Tissue distribution and response to infection of Photobacterium damselae subsp. piscicida of Tf, TNFα and C-Lys in Trachinotus ovatus

SONG Shasha; MA Hongling; FENG Juan; SU Youlu; CHENG Changhong; GUO Zhixun

doi:10.3969/j.issn.2095-0780.2017.02.010

The study investigates the tissue expression of transferrin (Tf), tumor necrosis factor alpha (TNFα) and C-type lysozyme (C-Lys) in healthy Trachinotus ovatus after it was infected with Photobacterium damselae subsp. piscicida in kidney. The results show that after infection, the accumulative mortalities of T.ovatus reached the maximum in 48 h (76.4%); and the colony of P.damselae in T.ovatus′ kidney continued to decline in 3~96 h from 1.07×10⁶ CFU·g^-1to 1.29×10³ CFU·g^-1. The Tf and TNFα expressions were the highest in the liver, and the C-Lys expression was the highest in the head kidney in healthy T.ovatus. After TOS1 infected, the Tf expression in 3~12 h in test group was higher than that of control and was the highest in 6 h (17.99 times as high as that of control). The TNFα expression of the test group was higher than that of the control after infection of 3 h, 6 h and 24 h. The TNFα expression was the highest in 24 h (6.05 times higher than that of control). The C-Lys expression in infection group was lower than that in control in 3~96 h. The study indicates that the Tf and TNFα expressions can be promoted while C-Lys will be suppressed after TOS1 infection in T.ovatus′ kidney.

