Volume 15 Issue 5
Oct.  2019
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TANG Yapeng, WANG Ruixuan, HUANG Jianhua, YANG Lishi, JIANG Shigui, LIN Heizhuo, WANG Guofu. Effects of two breeding methods on growth and bacterial structure of Nitzschia closterium f. minutissima[J]. South China Fisheries Science, 2019, 15(5): 55-62. DOI: 10.12131/20190011
Citation: TANG Yapeng, WANG Ruixuan, HUANG Jianhua, YANG Lishi, JIANG Shigui, LIN Heizhuo, WANG Guofu. Effects of two breeding methods on growth and bacterial structure of Nitzschia closterium f. minutissima[J]. South China Fisheries Science, 2019, 15(5): 55-62. DOI: 10.12131/20190011
shu

Effects of two breeding methods on growth and bacterial structure of Nitzschia closterium f. minutissima

  • 1.

    Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Fisheries, Tianjin Agricultural University, Tianjin 300384, China

  • 3.

    Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China

  • 4.

    Hainan Academy of Ocean and Fisheries Sciences, Haikou 571126, China

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  • Received Date: January 09, 2019
  • Revised Date: May 13, 2019
  • Accepted Date: June 23, 2019
  • Available Online: July 01, 2019
    Graphical Abstract
    • Abstract

  • We compared the density, pH, dissolved oxygen and colony structure of Nitzschia closterium f. minutissima bred in negative pressure photobioreactor and open bucket, respectively. It is shown that the algae grew fast in negative pressure photobioreactor with the highest density of 1.33×107 cells·mL–1, significantly higher than that in open bucket (8.36×106 cells·mL–1). The pH in algal solution increased with increase of algal density, and there was a significant positive correlation between them (P<0.01). The highest pH values in negative pressure photobioreactor and open bucket were 10.3 and 9.3, respectively. Oppositely, in negative pressure photobioreactor, the dissolve oxygen of algea decreased with increase of algal density, which finally stabilized at about 6.5 mg·L–1. The decline of dissolved oxygen might be related to the fact that Roseobacter had become the dominant bacteria. Results of 16S rDNA gene high-throughput sequencing show that the bacterial diversity decreased significantly (P<0.05). Proteobacteria and Bacteroidetes were dominant bacteria at early stage of breeding. However, the dominant bacteria were Cyanobacteria and Roseobacter at late stage in negative pressure photobioreactor, which was obviously different from that in open bucket.

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