ZHANG Wenzheng, YANG Li, YAO Junjie, MENG Qingmi, JI Da. Study on muscle fiber characteristics, metabolic enzymes and gene expression of caudal stalk of golden-backed carp (Cyprinus carpio var. Jinbei)[J]. South China Fisheries Science, 2023, 19(4): 77-85. DOI: 10.12131/20220320
Citation: ZHANG Wenzheng, YANG Li, YAO Junjie, MENG Qingmi, JI Da. Study on muscle fiber characteristics, metabolic enzymes and gene expression of caudal stalk of golden-backed carp (Cyprinus carpio var. Jinbei)[J]. South China Fisheries Science, 2023, 19(4): 77-85. DOI: 10.12131/20220320

Study on muscle fiber characteristics, metabolic enzymes and gene expression of caudal stalk of golden-backed carp (Cyprinus carpio var. Jinbei)

More Information
  • Received Date: December 21, 2022
  • Revised Date: February 11, 2023
  • Accepted Date: February 22, 2023
  • Available Online: February 28, 2023
  • Golden-backed carp (Cyprinus carpio var. Jinbei) is a common carp that has lived in rice fields for many generations through long-term natural and artificial selection. In order to promote its paddy field breeding model and provide data supports for the breeding of improved varieties by this model, we analyzed the caudal stalk muscle fibers, related metabolic enzymes and gene expressions by using histology, enzymology and molecular biology methods, and explored the adaptive characteristics of caudal stalk muscle growth under the shallow ecological conditions of rice field. The results show that the diameter of caudal stalk muscle fiber in paddy field group was larger than that in pond group at flowering stage and harvest stage with significant differences (P<0.05). The cross-section area of caudal stalk muscle fiber in paddy field group was larger than that in pond group at flowering stage and harvest stage, with significant differences at harvest stage (P<0.05), while the density of caudal stalk muscle fiber in paddy field group was smaller than that in pond group. There were significant differences at harvest stage (P<0.05). The activities of lactate dehydrogenase (LDH), catalase (CAT) and Ca2+-ATPase in metabolic enzymes were higher in paddy field group than in pond group at flowering stage and harvest stage. The expression level of peroxisome proliferation-activated receptor γ-coactivator α (PGC-1α) gene in paddy field group was significantly higher than that in pond group (P<0.05). The results reveal that the expression levels of adenylate activated protein kinase (AMPK) and silence-regulating factor 1 (SIRT1) gene in paddy field group were significantly lower than those in pond group at flowering stage and harvest stage (P<0.05). The resistance movement of the caudal stalk of golden-backed carp in rice field caused muscle hypertrophy, which was related with the activation of protein synthesis pathway, while its endurance exercise, was related with the mitochondrial biosynthesis. The higher PGC-1α expression level of golden-backed carp in rice flowering stage may be related with the increase of rice flowering, insects, biodiversity and biomass.
