Volume 2 Issue 1
Feb.  2006
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WANG Xiaoyu, YU Dahui, GUO Yihui, HUANG Guiju, GONG Shiyuan. Diagnostic RAPD markers of seven Chinese pearl oyster species[J]. South China Fisheries Science, 2006, 2(1): 18-22.
Citation: WANG Xiaoyu, YU Dahui, GUO Yihui, HUANG Guiju, GONG Shiyuan. Diagnostic RAPD markers of seven Chinese pearl oyster species[J]. South China Fisheries Science, 2006, 2(1): 18-22.
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Diagnostic RAPD markers of seven Chinese pearl oyster species

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Fisheries College, Huazhong Agricultural University, Wuhan 430070, China

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  • Received Date: August 24, 2005
  • Revised Date: September 14, 2005
    Graphical Abstract
    • Abstract

  • Random amplified polymorphic DNA (RAPD) technique was employed in analyzing the specific genetic markers of seven pearl oysters including Pinctada maxima, P.margaritifera, P.nigra, P.albina, P.fucata, P.chemnitzi in the genus Pinctada and Pteria penguin in the genus Pteria. Twenty one primers of S series and OPM series were screened. Four primers produce 57 loci. The average number of loci amplified by each primer is 14.3. The mean number of fingerprints per species amplified by each primer is 4.9 with length varying from 250 to 2 000 bp. Primer S10 generate diagnostic markers for each species and can distinguish the seven species studied simultaneously. The other primers screened in this study can discriminate two or more species from the others.

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    • References (20)
  • [1]
    王祯瑞. 中国近海珍珠贝科的研究[M]//中国科学院海洋研究所. 海洋科学集刊. 北京: 科学出版社, 1978, 14: 101-117.
    [2]
    王祯瑞. 软体动物门双壳纲珍珠贝亚目. 中国动物志, 无脊椎动物, 第31卷[M]. 北京: 科学出版社, 2002: 68-98.
    [3]
    HYND J S. A revision of the Australian pearl-shells, genus Pinctada(Lamellibranchia)[J ]. Aust J Mar Fresh Res, 1955, 6(1): 98-137. doi: 10.1071/MF9550098
    [4]
    李刚, 姜卫国, 魏贻尧. 合浦珠母贝、长耳珠母贝和大珠母贝种间人工杂交的研究: III同工酶谱的比较研究[J]. 热带海洋, 1983, 2(4): 321-328. https://xueshu.baidu.com/usercenter/paper/show?paperid=d25002588c7ad1d2cb78cdfe7ecc996a&site=xueshu_se&hitarticle=1
    [5]
    WILLIANS J G, KUBELIK A R, LIVAK K J, et al. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers[J]. Nucleic Acids Res, 1990, 18(22): 6531-6535. doi: 10.1093/nar/18.22.6531
    [6]
    WELSH J, MICHAEL M. Fingerprinting genomes using PCR with arbitrary primers[J]. Nucleic Acids Res, 1990, 18(24): 7213-7218. doi: 10.1093/nar/18.24.7213
    [7]
    邓怀, 张四明, 汪登强, 等. 十种常见淡水鱼类的RAPD鉴定[J]. 淡水渔业, 1998, 28(1): 8-10. doi: 10.3969/j.issn.1000-6907.1998.01.002
    [8]
    刘萍, 孟宪红, 庄志猛, 等. 3种对虾种间RAPD遗传标记[J]. 海洋水产研究, 2002, 23(2): 1-6. doi: 10.3969/j.issn.1000-7075.2002.02.001
    [9]
    LIU Z, LI P, ARGUE B J, et al. Inheritance of RAPD markers in channel catfish (Ictalurus punctatus), blue catfish (I. furcatus), and their F1, F2 and backcross hybrids[J]. Animal Genetics, 1998, 29(1): 58-62. doi: 10.1046/j.1365-2052.1998.00284.x
    [10]
    CROSSLAND S, COATES D, GRAHAME J, et al. Use of random amplified polymorphic DNAs (RAPDs) in separating two sibling species of Littorina[J]. Mar Ecol Prog Ser, 1993, 96(3): 301-305. doi: 10.3354/meps096301
    [11]
    KLINBUNGA S, AMPAYUP P, TASSANAKAJON A, et al. Development of species specific markers of the tropical oyster (Crassostrea belcheri) in Thailand[J]. Mar Biotechnol, 2000, 2(5): 476-484. doi: 10.1007/s101260000028
    [12]
    张四明, 邓怀, 汪登强, 等. 7种鲟形目鱼类亲缘关系的随机扩增多态性DNA研究[J]. 自然科学进展, 1999, 9(9): 818-823. doi: 10.3321/j.issn:1002-008X.1999.09.009
    [13]
    邹曙明, 楼允东, 孙效文, 等. 用RAPD方法研究草鱼、柏氏鲤和3个地理种群鲤的亲缘关系[J]. 中国水产科学, 2000, 7(3): 6-11. doi: 10.3321/j.issn:1005-8737.2000.01.002
    [14]
    夏德全, 曹萤, 吴婷婷, 等. 用RAPD方法分析太湖大银鱼、太湖新银鱼和寡齿新银鱼的亲缘关系[J]. 中国水产科学, 2000, 7(1): 12-15. doi: 10.3321/j.issn:1005-8737.2000.01.003
    [15]
    LIU Z J, LI P, ARGUE B J, et al. Random amplified polymorphic DNA markers: usefulness for gene mapping and analysis of genetic variation of catfish[J]. Aquac, 1999, 174(1): 59-68. doi: 10.1016/S0044-8486(99)00007-1
    [16]
    KLINBUNGA S, AMPAYUP P, TASSANAKAJON A, et al. Genetic diversity and molecular markers of cupped oysters (genera Crassostrea, Caccostrea, and Striostrea) in Thailand revealed by randomly amplified polymorphic DNA analysis[J]. Mar Biotechnol, 2001, 3(2): 133-144. doi: 10.1007/s101260000057
    [17]
    孙效文, 梁利群. 鲤鱼的遗传连锁图谱(初报)[J]. 中国水产科学, 2000, 7(3): 2-5.
    [18]
    阎冰, 叶力, 邓凤娇, 等. 马氏珠母贝与解氏珠母贝的随机扩增多态DNA分析[J]. 广西科学, 2001, 8(4): 287-290. doi: 10.3969/j.issn.1005-9164.2001.04.013
    [19]
    喻达辉, 朱嘉濠. 珠母贝属6个种的ITS2分子标记研究[J]. 南方水产, 2005, 1(4): 6-12. doi: 10.3969/j.issn.2095-0780.2005.04.002
    [20]
    LIU Z J, CORDES J F. DNA marker technologies and their applications in aquaculture genetics[J]. Aquac, 2004, 238(1): 1-37. doi: 10.1016/j.aquaculture.2004.05.027
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