Volume 6 Issue 6
Dec.  2010
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MO Yanxiu, WANG Xiaoqing, MO Yongliang. RAPD analysis of genetic diversity of Leiocassis longirostris[J]. South China Fisheries Science, 2010, 6(6): 77-80. DOI: 10.3969/j.issn.1673-2227.2010.06.014
Citation: MO Yanxiu, WANG Xiaoqing, MO Yongliang. RAPD analysis of genetic diversity of Leiocassis longirostris[J]. South China Fisheries Science, 2010, 6(6): 77-80. DOI: 10.3969/j.issn.1673-2227.2010.06.014
shu

RAPD analysis of genetic diversity of Leiocassis longirostris

  • 1.

    Department of Histology and Embryology, Xiangnan University, Chenzhou 423043, China

  • 2.

    College of Animal Science and Technology, Hunan Agricultural University, Changsha 410128, China

  • 3.

    Affiliated High School of Peking University Guangzhou Experimental School, Guangzhou 510250, China

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  • Received Date: May 06, 2010
  • Revised Date: June 11, 2010
    Graphical Abstract
    • Abstract

  • Taking wild Leiocassis longirostris from Dongting Lake and original breeding farm as samples and with 20 random primers, we conducted a RAPD analysis of genetic diversity of 10 wild individuals, finding 103 bands whose fragment size are 0.2~3.0 kb, and that each primer generates 2~9 bands with a polymorphic loci proportion of 41.75%.Genetic similarity coefficient and genetic distance within 2 populations are as follows: genetic similarity index(S) of Dongting Lake individuals varies from 0.879 5 to 0.983 3 and that of original breeding farm ones varies from 0.879 4 to 0.989 2;genetic distance(D) varies from 0.016 7 to 0.120 5 and that of original breeding farm ones varies from 0.031 9 to 0.110 8;the genetic similarity coefficient of 2 populations is 0.798 3~0.999 4 and the genetic distance is 0.016 7~0.301 7;Nei genetic diversity index is 0.326 9.The results indicate that L.longirostris has relatively high genetic diversity within populations.

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