[1]	夏立群, 黄郁葱, 鲁义善.卵形鲳鲹主要病害及其研究进展[J].安徽农学通报:上半月刊, 2012, 18(23):140-143, 150. http://www.cqvip.com/QK/98504X/201223/44195030.html
[2]	苏友禄, 冯娟, 郭志勋, 等.美人鱼发光杆菌杀鱼亚种感染卵形鲳鲹的病理学观察[J].海洋科学, 2012, 36(2):75-81. http://d.wanfangdata.com.cn/periodical_hykx201202014.aspx
[3]	苏友禄, 冯娟, 郭志勋, 等.3种美人鱼发光杆菌疫苗对卵形鲳鲹的免疫效果[J].华南农业大学学报, 2011, 32(3):105-110. http://www.cqvip.com/QK/90891X/201103/38729166.html
[4]	蔡文超, 区又君, 李加儿, 等.卵形鲳鲹免疫器官的早期发育[J].南方水产科学, 2012, 8(5):39-45. http://www.schinafish.cn/CN/abstract/abstract8975.shtml
[5]	但学明, 李安兴, 林小涛, 等.卵形鲳鲹对刺激隐核虫的免疫应答和免疫保护研究[J].水生生物学报, 2008, 32(1):13-18. http://www.wenkuxiazai.com/doc/ba6443d5bb4cf7ec4afed06c.html
[6]	许海东, 区又君, 郭志勋, 等.神经坏死病毒对卵形鲳鲹的致病性及外壳蛋白基因序列分析[J].上海海洋大学学报, 2010, 19(4):482-488. http://www.cqvip.com/QK/90212A/201004/34631492.html
[7]	黄郁葱, 简纪常, 吴灶和, 等.卵形鲳鲹结节病病原的分离与鉴定[J].广东海洋大学学报, 2008, 28(4):49-53. doi: 10.3969/j.issn.1673-9159.2008.04.011
[8]	张飞, 苏永全, 王军, 等.大黄鱼(Pseudosciaena crocea)源美人鱼发光杆菌(Photobacterium damselae)的分离鉴定及致病性研究[J].海洋与湖沼, 2012, 43(6):1202-1208. doi: 10.11693/hyhz201206025025
[9]	张晓君, 秦国民, 陈翠珍, 等.龙胆石斑鱼源美人鱼发光杆菌的生物学特性与系统发育学分析[J].渔业科学进展, 2009, 30(3):38-43. http://www.wenkuxiazai.com/doc/6d2f32d280eb6294dd886c3b.html
[10]	任燕, 石存斌, 常藕琴, 等.五条鰤源美人鱼发光杆菌杀鱼亚种的分离鉴定及药物敏感试验[J].广东农业科学, 2012, 39(22):148-152. doi: 10.3969/j.issn.1004-874X.2012.22.049
[11]	WANG Y, HAN Y, LI Y, et al.Isolation of Photobacterium damselae subsp.piscicida from diseased tongue sole(Cynoglossus semilaevis Gunther) in China[J].微生物学报, 2007, 47(5):763-768.
[12]	LÓPEZ-DÓRIGA M V, BARNES A C, DOS SANTOS N M, et al.Invasion of fish epithelial cells by Photobacterium damselae subsp.piscicida: evidence for receptor specificity, and effect of capsule and serum[J].Microbiology, 2000, 146(Pt 1):21-30. http://downloads.hindawi.com/journals/jir/2014/273284.xml
[13]	龙华, 郑英.转铁蛋白生理功能及其动态模型[J].湖北农学院学报, 2004, 24(1):30-35. http://www.cnki.com.cn/Article/CJFDTOTAL-HBNX200401008.htm
[14]	高春萍. 黑鲷肿瘤坏死因子cDNA的克隆与体外表达的研究[D]. 青岛: 中国海洋大学, 2004: 2. http://cdmd.cnki.com.cn/Article/CDMD-10423-2004131525.htm
[15]	朱占英. 草鱼免疫细胞和免疫相关基因研究: 自然杀伤细胞杀伤活性和溶菌酶mRNA的表达[D]. 上海: 上海海洋大学, 2012: 5. http://cdmd.cnki.com.cn/Article/CDMD-10264-1013000340.htm
[16]	徐永平, 汪婷婷, 孙永欣, 等.水产动物溶菌酶研究的最新进展[J].水产科学, 2011, 30(5):307-310. http://industry.wanfangdata.com.cn/dl/Detail/Periodical?id=...
[17]	王瑞旋, 冯娟, 苏友禄, 等.卵形鲳鲹美人鱼发光杆菌杀鱼亚种的分离鉴定[J].中国水产科学, 2010, 17(5):1020-1027. http://www.cqvip.com/qk/98270X/201005/35237841.html
[18]	周小秋, 冯琳, 姜维丹, 等.营养与鱼类免疫研究进展[J].动物营养学报, 2014, 26(10):3175-3184. doi: 10.3969/j.issn.1006-267x.2014.10.032
[19]	王娜, 陈琼, 胡成钰.草鱼转铁蛋白基因的克隆及其组织表达[J].水生生物学报, 2010, 34(1):51-56. http://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper...
[20]	LUCK A N, MASON A B.Structure and dynamics of drug carriers and their interaction with cellular receptors: focus on serum transferrin[J].Adv Drug Deliv Rev, 2013, 65(8):1012-1019. doi: 10.1016/j.addr.2012.11.001
[21]	GAO J, DING S, HUANG X, et al.Cloning and expression characterization of the serum transferrin gene in the Chinese black sleeper (Bostrichthys sinensis)[J].Gene, 2013, 515(1):89-98. doi: 10.1016/j.gene.2012.11.022
[22]	OPPENHEIMER S J. Iron and its relation to immunity and infectious disease[J]. J Nutr, 2001, 131(2): 616S-635S.
[23]	高明英. 黄喉拟水龟转铁蛋白基因的克隆、表达及重组蛋白的抗菌性分析[D]. 上海: 上海海洋大学, 2011: 20-22. http://cdmd.cnki.com.cn/Article/CDMD-10264-1011305208.htm
[24]	李伟, 史燕, 赵建, 等.中华鳖转铁蛋白基因序列特征及表达研究[J].水生生物学报, 2014, 39(3):482-489. http://industry.wanfangdata.com.cn/yj/Detail/Periodical?id=...
[25]	张志伟, 李忠, 梁宏伟, 等.鲢转铁蛋白基因cDNA的克隆及其在胚胎期的表达分析[J].华中农业大学学报, 2011, 30(3):352-357. http://www.cqvip.com/QK/90088X/201103/38292877.html
