JIANG Miao, YANG Xianqing, LI Laihao, CEN Jianwei, HAO Shuxian, ZHANG Chaohua. Application of endogenous enzymes in astaxanthin extraction from shrimp shell[J]. South China Fisheries Science, 2011, 7(2): 55-60. DOI: 10.3969/j.issn.2095-0780.2011.02.009
Citation: JIANG Miao, YANG Xianqing, LI Laihao, CEN Jianwei, HAO Shuxian, ZHANG Chaohua. Application of endogenous enzymes in astaxanthin extraction from shrimp shell[J]. South China Fisheries Science, 2011, 7(2): 55-60. DOI: 10.3969/j.issn.2095-0780.2011.02.009

Application of endogenous enzymes in astaxanthin extraction from shrimp shell

More Information
  • Received Date: September 15, 2010
  • Revised Date: November 25, 2010
  • The extraction of astaxanthin from shrimp shell with endogenous enzyme is studied, and astaxanthin content in the extract is determined by high performance liquid chromatography (HPLC).The pH, temperature and hydrolysis time are considered as the factors to study the extraction effect. Orthogonal test is adopted for optimization, taking the yield of astaxanthin from shrimp shell as the evaluation indicator. The results show that the optimum process conditions are 4.0 pH, 50 ℃ and 1.5 h hydrolysis time, with the astaxanthin extraction from wet shrimp shell of 32.16 μg·g-1, which is 28% more than the extraction by direct ultrasonic treatment. No significant effect on the yield of astaxanthin is observed by adding lipase. Extracting astaxanthin by endogenous enzymes achieves better extraction effect and is economical.

  • [1]
    GOTO S, KOGURE K, ABE K, et al. Efficient radical trapping at the surface and inside the phospholipid membrane is responsible for highly potent antiperoxidative activity of the carotenoid astaxanthin[J]. Biochim Biophys Acta, 2001, 1512(2): 251-258. doi: 10.1016/S0005-2736(01)00326-1
    [2]
    NAGUIB Y M A. Antioxidant activities of astaxanthin and related carotenoids[J]. J Agric Food Chem, 2000, 48(4): 1150-1154. doi: 10.1021/jf991106k
    [3]
    SAVOUREN, BRIAND G, AMORYTMC, et al. Vitamin A status and metabolism of cutaneous polyamines in the hair-less mouse after UV irradiation: action of beta-carotene and astaxanthin[J]. Int J Vitam Nutr Res, 1995, 65(2): 79-86. https://pubmed.ncbi.nlm.nih.gov/7591536/
    [4]
    魏东, 严小君. 天然虾青素的超级抗氧化活性及其应用[J]. 中国海洋药物, 2001, 20(4): 45-50. doi: 10.3969/j.issn.1002-3461.2001.04.011

    WEI Dong, YAN Xiaojun. Super-antioxidant activity of natural astaxanthin and its application[J]. China J Mar Med, 2001, 20(4): 45-50. (in Chinese) doi: 10.3969/j.issn.1002-3461.2001.04.011
    [5]
    李艳艳, 翟占军, 张银花, 等. 虾青素在水产养殖上的应用[J]. 北京农学院学报, 2009, 24(2): 78-80. doi: 10.3969/j.issn.1002-3186.2009.02.021

    LI Yanyan, ZHAI Zhanjun, ZHANG Yinhua, et al. Application in aquatic animal of astaxanthin[J]. J Beijing Univ Agric, 2009, 24(2): 78-80. (in Chinese) doi: 10.3969/j.issn.1002-3186.2009.02.021
    [6]
    彭光华, 李忠, 张声华, 等. 6种类胡萝卜素对人乳腺癌细胞株细胞间隙连接通讯功能影响的比较[J]. 食品科学, 2008, 29(6): 45-48. doi: 10.3321/j.issn:1002-6630.2008.06.003

    PENG Guanghua, LI Zhong, ZHANG Shenghua, et al. Comparison of effect of six kinds of carotenoids on gap junctional intercellular communication of human breast cancer cell[J]. Food Sci, 2008, 29(6): 45-48. (in Chinese) doi: 10.3321/j.issn:1002-6630.2008.06.003
    [7]
    刘宏超, 杨丹. 从虾壳中提取虾青素工艺及其生物活性应用研究进展[J]. 化学试剂, 2009, 31(2), 105-108. doi: 10.3969/j.issn.0258-3283.2009.02.007

