Volume 8 Issue 2
Apr.  2012
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SUN Fengyu, SONG Zhongkui, ZHAO Peng, NIE Zhenping, SU Qiong, WANG Fangyu. RAPD analysis of genetic diversity of Scylla paramamosain population in Guangxi and neighboring sea areas[J]. South China Fisheries Science, 2012, 8(2): 30-35. DOI: 10.3969/j.issn.2095-0780.2012.02.005
Citation: SUN Fengyu, SONG Zhongkui, ZHAO Peng, NIE Zhenping, SU Qiong, WANG Fangyu. RAPD analysis of genetic diversity of Scylla paramamosain population in Guangxi and neighboring sea areas[J]. South China Fisheries Science, 2012, 8(2): 30-35. DOI: 10.3969/j.issn.2095-0780.2012.02.005
shu

RAPD analysis of genetic diversity of Scylla paramamosain population in Guangxi and neighboring sea areas

  • 1.

    Pharmacy and Life Science, South China University, Hengyang 421001, China

  • 2.

    Guangxi Key Lab. for Marine Biotechnology, Guangxi Institute of Oceanology, Beihai 536000, China

  • 3.

    Department of Life Science, Hengyang Normal University, Hengyang 421008, China

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  • Received Date: December 08, 2011
  • Revised Date: January 17, 2012
    Graphical Abstract
    • Abstract

  • Random amplified polymorphic DNA (RAPD) technology was employed to investigate the genetic diversity and structure of 6 Scylla paramamosain populations in Guangxi and its neighboring sea areas. We amplified a total of 99 individuals by using 8 random primers with 10 bp in length, and analyzed 44 valid loci, among which 31 loci are disclosed as polymorphism. The percentage of polymorphic locus (PPL) at species level is 70.45%. According to POPGENE analysis, the PPL in the 6 populations ranges from 29.55% to 54.55% with an average of 36.97%. The abundance of genetic diversity is QZW>DJ>ZZW>ZK>QH>LSW. Variance within populations is higher than that among populations, and genetic differentiation among populations reaches higher level. An analysis of molecular variance (AMOVA) shows that variance within populations accounts for 87.03%, and that among populations accounts for 12.97%. However, genetic differentiation among populations only reaches medium level. Mantel test shows that no significant correlation between genetic and geographic distance is detected in the 6 S.paramamosain populations. Cluster analysis indicates that the distribution of these populations displays no apparent geographic pattern.

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    • References (22)
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