Volume 15 Issue 1
Feb.  2019
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DENG Yiqin, CHEN Chang, SU Youlu, CEHNG Changhong, MA Hongling, GUO Zhixun, FENG Juan. Construction of knock-out mutant of sRNA srvg17985 in Vibrio alginolyticus ZJ-T and its preliminary function analysis[J]. South China Fisheries Science, 2019, 15(1): 42-53. DOI: 10.12131/20180127
Citation: DENG Yiqin, CHEN Chang, SU Youlu, CEHNG Changhong, MA Hongling, GUO Zhixun, FENG Juan. Construction of knock-out mutant of sRNA srvg17985 in Vibrio alginolyticus ZJ-T and its preliminary function analysis[J]. South China Fisheries Science, 2019, 15(1): 42-53. DOI: 10.12131/20180127
shu

Construction of knock-out mutant of sRNA srvg17985 in Vibrio alginolyticus ZJ-T and its preliminary function analysis

  • 1.

    Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Xisha and Nansha Ocean Observation and Research Station, South China Sea Institute of Oceanology, Chinese Academy of Sciences, Guangzhou 510301, China

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  • Received Date: June 11, 2018
  • Revised Date: August 01, 2018
  • Accepted Date: October 07, 2018
  • Available Online: December 04, 2018
    Graphical Abstract
    • Abstract

  • We constructed the sRNA srvg17985 knock-out mutant in Vibrio alginolyticus ZJ-T by using homologous recombination, so as to compare the biological characteristics of growth in LBS medium, motility, extracellular protease secretion, iron utilization, antibiotic resistance and metabolism between the wild type and the mutant strains. The results show that the absence of sRNA srvg17985 did not affect the growth in LBS medium, motility, extracellular protease secretion, iron utilization, antibiotic resistance and most of the tested carbon and nitrogen sources metabolism in V. alginolyticus. However, the mutant showed better utilization of laminarin, pectin and dihydroxyaceton, and gained the ability to utilize alanine-aspartic acid (Ala-Asp) as the sole nitrogen source.

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