Citation: | LI Hongting, ZHANG Shuai, ZOU Keshu, CHEN Zuozhi, CHEN Xiaolei, JIANG Peiwen, CAO Yiting, LI Min. Establishment and optimization of environmental DNA extraction method from water of Pearl River Estuary[J]. South China Fisheries Science, 2022, 18(3): 30-37. DOI: 10.12131/20210304 |
[1] |
沈梅, 肖能文, 卢林, 等. 环境DNA检测鱼类的方法及应用 [J/OL]. 水生态学杂志, 2022. DOI: 10.15928/j.1674-3075.202103040058.
|
[2] |
单秀娟, 李苗, 王伟继. 环境DNA (eDNA) 技术在水生生态系统中的应用研究进展[J]. 渔业科学进展, 2018, 39(3): 23-29.
|
[3] |
BAKER C S, STEEL D, NIEUKIRK S, et al. Environmental DNA (eDNA) from the wake of the whales: droplet digital PCR for detection and species identification[J]. Front Mar Sci, 2018, 5: 133. doi: 10.3389/fmars.2018.00133
|
[4] |
MATEJUSOVA I, GRAHAM J, BLAND F, et al. Environmental DNA based surveillance for the highly invasive carpet sea squirt didemnum vexillum: a targeted single-species approach[J]. Front Mar Sci, 2021, 8: 1158.
|
[5] |
BLATTNER L, EBNER J N, ZOPFI J, et al. Targeted non-invasive bioindicator species detection in eDNA water samples to assess and monitor the integrity of vulnerable alpine freshwater environments[J]. Ecol Indic, 2021, 129: 107916. doi: 10.1016/j.ecolind.2021.107916
|
[6] |
LI M, SHAN X J, WANG W J, et al. Qualitative and quantitative detection using eDNA technology: a case study of Fenneropenaeus chinensis in the Bohai Sea[J]. Aquac Fish, 2020, 5(3): 148-155.
|
[7] |
李苗, 单秀娟, 王伟继, 等. 环境 DNA 在水体中存留时间的检测研究−以中国对虾为例[J]. 渔业科学进展, 2020, 41(1): 51-57.
|
[8] |
孙晶莹, 杨江华, 张效伟. 环境DNA (eDNA)宏条形码技术对枝角类浮游动物物种鉴定及其生物量监测研究[J]. 生态毒理学报, 2018, 13(5): 76-86. doi: 10.7524/AJE.1673-5897.20180108001
|
[9] |
周天成, 胡思敏, 林先智, 等. 基于 18S rDNA 条形码技术的珊瑚礁区塔形马蹄螺(Tectus pyramis)食性分析[J]. 海洋科学, 2020, 44(2): 99-107. doi: 10.11759/hykx20190117002
|
[10] |
ZHANG H, XU Q, ZHAO Y, et al. Sea cucumber (Apostichopus japonicus) eukaryotic food source composition determined by 18S rDNA barcoding[J]. Mar Biol, 2016, 163(7): 1-11.
|
[11] |
NORGAARD L, OLESEN C R, TROJELSGAARD K, et al. eDNA metabarcoding for biodiversity assessment, generalist predators as sampling assistants[J]. Sci Rep, 2021, 11(1): 6820. doi: 10.1038/s41598-021-85488-9
|
[12] |
王晨, 陶孟, 李爱民, 等. 基于环境DNA宏条形码技术的秦淮河生物多样性探究[J]. 生态学报, 2022, 42(2): 611-624.
|
[13] |
TABERLET P, COISSAC E, HAJIBABAEI M, et al. Environmental DNA[J]. Mol Ecol, 2012, 21(8): 1789-1793. doi: 10.1111/j.1365-294X.2012.05542.x
|
[14] |
FICETOLA G F, PANSU J, BONIN A, et al. Replication levels, false presences and the estimation of the presence/absence from eDNA metabarcoding data[J]. Mol Ecol Resour, 2015, 15: 543-556. doi: 10.1111/1755-0998.12338
|
[15] |
PIGGOTT M P. Evaluating the effects of laboratory protocols on eDNA detection probability for an endangered freshwater fish[J]. Ecol Evol, 2016, 6(9): 2739-2750. doi: 10.1002/ece3.2083
|
[16] |
EICHMILLER J J, MILLER L M, SORENSEN P W. Optimizing techniques to capture and extract environmental DNA for detection and quantification of fish[J]. Mol Ecol Res, 2016, 16(1): 56-68. doi: 10.1111/1755-0998.12421
|
[17] |
陈治, 宋娜, 源利文, 等. 舟山近海水样环境DNA获取方法的建立[J]. 水生生物学报, 2020, 44(1): 50-58. doi: 10.7541/2020.007
|
[18] |
黎慧, 阚霞, 魏宁, 等. 一种水环境eDNA提取方法的建立[J]. 安徽农业科学, 2019, 47(9): 108-110,115. doi: 10.3969/j.issn.0517-6611.2019.09.032
|
[19] |
DEINER K, WALSER J C, MӒCHLER E, et al. Choice of capture and extraction methods affects detection of freshwater biodiversity from environmental DNA[J]. Biol Conserv, 2015, 183: 53-63. doi: 10.1016/j.biocon.2014.11.018
|
[20] |
李苗, 单秀娟, 王伟继, 等. 中国对虾生物量评估的环境DNA检测技术的建立及优化[J]. 渔业科学进展, 2019, 40(1): 12-19.
