Volume 8 Issue 4
Aug.  2012
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WU Yanyan, LIU Fajia, LI Laihao, YANG Xianqing, DENG Jianchao, CHEN Shengjun, MA Haixia. Determination and optimization of N-nitrosamines in salted fish by gas chromatography-mass spectrometry[J]. South China Fisheries Science, 2012, 8(4): 16-22. DOI: 10.3969/j.issn.2095-0780.2012.04.003
Citation: WU Yanyan, LIU Fajia, LI Laihao, YANG Xianqing, DENG Jianchao, CHEN Shengjun, MA Haixia. Determination and optimization of N-nitrosamines in salted fish by gas chromatography-mass spectrometry[J]. South China Fisheries Science, 2012, 8(4): 16-22. DOI: 10.3969/j.issn.2095-0780.2012.04.003
shu

Determination and optimization of N-nitrosamines in salted fish by gas chromatography-mass spectrometry

  • 1.

    Key Lab. of Aquatic Product Processing, Ministry of Agriculture; National R & D Center for Aquatic Product Processing; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Guangdong Ocean University, Zhanjiang 524088, China

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  • Received Date: February 14, 2012
  • Revised Date: March 11, 2012
    Graphical Abstract
    • Abstract

  • We determined N-nitrosamines in salted fish by gas chromatography-mass spectrometry (GC-MS), optimized the sample preparation conditions, compared the response intensity of N-nitrosamines by SPME and dichloromethane ultrasonic extraction as well as explored the impact of organic solvent, extraction time and times on the determination. By ion selective electrode method, we determined the contents of N-dimethylnitrosamine, N-diethylnitrosamine, N-nitroso-pyrrolidine and N-nitrosamines. Results show that the linear correlation coefficient is 0.999 2, 0.999 1, 0.999 1 and 0.999 2, respectively, with a linear range of 0~10 μg·mL-1. The method has good reproducibility (RSD≤2.1%, blank spiked recovery is 70%~80%) and high sensitivity (detection limit is 0.038 6 μg·kg-1, 0.022 7 μg·kg-1, 0.031 6 μg·kg-1 and 0.047 8 μg·kg-1, respectively).

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