[26]	RONZA P, LOSADA A P, VILLAMARÍN A, et al.Immunolocalization of tumor necrosis factor alpha in turbot (Scophthalmus maximus, L.) tissues[J].Fish Shellfish Immunol, 2015, 45(2):470-476. doi: 10.1016/j.fsi.2015.04.032
[27]	邱丽华, 宋林生, 蔡中华, 等.花鲈肿瘤坏死因子基因cDNA的克隆、分析与表达[J].中国水产科学, 2003, 10(5):370-375. http://www.cnki.com.cn/Article/CJFDTotal-ZSCK200305004.htm
[28]	KIM M S, HWANG Y J, YOON K J, et al.Molecular cloning of rock bream (Oplegnathus fasciatus) tumor necrosis factor-α and its effect on the respiratory burst activity of phagocytes[J].Fish Shellfish Immunol, 2009, 27(5):618-624. doi: 10.1016/j.fsi.2009.07.007
[29]	ZOU J, WANG T, HIRONO I, et al.Differential expression of two tumor necrosis factor genes in rainbow trout, Oncorhynchus mykiss[J].Dev Comp Immunol, 2002, 26(2):161-172. doi: 10.1016/S0145-305X(01)00058-1
[30]	KINOSHITA S, BISWAS G, KONO T, et al.Presence of two tumor necrosis factor (tnf)-α homologs on different chromosomes of zebrafish (Danio rerio) and medaka (Oryzias latipes)[J].Mar Genomics, 2014, 13:1-9. doi: 10.1016/j.margen.2013.10.004
[31]	GRAYFER L, WALSH J G, BELOSEVIC M.Characterization and functional analysis of goldfish (Carassius auratus L.) tumor necrosis factor-alpha[J].Dev Comp Immunol, 2008, 32(5):532-543. doi: 10.1016/j.dci.2007.09.009
[32]	LAING K J, WANG T, ZOU J, et al.Cloning and expression analysis of rainbow trout Oncorhynchus mykiss tumour necrosis factor-α[J].Eur J Biochem, 2001, 268(5):1315-1322. doi: 10.1046/j.1432-1327.2001.01996.x
[33]	GARCÍA-CASTILLO J, PELEGRÍN P, MULERO V, et al.Molecular cloning and expression analysis of tumor necrosis factor α from a marine fish reveal its constitutive expression and ubiquitous nature[J].Immunogenetics, 2002, 54(3):200-207. doi: 10.1007/s00251-002-0451-y
[34]	钱曦, 华雪铭, 黄旭雄, 等.异育银鲫c型溶菌酶全长cDNA序列的生物信息学分析及其组织表达分析[J].中国生物化学与分子生物学报, 2008, 24(4):337-347. http://cjbmb.bjmu.edu.cn/CN/abstract/abstract19063.shtml
[35]	MINAGAWA S, HIKIMA J, HIRONO I, et al.Expression of Japanese flounder c-type lysozyme cDNA in insect cells[J].Dev Comp Immunol, 2001, 25(5/6):439-445. https://www.sciencedirect.com/science/article/pii/S0145305X01000131
[36]	章琼, 孙盛明, 李冰, 等.团头鲂g型溶菌酶基因全长cDNA的克隆与表达分析[J].南方水产科学, 2015, 2(2):41-49. http://www.schinafish.cn/CN/abstract/abstract9202.shtml
[37]	杨勇. 日本鳗鲡的C-型和g-型溶菌酶基因及其功能研究[D]. 厦门: 集美大学, 2015: 42-43. http://cdmd.cnki.com.cn/Article/CDMD-10390-1015350253.htm
[38]	JIMÉNEZ-CANTIZANO R M, INFANTE C, MARTINANTONIO B, et al.Molecular characterization, phylogeny, and expression of c-type and g-type lysozymes in brill (Scophthalmus rhombus)[J].Fish Shellfish Immunol, 2008, 25(1/2):57-65. https://www.peertechz.com/Aquaculture-Fishery-Sciences/epub/IJAFS...
[39]	YE X, ZHANG L, TIAN Y, et al.Identification and expression analysis of the g-type and c-type lysozymes in grass carp Ctenopharyngodon idellus[J].Dev Comp Immunol, 2010, 34(5):501-509. doi: 10.1016/j.dci.2009.12.009
[40]	潘宝平, 宋欣, 罗凯娅, 等.青蛤(Cyclina sinensis)溶菌酶基因在鳗弧菌(Vibrio anguillarum)刺激下的表达[J].海洋与湖沼, 2010, 41(6):901-906. doi: 10.11693/hyhz201006016016
[41]	HIKIMA J, HIRONO I.Molecular cloning and novel repeated sequences of a C-type lysozyme gene in Japanese flounder (Paralichthys olivaceus)[J].Mar Biotechnol (NY), 2000, 2(3):241-247. doi: 10.1007/s101269900028
[42]	张士璀, 许娜.动物型溶菌酶研究新进展[J].中国海洋大学学报(自然科学版), 2014, 44(6):46-51. http://www.docin.com/p-1157415327.html
[43]	王美娟. 淇河鲫c型和g型溶菌酶基因克隆及表达分析[D]. 新乡: 河南师范大学, 2015: 46. http://cdmd.cnki.com.cn/Article/CDMD-10476-1015416434.htm

