Volume 2 Issue 1
Feb.  2006
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WANG Xiaoyu, YU Dahui, GUO Yihui, HUANG Guiju, GONG Shiyuan. Diagnostic RAPD markers of seven Chinese pearl oyster species[J]. South China Fisheries Science, 2006, 2(1): 18-22.
Citation: WANG Xiaoyu, YU Dahui, GUO Yihui, HUANG Guiju, GONG Shiyuan. Diagnostic RAPD markers of seven Chinese pearl oyster species[J]. South China Fisheries Science, 2006, 2(1): 18-22.
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Diagnostic RAPD markers of seven Chinese pearl oyster species

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Fisheries College, Huazhong Agricultural University, Wuhan 430070, China

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  • Received Date: August 24, 2005
  • Revised Date: September 14, 2005
    Graphical Abstract
    • Abstract

  • Random amplified polymorphic DNA (RAPD) technique was employed in analyzing the specific genetic markers of seven pearl oysters including Pinctada maxima, P.margaritifera, P.nigra, P.albina, P.fucata, P.chemnitzi in the genus Pinctada and Pteria penguin in the genus Pteria. Twenty one primers of S series and OPM series were screened. Four primers produce 57 loci. The average number of loci amplified by each primer is 14.3. The mean number of fingerprints per species amplified by each primer is 4.9 with length varying from 250 to 2 000 bp. Primer S10 generate diagnostic markers for each species and can distinguish the seven species studied simultaneously. The other primers screened in this study can discriminate two or more species from the others.

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    • References (20)
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