Citation: | WANG Chenglong, GUAN Wenzhi, LI Yongqiang, LIU Feng. Study on 17β-estradiol induced feminization of Pelteobagrus fulvidraco[J]. South China Fisheries Science, 2020, 16(3): 25-30. DOI: 10.12131/20200001 |
In this study, we compared the feeding method and feeding time of 17β-estradiol to explore the feminization method of XY Pelteobagrus fulvidraco, and then established three families (XY♀×XY♂) to compare the survival rate and sex reversal rate with sex molecular. The results show that the females in Artemia sp. group could not be induced to develop well from the 5th day of opening, but the induced reversal rate was 75.20% on the 1st day of opening, the survival rate induced from the 1st day of opening was not significantly different from that of Artemia sp. group, while the sex reversal rate was significantly higher than that of Artemia group; the combination of molecular markers Y1 and Y2 could identify the genetic sex of P. fulvidraco accurately; there was no significant difference in the survival rate and sex ratio of each genotype among the three families in the control group, and the survival rate was 51.00%−56.67%. The proportion of XX female and XY male individuals were 90.91%−97.44% and 94.56%−95.96%, respectively. There was no significant difference in the survival rate and sex reversal rate among the three families in the induction group. The result shows that using 50 mg·kg−1 E2 mixed with micro pellet feed to induce 30 d from the 1st day of opening can screen out well-developed female XY/YY P. fulvidraco effectively by combination of genetic and phenotypic sex identification.
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