikan.cqvip.com/Qikan/Article/Detail?id=22588262&from=Qikan_Search_Index

    DU Meiju, LING Cuixia, DING Xiuyun. Neutral red testimonies determination of chondroitin sulfate[J]. TESTING: Chem Anal, 2006, 42(8): 662-663. (in Chinese) https://qikan.cqvip.com/Qikan/Article/Detail?id=22588262&from=Qikan_Search_Index
    [21]
    LAUDER R, HUCKERBY T, NIEDUSZYNSK I J. A fingerprinting method for chondroit in dematan sulphate determination of hyaluronan and oligosaecharides[J]. Glyrobiology, 2000, 10(4): 393-401.
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