Volume 13 Issue 3
May  2017
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CHEN Jinsong, JIANG Shigui, HUANG Jianhua, YANG Qibin, MA Zhenhua, ZHOU Falin. Molecular cloning and expression analysis of aspartate aminotransferase (AST) in Penaeus monodon under ambi-ent ammonia stress[J]. South China Fisheries Science, 2017, 13(3): 73-82. DOI: 10.3969/j.issn.2095-0780.2017.03.010
Citation: CHEN Jinsong, JIANG Shigui, HUANG Jianhua, YANG Qibin, MA Zhenhua, ZHOU Falin. Molecular cloning and expression analysis of aspartate aminotransferase (AST) in Penaeus monodon under ambi-ent ammonia stress[J]. South China Fisheries Science, 2017, 13(3): 73-82. DOI: 10.3969/j.issn.2095-0780.2017.03.010
shu

Molecular cloning and expression analysis of aspartate aminotransferase (AST) in Penaeus monodon under ambi-ent ammonia stress

  • 1.

    Key Lab.of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

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  • Received Date: March 06, 2016
  • Revised Date: May 26, 2016
  • Accepted Date: September 25, 2016
    Graphical Abstract
    • Abstract

  • To explore the function of aspartate aminotransferase gene in the process of the ammonia nitrogen metabolism of black tiger shrimps (Penaeus monodon), the full-length cDNA sequence of aspartate aminotransferase from P.monodon (PmAST) was obtained by high throughput transcriptome sequencing and rapid amplification of cDNA ends.On this basis, the expression of the PmAST in different tissues under different ambient ammonia stress were detected by fluorescence-quantitative real time PCR.The cDNA length of PmAST was 1 957 bp, including a 5′UTR of 131 bp and a 3′UTR of 584 bp.The length of the open reading frame (ORF) was 1 242 bp encoding a polypeptide of 413 amino acid.The molecular mass of the deduced amino acid (aa) sequence was 45.852 kD with an estimated pI of 8.85, and there was a tailing signal (poly A) with 30 bp length.Like other animals′ ASTs, the structure of PmAST protein included an AAT-I superfamily domain.There were 15 phosphorylation sites and 2 glycosylation sites in this protein.Analysis of the tissue expression pattern of the PmAST shows that PmAST mRNA was expressed in all tested tissues, including ovary, haemolymph, brain, lymph, stomach, muscle, thoracic ganglia, heart, hepatopancreas and gill.The PmAST expression reached the maximum value in hepatopancreas and was the lowest in brain.After ambient ammonia stress experiment, the expression of the PmAST in hepatopancreas and gill was significantly higher than that in the control (P < 0.05), and the expression profiles differed between hepatopancreas and gill.The result shows that the higher the ambient ammonia concentration is, the greater the increase of PmAST expression will be.Therefore, the PmAST takes part in acute ammonia stress.

