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QU Youjun, WU Yong, LI Jiaer, LIU Chuwu. RAPD analysis of genetic difference among five species of grouper[J]. South China Fisheries Science, 2008, 4(2): 56-62.
Citation: QU Youjun, WU Yong, LI Jiaer, LIU Chuwu. RAPD analysis of genetic difference among five species of grouper[J]. South China Fisheries Science, 2008, 4(2): 56-62.
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RAPD analysis of genetic difference among five species of grouper

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    Fisheries College, Guangdong Ocean University, Zhanjiang 524025, China

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  • Received Date: December 16, 2007
  • Revised Date: December 28, 2007
    Graphical Abstract
    • Abstract

  • Genetic diversity and molecular evolution of five species of grouper, viz.Epinephelus lanceolatus, Cromileptes altivelis, E.fuscoguttatus, E.coioides and E.fario, were detected using RAPD technique. A total 120 random primers were evaluated for the usefulness. And thereinto, 19 random primers were selected according to the effect of PCR of genomic DNA of 5 species of grouper. The results showed as the followings: the polymorphic loci ratio in E.lanceolatus, C.altivelis, E.fuscoguttatus, E.coioides and E.fario (P) were 64.23%, 72.61%, 60.34%, 69.97% and 73.94%, respectively. The mean genetic similarity index of intra-species (S) were 0.8238, 0.8110, 0.8345, 0.8277, 0.8064, respectively, the mean genetic distances of intra-species (D) were 0.1762, 0.1890, 0.1655, 0.1723 and 0.1936, respectively, Nei′s gene diversity (H) were 0.1189, 0.1364, 0.1028, 0.1439 and 0.1648, respectively, and Shannon′s information index (Hi) were 0.1801, 0.1992, 0.1530, 0.2100, 0.2434, respectively. The mean genetic distances of inter-species (Dxy) were between 0.3964~0.6085.

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