Citation: | WANG Chenglong, GUAN Wenzhi, LI Yongqiang, LIU Feng. Study on 17β-estradiol induced feminization of Pelteobagrus fulvidraco[J]. South China Fisheries Science, 2020, 16(3): 25-30. DOI: 10.12131/20200001 |
In this study, we compared the feeding method and feeding time of 17β-estradiol to explore the feminization method of XY Pelteobagrus fulvidraco, and then established three families (XY♀×XY♂) to compare the survival rate and sex reversal rate with sex molecular. The results show that the females in Artemia sp. group could not be induced to develop well from the 5th day of opening, but the induced reversal rate was 75.20% on the 1st day of opening, the survival rate induced from the 1st day of opening was not significantly different from that of Artemia sp. group, while the sex reversal rate was significantly higher than that of Artemia group; the combination of molecular markers Y1 and Y2 could identify the genetic sex of P. fulvidraco accurately; there was no significant difference in the survival rate and sex ratio of each genotype among the three families in the control group, and the survival rate was 51.00%−56.67%. The proportion of XX female and XY male individuals were 90.91%−97.44% and 94.56%−95.96%, respectively. There was no significant difference in the survival rate and sex reversal rate among the three families in the induction group. The result shows that using 50 mg·kg−1 E2 mixed with micro pellet feed to induce 30 d from the 1st day of opening can screen out well-developed female XY/YY P. fulvidraco effectively by combination of genetic and phenotypic sex identification.
[1] |
代云云, 袁永明, 袁媛, 等. 我国黄颡鱼产业生产现状与发展趋势分析[J]. 现代商贸工业, 2019, 23(9): 22-24.
|
[2] |
刘汉勤, 崔书勤, 侯昌春, 等. 从XY雌鱼雌核发育产生YY超雄黄颡鱼[J]. 水生生物学报, 2007, 31(5): 718-725. doi: 10.3321/j.issn:1000-3207.2007.05.018
|
[3] |
杨天毅, 熊阳, 丹成, 等. 利用鱼类性逆转技术创制黄颡鱼XX雄鱼的方法[J]. 水生生物学报, 2018, 42(5): 871-878. doi: 10.7541/2018.107
|
[4] |
WANG D, MAO H L, CHEN H X, et al. Isolation of Y- and X-linked SCAR markers in yellow catfish and application in the production of all-male populations[J]. Anim Genet, 2009, 40(6): 978-981. doi: 10.1111/j.1365-2052.2009.01941.x
|
[5] |
DAN C, MEI J, WANG D, et al. Genetic differentiation and efficient sex-specific marker development of a pair of Y and X-linked markers in yellow catfish[J]. Int J Biol Sci, 2013, 9(10): 1043-1049. doi: 10.7150/ijbs.7203
|
[6] |
孙源. 黄颡鱼 (Pelteobagrus fulvidraco) 群体遗传结构分析及性别特异性分子标记的筛选[D]. 哈尔滨: 东北林业大学, 2011: 38-40.
|
[7] |
NAKADA N, NYUNOYA H, NAKAMURA M, et al. Identification of estrogenic compounds in wastewater effluent[J]. Environ Toxicol Chem, 2004, 23(12): 2807-2815. doi: 10.1897/03-699.1
|
[8] |
姚道霞. 黄颡鱼性分化及激素诱导性转化研究[D]. 哈尔滨: 东北林业大学, 2007: 34-35.
|
[9] |
HENDRY C I, MARTIN-ROBICHAUD D J, BENFEY T J. Hormonal sex reversal of Atlantic halibut (Hippoglossus hippoglossus L.)[J]. Aquaculture, 2003, 219(1/2/3/4): 769-781.
|
[10] |
PARK I S, KIM J H, CHO S H, et al. Sex differentiation and hormonal sex reversal in the bagrid catfish Pseudobagrus fulvidraco (Richardson)[J]. Aquaculture, 2004, 232(1/2/3/4): 183-193.
|
[11] |
刘少军, 刘筠. 雌二醇诱导革胡子鲇性转化及性腺观察[J]. 湖南师范大学自然科学学报, 1994, 17(1): 75-79.
|
[12] |
游鑫, 邓闵, 刘全圣, 等. 黄颡鱼性腺分化及温度对性别分化的影响[J]. 华中农业大学学报, 2016, 35(1): 106-113.
