LIANG Ying, JIN Yue-mei, TIAN Chuan-yuan. Effects of phosphorus restriction and supplement on the chlorophyll fluorescent parameters of Chlorella sp.[J]. South China Fisheries Science, 2008, 4(4): 1-7.
Citation: LIANG Ying, JIN Yue-mei, TIAN Chuan-yuan. Effects of phosphorus restriction and supplement on the chlorophyll fluorescent parameters of Chlorella sp.[J]. South China Fisheries Science, 2008, 4(4): 1-7.

Effects of phosphorus restriction and supplement on the chlorophyll fluorescent parameters of Chlorella sp.

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  • Received Date: April 02, 2008
  • Revised Date: April 28, 2008
  • The effects of different P concentrations(0, 10, 36.3, 290.4 μM)on the chlorophyll fluorescence parameters, cell density and relative chlorophyll content of Chlorella sp. were reported.The algae were obtained from the Microalgae Culture Center (MACC), Ocean University of China.The cultures were grown at 20±1℃, the salinity was adjusted to 31 by using distilled water, and the light intensity was 100 μmol · m-2 · s-1.One-way analysis of variance showed that P concentrations have significant effects on the photosynthesis and growth of Chlorella sp.with the culture age increasing(P < 0.05). For Chlorella sp., Fv/Fm, ΦPSII, ETR, qP, cell densities and chlorophyll contents all increased with the increase of P concentrations and reached their highest values at 36.3 μM.Multiple comparison tests showed that the optimal P concentrations for the photosynthesis and growth of Chlorella sp. is 36.3 μM.The chlorophyll fluorescence parameters showed characteristic NIFT responses following PO43- addition to P-starved Chlorella sp., fluorescence decreased but qP, NPQ increased steadily, ΦPSII increased sharply, and then a decrease to the same steady state as that prior to addition.Seven days later, the lacked nutrient P was added and chlorophyll fluorescence parameters were measured every 2 hours.The results showed that Fv/Fm, ΦPSII, ETR of Chlorella sp.were all recovered in 24 hours.The changes of qN, NPQ were not significant.

  • [1]
    潘晓华, 石庆华, 郭进耀, 等. 无机磷对植物叶片光合作用的影响及其机理的研究进展[J]. 植物营养与肥料学报, 1997, 3(3): 201-208. doi: 10.11674/zwyf.1997.0302
    [2]
    李绍长. 低磷胁迫对植物光合和呼吸作用的影响[J]. 石河子大学学报, 2003, 7(2): 157-160. doi: 10.3969/j.issn.1007-7383.2003.02.024
    [3]
    李鹏民, 高辉远, STRASSER R J. 快速叶绿素荧光诱导动力学分析在光合作用研究中的应用[J]. 植物生理与分子生物学学报, 2005, 31(6): 559-566. doi: 10.3321/j.issn:1671-3877.2005.06.001
    [4]
    梁英, 尹翠玲, 冯力霞, 等. 微藻营养盐限制的检测方法[J]. 中国水产科学, 2005, 12(5): 661-667. doi: 10.3321/j.issn:1005-8737.2005.05.022
    [5]
    BEARDALL J, YOUNG E, ROBERTS S. Approaches for determining phytoplankton nutrient limitation[J]. Aquat Sci, 2001, 63(1): 44-69. doi: 10.1007/PL00001344
    [6]
    陈学豪, 吴钟强. 小球藻营养液投喂轮虫效果研究[J]. 福建农业学报, 2005, 20(增刊): 21-24. doi: 10.3969/j.issn.1008-0384.2005.z1.005
    [7]
    DAMALL D W. Selective recovery of gold and other metal irons from an algal biomass[J]. Environ Sci Technol, 1986, 20(2): 206-208. doi: 10.1021/es00144a018
    [8]
    张振华, 韩士群, 严少华, 等. 虾池接种小球藻对浮游生物及水化学环境的影响[J]. 水产科技情报, 2000, 27(2): 67-69. https://xueshu.baidu.com/usercenter/paper/show?paperid=a4108aa89e0607afa469f77216293042&site=xueshu_se&hitarticle=1
    [9]
    LIPPEMEIER S, HARTIG P, COLIJN F. Direct impact of silicate on the photosynthetic performance of the diatom Thalassiosira weissflogii assessed by on-and off-line PAM fluorescence measurements[J]. J Plankton Res, 1999, 21(2): 269-283. doi: 10.1093/plankt/21.2.269
    [10]
    YOUNG E B, BEARDALL J. Rapid ammonium-and nitrate-induced perturbations to chl-a fluorescence in nitrogen-stressed Dunaliella tertiolecta (Chlorophyta)[J]. J Phycol, 2003, 39(2): 332-342. doi: 10.1046/j.1529-8817.2003.02109.x
    [11]
    LIPPEMEIER S, FRAMPTON D M F, BLACKBURN S I, et al. Influence of phosphorus limitation on toxicity and photosynthesis of Alexandrium minutum (Dinophyceae) monitored by in-line detection of variable chlorophyll fluorescence[J]. J Phycol, 2003, 39(2): 320-331. doi: 10.1046/j.1529-8817.2003.01019.x
    [12]
    BEARDALL J, BERMAN T, HERAUD P, et al. A comparison of methods for detection of phosphate limitation in microalgae[J]. Aquat Sci, 2001, 63(1): 107-121. doi: 10.1007/PL00001342
    [13]
    YOUNG E B, BEARDALL J. Photosynthetic function in Dunaliella tertiolecta (Chlorophyta) during a nitrogen starvation and recovery cycle[J]. J Phycol, 2003, 39(5): 897-905. doi: 10.1046/j.1529-8817.2003.03042.x
    [14]
    SHELLY K, HERAUD P, BEARDALL J. Nitrogen limitation in Dunaliella tertiolecta (chlorophyceae) leads to increased susceptibility to damage by ultraviolet-B radiation but also increased repair capacity[J]. J Phycol, 2002, 38(4): 713-720. doi: 10.1046/j.1529-8817.2002.01147.x
    [15]
    GUILLARD R R, RYTHER J H. Studies of marine planktonic diatoms. I. Cyclotella nana Hustedt and Detonula confervacea (Cleve) Gran[J]. Can J Microbiol, 1962, 8: 229-239. doi: 10.1139/m62-029
    [16]
    ROBERTS S C. Physiological effects of phosphorus limitation on photosynthesis in two green algae[D]. Australia: Monash University, 1998: 116.
    [17]
    梁英, 冯力霞, 田传远, 等. 盐胁迫对塔胞藻生长及叶绿素荧光动力学的影响[J]. 中国海洋大学学报, 2006, 36(5): 726-732. doi: 10.3969/j.issn.1672-5174.2006.05.011
    [18]
    HOLLAND D, ROBERT S, BEARDALL J. Assessment of the nutrient status of phytoplankton: a comparison between conventional bioassays and nutrient-induced fluorescence transients (NIFTs)[J]. Ecol Ind, 2004, 4(3): 149-159. doi: 10.1016/j.ecolind.2004.04.001

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