|
[21] |
JEFFERSON T A, MOORE J E. Abundance and trends of Indo-Pacific finless porpoises (Neophocaena phocaenoides) in Hong Kong waters[J]. Front Mar Sci, 2020, 7: 1141.
|
[22] |
CHEN T, HUNG S K, QIU Y S, et al. Distribution, abundance, and individual movements of Indo-Pacific humpback dolphins (Sousa chinensis) in the Pearl River Estuary, China[J]. Mammalia, 2010, 74(2): 117-125.
|
[23] |
曾丹娜, 牛丽霞, 陶伟, 等. 夏季珠江口水域营养盐分布特征及其富营养化评价[J]. 广东海洋大学学报, 2020, 40(3): 73-82. doi: 10.3969/j.issn.1673-9159.2020.03.010
|
[24] |
WILSON I G. Inhibition and facilitation of nucleic acid amplification[J]. Appl Environ Microb, 1997, 63: 3741-3751. doi: 10.1128/aem.63.10.3741-3751.1997
|
[25] |
SPENS J, EVANS A R, HALFMAERTEN D, et al. Comparison of capture and storage methods for aqueous macrobial eDNA using an optimized extraction protocol: advantage of enclosed filter[J]. Methods Ecol Evol, 2016, 8(5): 635-645.
|
[26] |
GOLDBERG C S, TURNER C R, DEINER K, et al. Critical considerations for the application of environmental DNA methods to detect aquatic species[J]. Methods Ecol Evol, 2016, 7(11): 1299-1307. doi: 10.1111/2041-210X.12595
|
[27] |
LIANG Z, KEELEY A. Filtration recovery of extracellular DNA from environmental water samples[J]. Environ Sci Technol, 2013, 47(16): 9324-9331. doi: 10.1021/es401342b
|
[28] |
吴昀晟, 唐永凯, 李建林, 等. 环境DNA在长江江豚监测中的应用[J]. 中国水产科学, 2019, 26(1): 124-132.
|
[29] |
STRICKLER K M, FREMIER A K, GOLDBERG C S. Quantifying effects of UV-B, temperature, and pH on eDNA degradation in aquatic microcosms[J]. Biol Conserv, 2015, 183: 85-92. doi: 10.1016/j.biocon.2014.11.038
|
[30] |
MAJANEVA M, DISERUD O H, EAGLE S H C, et al. Environmental DNA filtration techniques affect recovered biodiversity[J]. Sci Rep, 2018, 8: 4682. doi: 10.1038/s41598-018-23052-8
|
[31] |
GOLDBERG C S, PILLIOD D S, ARKLE R S, et al. Molecular detection of vertebrates in stream water: a demonstration using Rocky Mountain tailed frogs and Idaho giant salamanders[J]. PLOS ONE, 2011, 6(7): e22746. doi: 10.1371/journal.pone.0022746
|
[32] |
PILLIOD D S, GOLDBERG C S, ARKLE R S. et al. Estimating occupancy and abundance of stream amphibians using environmental DNA from filtered water samples[J]. Can J Fish Aquat Sci, 2013, 70(8): 1123-1130. doi: 10.1139/cjfas-2013-0047
|
[33] |
STEWART K, MA H, ZHENG J, et al. Using environmental DNA to assess population-wide spatiotemporal reserve use[J]. Conserv Biol, 2017, 31(5): 1173-1182. doi: 10.1111/cobi.12910
|
[34] |
RENSHAW M A, OLDS B P, JERDE C L, et al. The room temperature preservation of filtered environmental DNA samples and assimilation into a phenol-chloroform-isoamyl alcohol DNA extraction[J]. Mol Ecol Res, 2015, 15(1): 168-176. doi: 10.1111/1755-0998.12281
|
[35] |
MINAMOTO T, NAKA T, MOJI K, et al. Techniques for the practical collection of environmental DNA: filter selection, preservation, and extraction[J]. Limnology, 2015, 17(1): 23-32.
|
[36] |
陈治, 陈建威, 王晓艳, 等. 舟山近海环境DNA保存方法的建立及优化[J]. 海洋与湖沼, 2019, 50(5): 1098-1107. doi: 10.11693/hyhz20190200034
|
[37] |
THOMSEN P F, KIELGAST J, IVERSEN L L, et al. Detection of a diverse marine fish fauna using environmental DNA from seawater samples[J]. PLOS One, 2012, 7(8): e41732. doi: 10.1371/journal.pone.0041732
|
[38] |
YAMANAKA, H, MOTOZAWA H, TSUJI S, et al. On-site filtration of water samples for environmental DNA analysis to avoid DNA degradation during transportation[J]. Ecol Res, 2016, 31(6): 963-967. doi: 10.1007/s11284-016-1400-9
|
[39] |
TAKAHARA T, MINAMOTO T, DOI H. Effects of sample processing on the detection rate of environmental DNA from the common carp (Cyprinus carpio)[J]. Biol Conserv, 2015, 183: 64-69. doi: 10.1016/j.biocon.2014.11.014
|
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![]() | |
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![]() | |
30. |
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![]() | |
31. |
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![]() | |
32. |
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![]() | |
33. |
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![]() | |
34. |
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![]() |