Volume 18 Issue 5
Oct.  2022
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LIU Meiling, SHANGGUAN Xiaozhao, WANG Xiaoqiang, WANG Yayu, WANG Guiling, LI Jiale. Molecular characterization and expression of MAP2K1 gene in Hyriopsis cumingii[J]. South China Fisheries Science, 2022, 18(5): 91-99. DOI: 10.12131/20210328
Citation: LIU Meiling, SHANGGUAN Xiaozhao, WANG Xiaoqiang, WANG Yayu, WANG Guiling, LI Jiale. Molecular characterization and expression of MAP2K1 gene in Hyriopsis cumingii[J]. South China Fisheries Science, 2022, 18(5): 91-99. DOI: 10.12131/20210328
shu

Molecular characterization and expression of MAP2K1 gene in Hyriopsis cumingii

  • 1.

    Shanghai Ocean University/Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China

  • 2.

    National Demonstration Center for Experimental Fisheries Science Education/Shanghai Engineering Research Center of Aquaculture, Shanghai 201306, China

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  • Received Date: November 02, 2021
  • Revised Date: January 12, 2022
  • Accepted Date: February 13, 2022
  • Available Online: March 02, 2022
    Graphical Abstract
    • Abstract
  • In order to study the role of the MAP2K1 (MEK1) gene in the sex determination of Hyriopsis cumingii, we applied RACE (Rapid-amplification of cDNA ends) method to clone the MAP2K1 gene sequence. We conducted a real-time fluorescence quantitative analysis to compare MAP2K1 gene in six tissues (Gonads, adductor muscle, hepatopancreas, gills, mantle, foot), gonads at early developmental stage (1−8 month old) and the 1−3 years' level of expression in male and female glands of H. cumingii. We determined the location of MAP2K1 gene in the gonads of 2-year-old H.cumingii by in situ hybridization. The results show that the ORF region of MAP2K1 gene was 1 194 bp in length and encoded 397 amino acids. MAP2K1 gene was highly expressed in the ovary; the expression level was the highest at 2 months of age at early developmental stage; the expression results from 1−3 years of age show that the expression of MAP2K1 gene in the ovary was higher than that in the spermatozoa for the same period (P<0.05). The in situ hybridization results show that the MAP2K1 gene had a significant hybridization signal in the oocytes and eggs of female H. cumingii. RNAi results show that the expression of the downstream gene MAP2K1 gene decreased by 82.31% in females and 73.60% in males after interfering with the upstream gene C-MOS gene of MAP2K1 gene. In conclusion, MAP2K1 gene may be involved in the ovarian development process and is a female-biased gene in H. cumingii, and C-MOS gene affects its expression.
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