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    • References (31)
  • [1]
    李少飞, 何玉英, 李吉涛, 等.中国明对虾天门冬氨酸转氨酶基因的克隆及氨氮胁迫对其时空表达的影响[J].中国水产科学, 2014, 21(6):1125-1133.
    [2]
    王镜岩, 朱胜庚, 徐长法.生物化学[M].3版.北京:高等教育出版社, 2002:306-308.
    [3]
    潘劲劲. 天冬氨酸转氨酶与血小板比值在转氨酶两倍以下慢性乙型肝炎病毒感染患者中的临床应用评价[D]. 安徽: 安徽医科大学, 2014: 8-9.
    [4]
    杜宗孝, 李富荣, 朴文花.血清天门冬氨酸氨基转移酶线粒体同工酶在酒精性肝病中的临床价值[J].检验医学, 2012, 27(9):732-735. http://med.wanfangdata.com.cn/Paper/Detail?id=PeriodicalPaper...
    [5]
    LIN L, ZENG X L, ZHANG J.Effect of profenofos poisoning on liver lipid peroxidation and liver function in rabbits[J].Chin J Clin Rehabilit, 2004, 8(21):4380-4381. https://www.researchgate.net/publication/286968630_Effect_of...
    [6]
    张彬彬.乙草胺对泥鳅肝脏谷丙转氨酶和谷草转氨酶的影响[J].江苏农业科学, 2009 (1):289-290.
    [7]
    李波, 樊启学, 杨凯, 等.慢性氨氮胁迫对黄颡鱼摄食, 生长及血液指标的影响[J].应用与环境生物学报, 2011, 17(6):824-828.
    [8]
    黄忠, 林黑着, 李卓佳, 等.复方中草药投喂策略对凡纳滨对虾生长, 消化及非特异性免疫功能的影响[J].南方水产科学, 2013, 9(5):37-43. http://www.wenkuxiazai.com/doc/4fb642ae7c1cfad6195fa7b2.html
    [9]
    MCKENNA M C, HOPKINS I B, LINDAUER S L, et al.Aspartate aminotransferase in synaptic and nonsynaptic mitochondria:differential effect of compounds that influence transient hetero-enzyme complex (metabolon) formation[J].Neurochem Int, 2006, 48(6):629-636. https://www.sciencedirect.com/science/article/pii/S0197018606000623
    [10]
    MCCUTCHEON J P, von DOHLEN C D.An interdependent metabolic patchwork in the nested symbiosis of mealybugs[J].Curr Biol, 2011, 21(16):1366-1372. doi: 10.1016/j.cub.2011.06.051
    [11]
    SCARAFFIA P Y, ISOE J, MURILLO A, et al.Ammonia metabolism in Aedes aegypti[J].Insect Biochem Molec, 2005, 35(5):491-503. doi: 10.1016/j.ibmb.2005.01.012
    [12]
    CHEN J, ZHOU F, HUANG J, et al.Ammonia and salinity tolerance of Penaeus monodon across eight breeding families[J].SpringerPlus, 2016, 5(1):1. doi: 10.1186/s40064-015-1659-2
    [13]
    韩春艳, 郑清梅, 陈桂丹, 等.氨氮胁迫对奥尼罗非鱼非特异性免疫的影响[J].南方水产科学, 2014, 10(3):47-52.
    [14]
    李少飞. 中国对虾氨氮代谢酶基因的cDNA克隆及其在氨氮解毒代谢过程中的作用[D]. 大连: 大连海洋大学, 2014: 28-30.
    [15]
    刘胜男, 潘鲁青, 刘茂琪.氨氮胁迫对三疣梭子蟹解毒代谢关键基因表达的影响[J].海洋湖沼通报, 2015 (2):97-104. http://www.cqvip.com/QK/95761X/201502/666585651.html
    [16]
    岳峰. 三疣梭子蟹在氨氮胁迫下免疫应答与解毒代谢机制的研究[D]. 青岛: 中国海洋大学, 2010: 11-12.
    [17]
    胡志国, 刘建勇, 袁瑞鹏, 等.凡纳滨对虾高氨氮和低溶氧抗逆性状的杂交配合力分析[J].南方水产科学, 2016, 12(1):43-49. http://www.cqvip.com/QK/60642B/201601
    [18]
    肖炜, 李大宇, 徐杨, 等.慢性氨氮胁迫对吉富罗非鱼幼鱼生长, 免疫及代谢的影响[J].南方水产科学, 2015, 11(4):81-87. http://www.cqvip.com/QK/60642B/201504/665703414.html
    [19]
    黄建华, 李永, 杨其彬, 等.斑节对虾家系氨氮耐受性的比较[J].南方水产科学, 2012, 8(6):37-43. http://www.oalib.com/paper/4365344
    [20]
    郑微云, 翁思琪.环境毒理学概论[M].厦门:厦门大学出版社, 1993:57-60.
    [21]
    ZANG W L, XU X C, DAI X L, et al.Toxic effects of Zn2+, Cu2+, Cd2+ and NH3 on Chinese prawn[J].Chin J Oceanol Limnol, 1993, 3:254-259.
    [22]
    荆晓丽. 青岛文昌鱼谷丙转氨酶基因克隆, 表达和功能研究[D]. 青岛: 中国海洋大学, 2010: 56-61.
    [23]
    MEHTA P K, HALE T I, CHRISTEN P.Aminotransferases:demonstration of homology and division into evolutionary subgroups[J].Eur J Biochem, 1993, 214(2):549-561. doi: 10.1111/ejb.1993.214.issue-2
    [24]
    陈玉春, 顾雪飞, 刘敏.5种中草药对鲤血清谷丙转氨酶, 谷草转氨酶及红细胞过氧化氢酶活性的影响[J].淡水渔业, 2007, 37(5):11-13. http://www.wenkuxiazai.com/doc/cd1e180f866fb84ae45c8d81.html
    [25]
    赵杰, 谷子林, 崔青曼, 等."克白克"对中国对虾体内琥珀酸脱氢酶, 磷酸甘油三酯和谷草转氨酶活性的影响[J].中国饲料, 2002(3):14. http://industry.wanfangdata.com.cn/dl/Magazine?magazineId=zgsl&...
    [26]
    REGNAULT M.Nitrogen excretion in marine and fresh-water crustacea[J].Biol Rev, 1987, 62(1):1-24. https://www.researchgate.net/publication/229992875_Nitrogen...
    [27]
    CHEN J C, LIN C Y.Responses of oxygen consumption, ammonia-N excretion and urea-N excretion of Penaeus chinensis exposed to ambient ammonia at different salinity and pH levels[J].Aquaculture, 1995, 136(3):243-255. https://es.scribd.com/document/153402238/2009-Rational-pdf
    [28]
    CHEN J M, CHEN J C.Study on the free amino acid levels in the hemolymph, gill, hepatopancreas and muscle of Penaeus monodon exposed to elevated ambient ammonia[J].Aquat Toxicol, 2000, 50(1):27-37.
    [29]
    WALTON D J.Stereochemistry of reduction of D-glyceraldehyde catalyzed by a nicotinamide adenine dinucleotide phosphate dependent dehydrogenase from skeletal muscle[J].Biochemistry, 1973, 12(18):3472-3478. doi: 10.1021/bi00742a018
    [30]
    李永. 斑节对虾人工选育及氨氮对其免疫, 解毒代谢影响[D]. 上海: 上海海洋大学, 2012: 9.
    [31]
    曾媛媛, 陈曦飞, 艾春香, 等. 氨氮胁迫对拟穴青蟹血淋巴生理生化指标及鳃和肝胰腺显微结构的影响[C]//中国甲壳动物学会第十一届年会暨学术研讨会论文摘要集. 厦门: 中国海洋湖沼学会, 2011: 107.
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