Volume 3 Issue 6
Dec.  2007
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LIU Kai, LU Hongda. Phagocytosis of haemocytes in Eriocheir sinensis in vitro[J]. South China Fisheries Science, 2007, 3(6): 47-51.
Citation: LIU Kai, LU Hongda. Phagocytosis of haemocytes in Eriocheir sinensis in vitro[J]. South China Fisheries Science, 2007, 3(6): 47-51.
shu

Phagocytosis of haemocytes in Eriocheir sinensis in vitro

  • 1.

    Fisheries Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

  • 2.

    College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China

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  • Received Date: July 08, 2007
  • Revised Date: August 04, 2007
    Graphical Abstract
    • Abstract

  • Phagocytosis, the cellular defensing reactions of Eriocheir sinensis, was studied in vitro using Bacillus cereus and formalinized mouse erythrocytes as test particles. The results showed that 100.0 mg·mL-1 L-cysteine van HARREVALD′s solution could anticoagulate hemolymph of E.sinensis. And phagocytosis was studied using a monolayer culture system which maintained cell viability at (92.5±0.9)% for 1hour at 20℃. Many of spread cells were phagocytic cells, which had pseudopodia and could phagocyte B.cereus in vitro. Uptake of the Gram-positive bacteria, B.cereus, was found to be (5.1±1.4)%. The formalinized mouse erythrocyte was not phagocytosed at all. Serum incubation did not increase the percentage of uptake of any of the test particles with the haemocytes.

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    • References (18)
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