Volume 8 Issue 6
Dec.  2012
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HUANG Guoqiang, LI Jie, TANG Xia, ZHANG Lingyan. Changes of GH, IGF-I, RNA/DNA ratio and glycogen content of juvenile brown flounder during thermal stress and restoration[J]. South China Fisheries Science, 2012, 8(6): 16-21. DOI: 10.3969/j.issn.2095-0780.2012.06.003
Citation: HUANG Guoqiang, LI Jie, TANG Xia, ZHANG Lingyan. Changes of GH, IGF-I, RNA/DNA ratio and glycogen content of juvenile brown flounder during thermal stress and restoration[J]. South China Fisheries Science, 2012, 8(6): 16-21. DOI: 10.3969/j.issn.2095-0780.2012.06.003
shu

Changes of GH, IGF-I, RNA/DNA ratio and glycogen content of juvenile brown flounder during thermal stress and restoration

  • 1.

    Guangxi Key Lab. of Marine Biotechnology, Guangxi Institute of Oceanology, Beihai 536000, China

  • 2.

    Fisheries College, Ocean University of China, Qingdao 266003, China

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  • Received Date: May 24, 2012
  • Revised Date: July 09, 2012
    Graphical Abstract
    • Abstract

  • To investigate the changes of GH, IGF-I, RNA/DNA ratio and glycogen content of brown flounder (Paralichthys olivaceus) to thermal manipulation, we cultured the juveniles at 8.5 ℃(T8.5), 13.0 ℃(T13.0), 17.5 ℃(T17.5), 22.0 ℃ (T22.0) and 26.5 ℃ (T26.5) for 10 d, and then adjusted to 22.0 ℃ for 30 d. The plasma IGF-I concentration of T17.5 was lower than that of T22.0 during stress period, but no significant difference was found in plasma GH and IGF-I concentration at different temperatures during stress and restoration periods. RNA/DNA ratios were significantly different in liver during 20~40 d but not significantly different in muscle during the whole experimental period. The glycogen contents in liver of T8.5, T13.0 and T26.5 were significantly lower than that of T17.5. At the end of the experiment, the glycogen contents in liver of T8.5, T13.0, T17.5 and T26.5 were slightly higher than that of T22.0. The glycogen contents in muscle of T26.5 and T13.0 were the highest at the end of stress period and on 20th day, repectively. The slow growth caused by 10-day culture at different temperatures could be completely compensated in 30 d after the temperature was adjusted to 22.0 ℃. No significant correlation was detected among plasma GH, IGF-I concentration, RNA/DNA ratio and growth rate.

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