  • [1]
    周伟, 刘应聪. 云南绿汁江鱼类的形态特征及其适应意义[J]. 四川动物, 1999(1): 10-13.
    [2]
    沈伟良, 吴雄飞, 申屠基康, 等. 同饵料及养殖环境对大黄鱼(Larimichthys crocea)形态差异的影响[J]. 渔业科学进展, 2017, 38(6): 70-77.
    [3]
    孟庆闻, 缪学祖, 俞泰济. 鱼类学: 形态·分类[M]. 上海: 科学技术出版社, 1989: 8-14.
    [4]
    郭梁, 任伟征, 胡亮亮, 等. 传统稻鱼系统中“田鲤鱼”的形态特征[J]. 应用生态学报, 2017, 28(2): 665-672.
    [5]
    刘雨婷, 黄世玉, 李榴佳, 等. 池塘与稻田养殖鲤鱼生物学指数和肌肉营养价值的比较[J]. 中国农学通报, 2022, 38(4): 159-164.
    [6]
    MOODY E K, LOZANO-VILANO M L. Predation drives morphological convergence in the Gambusia panuco species group among lotic and lentic habitats[J]. J Evol Biol. 2018, 31(4): 491-501.
    [7]
    孙冰, 侯艳茹, 徐丽媛, 等. 不同饲养方式下苏尼特羊肌纤维组成和羊肉品质的比较研究[J]. 食品工业科技, 2022, 43(11): 96-103.
    [8]
    文超越, 段叶辉, 李颖慧, 等. 能量感应网络AMPK/SIRT1/PGC-lα对骨骼肌纤维类型转化调节[J]. 动物营养学报, 2016, 28(1): 57-63.
    [9]
    沈文清, 何标, 丁树哲. AMPK——运动调控骨骼肌糖脂代谢的重要激酶[J]. 生命科学, 2022, 34(6): 631-643.
    [10]
    CANTÓ C, AUWERX J. PGC-1alpha, SIRT1 and AMPK, an ener-gy sensing network that controls energy expenditure[J]. Curr Opin Lipidol, 2009, 20(2): 98-105. doi: 10.1097/MOL.0b013e328328d0a4
    [11]
    JI D, SU X, YAO J J, et al. Genetic diversity and genetic differentiation of populations of golden-backed carp (Cyprinus carpio var. Jinbei) in traditional rice fields in Guizhou, China[J]. Animals (Basel), 2022, 12(11): 1377.
    [12]
    闫冠杰, 曹振东, 彭姜岚, 等. 运动锻炼对鲤鱼幼鱼形态参数的影响[J]. 重庆师范大学学报(自然科学版), 2011, 28(3): 18-21.
    [13]
    纪达, 许劲松, 姚俊杰, 等. 贵州省5个金背鲤 (Cyprinus carpio var. Jinbei) 地理种群的遗传多样性与遗传结构分析[J]. 水产学杂志, 2022, 35(5): 8-17.
    [14]
    曾宪文, 余长生, 吴正武, 等. 水稻—金背鲤共生模式与技术[J]. 作物研究, 2022, 36(2): 176-179. doi: 10.16848/j.cnki.issn.1001-5280.2022.02.16
    [15]
    王伟, 杨又兵, 曾珍珍, 等. 两个黄河鲤鱼群体肌纤维特性及肌肉营养成分分析[J]. 黑龙江畜牧兽医, 2017(11): 239-243, 299. doi: 10.13881/j.cnki.hljxmsy.2017.1007
    [16]
    NISHIMURA A, SUGITA M, KATO K, et al. Hypoxia increases muscle hypertrophy induced by resistance training[J]. Int J Sports Physiol Perform, 2010, 5(4): 497-508. doi: 10.1123/ijspp.5.4.497
    [17]
    解长青. 肌纤维类型与运动训练[J]. 南京体育学院学报(自然科学版), 2012, 11(2): 34-35. doi: 10.15877/j.cnki.nsin.2012.02.004
    [18]
    李凤杰. 不同营养状态下南方鲇幼鱼大侧肌和心肌的适应性变化研究[D]. 重庆: 西南大学, 2012: 27-31.
    [19]
    YAN G J, HE X K, CAO Z D, et al. Effects of fasting and feeding on the fast-start swimming performance of southern catfish Silurus meridionalis[J]. J Fish Biol, 2015, 86(2): 605-614. doi: 10.1111/jfb.12595
    [20]
    乔云贵, 黄洪亮, 黄妙芬, 等. 不同淡水鱼类游泳速度的初步研究[J]. 湖南农业科学, 2012(15): 116-119. doi: 10.3969/j.issn.1006-060X.2012.15.038
    [21]
    朱峰磊, 张敬敬, 龙静, 等. 游泳加速模式对南方鲇和团头鲂运动后代谢恢复的影响[J]. 淡水渔业, 2020, 50(3): 65-71. doi: 10.3969/j.issn.1000-6907.2020.03.009
    [22]
    吕敏, 甘晖, 陈田聪, 等. 瓦氏黄颡鱼在稻田和池塘养殖中的生长性能和肌肉品质比较[J]. 水产学杂志, 2022, 35(1): 75-81. doi: 10.3969/j.issn.1005-3832.2022.01.013
