Volume 11 Issue 6
Dec.  2015
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ZHAO Donghao, LI Zhiguang, WANG Xufeng, WANG Qiang, LI Yongxian, HUANG Ke, LI Liudong. Optimization of determination of nitrofuran metabolites in aquatic products by liquid chromatography tandem mass spectrometry[J]. South China Fisheries Science, 2015, 11(6): 58-64. DOI: 10.3969/j.issn.2095-0780.2015.06.008
Citation: ZHAO Donghao, LI Zhiguang, WANG Xufeng, WANG Qiang, LI Yongxian, HUANG Ke, LI Liudong. Optimization of determination of nitrofuran metabolites in aquatic products by liquid chromatography tandem mass spectrometry[J]. South China Fisheries Science, 2015, 11(6): 58-64. DOI: 10.3969/j.issn.2095-0780.2015.06.008
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Optimization of determination of nitrofuran metabolites in aquatic products by liquid chromatography tandem mass spectrometry

  • Key Lab.of Aquatic Product Processing, Ministry of Agriculture; Lab. of Quality & Safety Risky Assessment for Aquatic Product on Storage and Preservation (Guangzhou); Fishery Environment and Aquatic Products Quality Inspection & Testing Center of the Ministry of Agriculture (Guangzhou); South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

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  • Received Date: January 18, 2015
  • Revised Date: February 14, 2015
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  • The nitrofuran metabolites (AMOZ, SEM, AHD and AOZ) were determined by liquid chromatography tandem mass spectrometry (LC-MS/MS) in aquatic products. The sample pretreatment method and chromatographic conditions were optimized. The sample pH value was adjusted to 7.0~7.5 after derivation. Then the analytes were extracted by 8 mL ethyl acetate. Following centrifugation, the supernatant was transferred and evaporated to dryness under a gentle stream of nitrogen at 40 ℃. The residues were redissolved in 1 mL mobile phase of acetonitrile and 0.1% formic acid (5/95, V/V), and then centrifuged at 20 000×g for 10 min at 4 ℃. Gradient elution for LC separation was performed by using acetonitrile and 0.1% formic acid in water solution, and four nitrofuran metabolites were excellently separated with a C18 column. This accurate optimized method can promote efficiency greatly and is suitable for lot determination of nitrofuran metabolites in aquatic products.

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