Tissue distribution and response to infection of Photobacterium damselae subsp. piscicida of Tf, TNFα and C-Lys in Trachinotus ovatus

Abstract

References

Related Articles

Catalog

Related

[1]	REN Xiaoyao, TANG Baojun, ZHENG Hanfeng, LIU Yujia, WEI Tao. Effects of acute hypoxia stress on respiratory metabolism and related gene expression of juvenile Babylonia areolate[J]. South China Fisheries Science, 2025, 21(2): 149-156. DOI: 10.12131/20240205
[2]	ZHAO Shuhao, ZHU Huaping, MA Dongmei, GENG Guohua, ZHONG Zaixuan, FAN Jiajia, TIAN Yuanyuan, LIU Xiande, HAN Fang. Relationship between diurnal feeding rhythm and agrp gene expression level in Cyprinus carpio rubrofuscus[J]. South China Fisheries Science, 2024, 20(3): 101-111. DOI: 10.12131/20240014
[3]	HE Liangyin, SHI Xiaoli, LIN Jiayang, HUANG Shuyi, HUANG Weiqing, HAN Kunhuang. Cloning of adsl gene in Larimichthys crocea and analysis of its correlation between expression level and inosine content[J]. South China Fisheries Science, 2024, 20(2): 111-118. DOI: 10.12131/20230178
[4]	ZHANG Wenzheng, YANG Li, YAO Junjie, MENG Qingmi, JI Da. Study on muscle fiber characteristics, metabolic enzymes and gene expression of caudal stalk of golden-backed carp (Cyprinus carpio var. Jinbei)[J]. South China Fisheries Science, 2023, 19(4): 77-85. DOI: 10.12131/20220320
[5]	GAO Jie, GUO Huayang, LIU Mingjian, LIU Baosuo, ZHU Kecheng, ZHANG Nan, GUO Liang, ZHANG Dianchang. Response of caspase genes to Streptococcus agalactiae in cells of spleen of golden pompano (Trachinotus ovatus)[J]. South China Fisheries Science, 2022, 18(6): 44-51. DOI: 10.12131/20220099
[6]	LIU Meiling, SHANGGUAN Xiaozhao, WANG Xiaoqiang, WANG Yayu, WANG Guiling, LI Jiale. Molecular characterization and expression of MAP2K1 gene in Hyriopsis cumingii[J]. South China Fisheries Science, 2022, 18(5): 91-99. DOI: 10.12131/20210328
[7]	LI Zhimin, QIAN Jiahui, LIU Jianyong, AI Jialin, TAN Keqin. Molecular cloning, expression of alpha-amylase gene of Haliotis diversicolor supertexta and correlation with growth traits[J]. South China Fisheries Science, 2017, 13(6): 14-21. DOI: 10.3969/j.issn.2095-0780.2017.06.002
[8]	YU Wenbo, ZHU Kecheng, GUO Huayang, ZHANG Nan, SUN Xiaoxiao, WU Na, ZHANG Dianchang. Cloning and expression analysis of MHCⅡβ gene in Trachinotus ovatus[J]. South China Fisheries Science, 2017, 13(4): 69-79. DOI: 10.3969/j.issn.2095-0780.2017.04.009
[9]	LI Yundong, ZHOU Falin, HUANG Jianhua, MA Zhenhua, YANG Qibin, QIU Lihua, FU Mingjun, CHEN Jinsong, JIANG Shigui. Cloning and expression analysis of Cathepsin L cDNA of Penaeus monodon[J]. South China Fisheries Science, 2016, 12(3): 58-66. DOI: 10.3969/j.issn.2095-0780.2016.03.008
[10]	WEN Jiufu, GAO Zexia, LUO Wei, TONG Jingou, WANG Weimin. Observation of gonad at different development stages and expression analysis of Kiss2/Kiss2r genes in Megalobrama amblycephala[J]. South China Fisheries Science, 2013, 9(3): 44-50. DOI: 10.3969/j.issn.2095-0780.2013.03.008

Tissue distribution and response to infection of Photobacterium damselae subsp. piscicida of Tf, TNFα and C-Lys in Trachinotus ovatus

Abstract

References

Related Articles

Catalog

Related

Export File

Citation

Format

Content