    [23]
    明俊超, 董在杰, 梁政远, 等. 6个不同鲤群体的形态差异分析[J]. 广东海洋大学学报, 2009, 29(6): 1-6. doi: 10.3969/j.issn.1673-9159.2009.06.001
    [24]
    余科, 李长贵, 安苗, 等. 从江稻田鲤与国内7种鲤的形态学差异研究[J]. 贵州畜牧兽医, 2022, 46(3): 11-16.
    [25]
    谭春明, 赵旺, 马振华, 等. 红腹海参消化道指标、组织学和酶活性的季节变化[J]. 南方水产科学, 2022, 18(5): 39-45.
    [26]
    李丹丹, 陈丕茂, 朱爱意, 等. 黑鲷幼鱼力竭运动后代谢酶活性的恢复水平[J]. 南方水产科学, 2018, 14(6): 59-65.
    [27]
    GRUNOW B, STANGE K, BOCHERT R, et al. Histological and biochemical evaluation of skeletal muscle in the two salmonid species Coregonus maraena and Oncorhynchus mykiss[J]. PLoS One, 2021, 16(8): e0255062. doi: 10.1371/journal.pone.0255062
    [28]
    沈德功. 人体肌纤维类型与运动训练[J]. 西藏体育, 1996(1): 49-52.
    [29]
    刘明镜, 王志坚. 斑马鱼对无氧运动训练的适应性变化[J]. 动物学研究, 2013, 34(3): 190-195.
    [30]
    夏伟. 运动训练对鲤鱼 (Cyprinus carpio) 幼鱼游泳能力的影响及其代谢机制探讨[D]. 重庆: 重庆师范大学, 2012: 1-9.
    [31]
    宋洪建, 刘伟, 王继隆, 等. 锶对大麻哈鱼稚鱼生长发育及肌肉ATP酶活性的影响[J]. 水产学杂志, 2013, 26(4): 23-28.
    [32]
    殷中专, 田元勇, 徐昙烨, 等. 鱼贝类肌肉品质变化与能量代谢关联[J]. 水产学报, 2022, 46(7): 1143-1153.
    [33]
    MEIS L D. Energy interconversion by the sarcoplasmic reticulum Ca2+-ATPase: ATP hydrolysis, Ca2+ transport, ATP synthesis and heat production[J]. An Acad Bras Cienc, 2000, 72(3): 365-379. doi: 10.1590/S0001-37652000000300010
    [34]
    李春艳, 肖艳芬. 抗阻/耐力训练诱导骨骼肌分子响应的比较研究[J]. 湖北体育科技, 2010, 29(4): 427-429.
    [35]
    VISSING K, MCGEE S, FARUP J, et al. Differentiated mTOR but not AMPK signaling after strength vs endurance exercise in training-accustomed individuals[J]. Scand J Med Sci Sports, 2013, 23(3): 355-366. doi: 10.1111/j.1600-0838.2011.01395.x
    [36]
    田浩楠, 汪军, 张锋, 等. 有氧和力量同期训练对骨骼肌肥大的影响及其分子机制[J]. 中国体育科技, 2022, 58(10): 81-89.
    [37]
    SPAULDING H R, YAN Z. AMPK and the adaptation to exercise[J]. Annu Rev Physiol, 2022, 84: 209-227. doi: 10.1146/annurev-physiol-060721-095517
    [38]
    王宇, 袁倩, 王柏辉, 等. 腺苷酸活化蛋白激酶活性及其级联效应对肉品品质的影响研究进展[J]. 食品科学, 2018, 39(17): 298-304. doi: 10.7506/spkx1002-6630-201817047
    [39]
    BAAR K. Training for endurance and strength: lessons from cell signaling[J]. Med Sci Sports Exerc, 2006, 38(11): 1939-1944. doi: 10.1249/01.mss.0000233799.62153.19
    [40]
    CANTÓ C, GERHART-HINES Z, FEIGE J N, et al. AMPK regulates energy expenditure by modulating NAD+ metabolism and SIRT1 activity[J]. Nature, 2009, 458(7241): 1056-1060. doi: 10.1038/nature07813
    [41]
    RUDERMAN N B, XU X J, NELSON L, et al. AMPK and SIRT1: a long-standing partnership?[J]. Am J Physiol Endocrinol Metab, 2010, 298(4): 751-760. doi: 10.1152/ajpendo.00745.2009
  • Cited by

    Periodical cited type(2)

    1. 杨立,蒙庆米,白天泉,李嘉尧,姚俊杰,马兰. 海拔对稻田金背鲤肠道结构、消化酶活性和肠道菌群的影响. 华南农业大学学报. 2024(06): 898-907 .
    2. 马兰,蒙庆米,李嘉尧,秦国兵,姚俊杰,王秀龙,杨立. 稻田增加沟坑对金背鲤肠道结构、消化酶活性及微生物群落的影响. 华南农业大学学报. 2024(06): 889-897 .

    Other cited types(0)

Catalog

    Article views (391) PDF downloads (53) Cited by(2)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return