|
[13] |
FUZZEN M L M, BERNIER N J, van der KRAAK G. Differential effects of 17β-estradiol and 11-ketotestosterone on the endocrine stress response in zebrafish (Danio rerio)[J]. Gen Comp Endocrinol, 2001, 170(2): 365-373.
|
[14] |
JIN Y X, SHU L J, HUANG F Y. et al. Environmental cues influence EDC-mediated endocrine disruption effects in different developmental stages of Japanese medaka (Oryzias latipes)[J]. Aquat Toxicol, 2011, 101(1): 254-260. doi: 10.1016/j.aquatox.2010.10.005
|
[15] |
李国超. 17β-雌二醇对斑马鱼的毒性研究[D]. 北京: 中国农业科学院, 2015: 6-13
|
[16] |
周锋祺, 谢庆平, 楼宝, 等. 17β-雌二醇浸浴对小黄鱼早期生长及性腺发育的影响[J]. 浙江海洋大学学报, 2018, 37(4): 302-307.
|
[17] |
CARVALHO C V A, PASSINI G, COSTA W M, et al. Effect of estradiol-17β on the sex ratio, growth and survival of juvenile common snook (Centropomus undecimalis)[J]. Acta Sci Anim Sci, 2014, 36(3): 239-245. doi: 10.4025/actascianimsci.v36i3.22839
|
[18] |
BLAZQUEZ M, ZANUY S, CARRILLO M, et al. Structural and functional effects of early exposure to estradiol-17β and 17α-ethynylestradiol on the gonads of the gonochoristic teleost Dicentrarchus labrax[J]. Fish Physiol Biochem, 1998, 18(1): 37-47. doi: 10.1023/A:1007736110663
|
[19] |
MALISON J A, KAYES T B, WENTWORTH B C, et al. Growth and feeding responses of male versus female yellow perch (Perca flavescens) treated with estradiol-17β[J]. Can J Fish Aquat Sci, 1988, 45(11): 1942-1948. doi: 10.1139/f88-226
|
[20] |
张晓彦, 刘海金. 17β-雌二醇对半滑舌鳎性分化和生长的影响[J]. 东北农业大学学报, 2009, 40(6): 67-72. doi: 10.3969/j.issn.1005-9369.2009.06.014
|
[21] |
PIFERRER F, RIBAS L, DIAZ N. Genomic approaches to study genetic and environmental influences on fish sex determination and differentiation[J]. Mar Biotechnol (NY), 2012, 14: 591-604. doi: 10.1007/s10126-012-9445-4
|
[22] |
BROWN E E, BAUMANN H, CONOVER D O. Temperature and photoperiod effects on sex determination in a fish[J]. J Exp Mar Biol Ecol, 2014, 461: 39-43. doi: 10.1016/j.jembe.2014.07.009
|
[23] |
HAYASHI Y, KOBIRA H, YAMAGUCHI T, et al. High temperature causes ma sculinization of genetically female medaka by elevation of cortisol[J]. Mol Reprod Dev, 2010, 77(8): 679-686. doi: 10.1002/mrd.21203
|
[24] |
RUBIN D A. Effect of pH on sex ratio in cichlids and a poecillid[J]. Copeia, 1985(1): 233-235.
|
[25] |
FRANCIS R C, BARLOW G W. Social control of primary sex differentiation in the Midsa cichlid[J]. Proc Natl Acad Sci USA, 1993, 90(22): 10673-10675. doi: 10.1073/pnas.90.22.10673
|
[26] |
OSPINA-ALVAREZ N, PIFERRER F. Temperature-dependent sex determination in fish revisited: prevalence, a single sex ratio response pattern, and possible effects of climate change[J]. PLoS One, 2008, 3: e2837. doi: 10.1371/journal.pone.0002837
|
[27] |
PAVLIDIS M, KOUMOUNDOUROS G, STERIOTI A, et al. Evidence of temperature-dependent sex determination in the European sea bass (Dicentrarchus labrax L.)[J]. J Exp Zool, 2000, 287: 225-232. doi: 10.1002/1097-010X(20000801)287:3<225::AID-JEZ4>3.0.CO;2-D
|
[28] |
MATSUDA M, NAGAHAMA Y, SHINOMIYA A, et al. DMY is a Y-specific DM-domain gene required for male development in the medaka fish[J]. Nature, 2002, 417: 559-563. doi: 10.1038/nature751
|
[29] |
MASUYAMA H, YAMADA M, KAMEI Y, et al. Dmrt1 mutation causes a male-to-female sex reversal after the sex determination by Dmy in the medaka[J]. Chromosome Res, 2012, 20: 163-176. doi: 10.1007/s10577-011-9264-x
|
[30] |
TAKEHANA Y, MATSUDA M, MYOSHO T, et al. Co-option of Sox3 as the male-determining factor on the Y chromosome in the fish Oryzias dancena[J]. Nat Commun, 2014, 5: 1-10.
