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Dec.  2007
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LIU Kai, LU Hongda. Phagocytosis of haemocytes in Eriocheir sinensis in vitro[J]. South China Fisheries Science, 2007, 3(6): 47-51.
Citation: LIU Kai, LU Hongda. Phagocytosis of haemocytes in Eriocheir sinensis in vitro[J]. South China Fisheries Science, 2007, 3(6): 47-51.
shu

Phagocytosis of haemocytes in Eriocheir sinensis in vitro

  • 1.

    Fisheries Research Institute, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

  • 2.

    College of Aqua-life Science and Technology, Shanghai Fisheries University, Shanghai 200090, China

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  • Received Date: July 08, 2007
  • Revised Date: August 04, 2007
    Graphical Abstract
    • Abstract

  • Phagocytosis, the cellular defensing reactions of Eriocheir sinensis, was studied in vitro using Bacillus cereus and formalinized mouse erythrocytes as test particles. The results showed that 100.0 mg·mL-1 L-cysteine van HARREVALD′s solution could anticoagulate hemolymph of E.sinensis. And phagocytosis was studied using a monolayer culture system which maintained cell viability at (92.5±0.9)% for 1hour at 20℃. Many of spread cells were phagocytic cells, which had pseudopodia and could phagocyte B.cereus in vitro. Uptake of the Gram-positive bacteria, B.cereus, was found to be (5.1±1.4)%. The formalinized mouse erythrocyte was not phagocytosed at all. Serum incubation did not increase the percentage of uptake of any of the test particles with the haemocytes.

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    • References (18)
  • [1]
    陈昌福, 陈萱, 陈超然, 等. 水产甲壳动物的免疫防御机能及其免疫预防研究进展[J]. 华中农业大学学报, 2003, 22(2): 197-203. http://hnxbl.cnjournals.net/hznydxzr/ch/reader/view_abstract.aspx?file_no=20030245
    [2]
    VAN HARREVALD A. A physiological solution for freshwater crustaceans[J]. Proc Soc Exp Biol Med, 1936, 34(4): 428-432. doi: 10.3181/00379727-34-8647C
    [3]
    GUPTA J D, HARLEY J P. Use of formalinized sheep erythrocytes in the rubella hemagglutination-inhibition test[J]. Am Soc Microbiol, 1970, 20(5): 843-844. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC377059/
    [4]
    李光友, 王青. 中国对虾血细胞及其免疫研究[J]. 海洋与湖沼, 1995, 26(6): 591-597. http://www.cnki.com.cn/Article/CJFDTotal-HYFZ199506004.htm
    [5]
    张明辉, 陆宏达. 中华绒螯蟹血淋巴抗凝剂的筛选[J]. 水产科技情报, 2005, 32(6): 93-98. http://sckjqb.ijournals.cn/sckjqb/article/abstract/20050619
    [6]
    SMITH V J, RATCLIFFE N A. Host defence reactions of the shore crab, Carcinus maenas(L) in vitro[J]. J Mar Biol Assoc U K, 1978, 58(4): 367-379. doi: 10.1017/S0025315400028046
    [7]
    张卓然, 周正任, 陈绪林. 实用细胞培养技术[M]. 北京: 人民卫生出版社, 1999: 30. https://wenku.baidu.com/view/e7ebae8df5335a8103d220aa.html?fr=xueshu
    [8]
    MCKAY D, JENKIN C R. Immunity in the invertebrates Ⅱ: Adaptive immunity in the crayfish Parachaeraps bicarinatus[J]. Immunol, 1969, 17(1): 127-137. doi: 10.1038/icb.1969.12
    [9]
    MCKAY D, JENKIN C R. Immunity in the invertebrates: the role of serum factors in phagocytosis of erythrocytes by haemocytes of crayfish (Parachaeraps bicarinatus)[J]. Aust J Exp Biol Med Sci, 1970, 48(2): 139-150. doi: 10.1038/icb.1970.13
    [10]
    陆宏达. 中华绒螯蟹血细胞的显微、亚显微形态结构及其分类[J]. 水生生物学报, 2002, 26(5): 494-500. doi: 10.3321/j.issn:1000-3207.2002.05.012
    [11]
    HOSE J E, MARTIN G G. Defense functions of granulocytes in the ridgeback prawn Sicyonia ingentis[J]. J Invertebr Pathol, 1989, 53(3): 335-346. doi: 10.1016/0022-2011(89)90097-9
    [12]
    SODERHALL K, SMITH V J, JOHANSSON M W. Exocytosis and uptake of bacteria by isolated haemocyte population of two crustaceans: evidence for cellular cooperation in the defence reactions of arthropods[J]. Cell Tissue Res, 1986, 245(1): 43-49. doi: 10.1007/BF00218085
    [13]
    陆宏达, 张明辉. 中华绒螯蟹血细胞数及离体后形态学变化[J]. 水产学报, 2006, 30(4): 454-462. doi: 10.3321/j.issn:1000-0615.2006.04.004
    [14]
    PATERSON W D, STEWART J E, ZWICKER B M. Phagocytosis as a celluar immune response mechanism in the American lobster, Homarus americanus[J]. J Invertebr Pathol, 1976, 27(1): 95-104. doi: 10.1016/0022-2011(76)90033-1
    [15]
    杨军, 邱安东, 石安静. 圆背角无齿蚌血细胞吞噬作用的研究[J]. 动物学杂志, 1999, 34(6): 5-8. doi: 10.3969/j.issn.0250-3263.1999.06.002
    [16]
    VAN OSS C J, STINSON M W. Immunoglobulins as aspecific opsonins. Ⅰ. Influence of polyclonal and monoclonal immunoglobulins on the in vitro phagocytosis of latex particles and staphylococci by human neutrophils[J]. J Reticuloendothelial Soc, 1970, 8(5): 397-406. doi: 10.1007/s11783-011-0280-z
    [17]
    STINSON M W, VAN OSS C J. Immunoglobulins as aspecific opsonins. Ⅱ. The influence of specific and aspecific immunoglobulins on the in vitro phagocytosis of noncapsulated, capsulated and decapsulated bacteria by human neutrophils[J]. J Reticuloendothelial Soc, 1971, 9(5): 503-512. http://www.semanticscholar.org/paper/b2b348f2bd274b64d7eb6eb656165cb6754cb14d
    [18]
    TYSON C J, JENKIN C R. The importance of opsonic factors in the removal of bacteria from the circulation of the crayfish(Parachaeraps bicarinatus)[J]. Aust J Exp Biol Med Sci, 1973, 51(5): 609-615. doi: 10.1038/icb.1973.57
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