|
[31] |
谢彬月, 熊舒婷, 梅杰. 黄颡鱼性别决定和分化相关基因的鉴定及其在性腺中的表达分析[J]. 湖北农业科学, 2017, 56(12): 2362-2367.
|
[1] | YANG Bingzhong, YAN Lei, LI Jie, WANG Teng, ZHANG Peng. Size selectivity of codend-mesh size in single boat bottom otter trawls for Nemipterus virgatus in South China Sea[J]. South China Fisheries Science, 2024, 20(3): 1-7. DOI: 10.12131/20230227 |
[2] | WANG Yongjin, ZHANG Xun, ZHANG Yu, ZHOU Aizhong, LI Ziniu, WANG Shuaijie, LIU Longteng, WANG Lumin. Influence of main structural parameters on performance of bottom trawl with large-size mesh[J]. South China Fisheries Science, 2021, 17(4): 66-73. DOI: 10.12131/20210026 |
[3] | YANG Bingzhong, YANG Lin, TAN Yongguang, YAN Lei, ZHANG Peng, LI Jie. Size selectivity of combined square mesh and diamond mesh codends of shrimp beam trawl in South China Sea[J]. South China Fisheries Science, 2018, 14(1): 105-113. DOI: 10.3969/j.issn.2095-0780.2018.01.014 |
[4] | YAN Lei, LI Yanan, TAN Yongguang, YANG Lin, YANG Bingzhong, ZHANG Peng, CHEN Sen, LI Jie. Mesh size selectivity of Harpodon nehereus gillnet in northern South China Sea[J]. South China Fisheries Science, 2016, 12(2): 75-80. DOI: 10.3969/j.issn.2095-0780.2016.02.011 |
[5] | YANG Bingzhong, YANG Lin, TAN Yongguang, ZHANG Peng, YAN Lei, CHEN Sen. Preliminary analysis of relationship between mesh size of gillnet and body characteristics of target species in the South China Sea[J]. South China Fisheries Science, 2015, 11(6): 94-99. DOI: 10.3969/j.issn.2095-0780.2015.06.013 |
[6] | HUANG Xiaohua, GUO Genxi, TAO Qiyou, HU Yu. Numerical simulation of the forces and deformation of HDPE circular gravity cages[J]. South China Fisheries Science, 2013, 9(5): 126-131. DOI: 10.3969/j.issn.2095-0780.2013.05.019 |
[7] | YANG Bingzhong, YANG Lin, TAN Yongguang, ZHANG Peng, YAN Lei. Relationship between body characteristic of Scomberomorus and the mesh size[J]. South China Fisheries Science, 2013, 9(5): 120-125. DOI: 10.3969/j.issn.2095-0780.2013.05.018 |
[8] | ZHANG Xufeng, ZHANG Peng, YANG Lin, TAN Yonggunag. Comparison and analysis on catch selectivity of square mesh codends between 35.2 and 30.3 mm of trawl in northern South China Sea[J]. South China Fisheries Science, 2007, 3(2): 49-54. |
[9] | ZHANG Xu-feng, ZHANG Peng, TAN Yong-guang, YANG Lin. Analysis on catch selectivity of 30.3 mm square mesh codend oftrawl in Northern South China Sea[J]. South China Fisheries Science, 2006, 2(2): 51-55. |
[10] | GE Chang-zi. The review on the analysis of mesh size selectivity of trawl cod-end[J]. South China Fisheries Science, 2005, 1(4): 30-35. |