    LIU Hongchao, YANG Dan. Progress in research on extracting astaxanthin from shrimp shell and application of its biological activity[J]. Chem Reagent, 2009, 31(2), 105-108. (in Chinese) doi: 10.3969/j.issn.0258-3283.2009.02.007
    [8]
    焦雪峰. 虾青素在化妆品中的应用[J]. 广东化工, 2006, 33(1): 13-15. doi: 10.3969/j.issn.1007-1865.2006.01.007

    JIAO Xuefeng. Application of astaxanthin on cosmetics[J]. Guangdong Chem Ind, 2006, 33(1): 13-15. (in Chinese) doi: 10.3969/j.issn.1007-1865.2006.01.007
    [9]
    陆开形, 蒋霞敏, 翟兴文. 虾青素的生物学功能及应用[J]. 宁波大学学报, 2006, 16 (1): 95-98. doi: 10.3969/j.issn.1001-5132.2003.01.021

    LU Kaixing, JIANG Xiamin, ZHAI Xingwen. Biological function and application of astaxanthin[J]. J Ningbo Univ, 2006, 16(1): 95-98. (in Chinese) doi: 10.3969/j.issn.1001-5132.2003.01.021
    [10]
    胡雅馨, 惠伯棣, 李阳. 食用虾中虾青素类化合物的含量和组成[J]. 食品科学, 2007, 28, (8): 389-392. doi: 10.3321/j.issn:1002-6630.2007.08.094

    HU Yaxin, HUI Bodi, LI Yang. Content and composition of astaxanthin related compounds in shrimp[J]. Food Sci, 2007, 28(8): 389-392. (in Chinese) doi: 10.3321/j.issn:1002-6630.2007.08.094
    [11]
    何秋生, 李向阳, 方振华, 等. 淡水鱼的加工及综合利用初探[J]. 中国水产, 1999(7): 44-46.

    HE Qiusheng, LI Xiangyang, FANG Zhenhua, et al. The first exploration on processing and comprehensive unilization of freshwater fish[J]. China Fish, 1999(7): 44-46. (in Chinese)
    [12]
    顾晨光, 王建军. 虾脑酱的制作[J]. 食品科学, 1991(7): 23-24.

    GU Chenguang, WANG Jianjun. Process of shrimp brain sauce[J]. Food Sci, 1991(7): 23-24. (in Chinese)
    [13]
    徐竞. 正交试验优化天然虾青素的提取工艺研究[J]. 南方水产, 2008, 4(2): 69-74. doi: 10.3969/j.issn.2095-0780.2008.02.011

    XU Jing. Study on the extraction of natural astaxanthin by orthogonal test[J]. South China Fish Sci. 2008, 4(2): 69-74. (in Chinese) doi: 10.3969/j.issn.2095-0780.2008.02.011
    [14]
    张华, 杨鑫, 马莺, 等. 固相萃取-反相高效液相色谱法同时测定饲料中的角黄素和虾青素[J]. 色谱, 2008, 26(3): 392-394. doi: 10.3321/j.issn:1000-8713.2008.03.027

    ZHANG Hua, YANG Xin, MA Ying, et al. Simultaneous determination of canthaxanthin and astaxanthin in feed stuffs using solid phase extraction-reversed-phase high performance liquid chromatography[J]. Chinese J Chromatogr, 2008, 26(3): 392-394. (in Chinese) doi: 10.3321/j.issn:1000-8713.2008.03.027
    [15]
    李福生, 徐宁, 李爱芬, 等. 一种稳定的雨生红球藻色素分析方法[J]. 食品科技, 2009, 34(7): 274-277.

    LI Fusheng, XU Ning, LI Aifen, et al. A stable method used for characterization of pigments in Haematococcus pluvialis[J]. Food Sci Technol, 2009, 34(7): 274-277. (in Chinese)
    [16]
    章超桦, 邓尚贵, 杨丽明, 等. 紫外线和温度对虾快速自溶的影响[J]. 湛江水产学院学报, 1994, 14(2): 51-56. https://xueshu.baidu.com/usercenter/paper/show?paperid=e43bc5e9e885b3563a36614ee8c10cb9&site=xueshu_se

    ZHANG Chaohua, DENG Shanggui, YANG Liming, et al. Effects of ultraviolet ray and temperature on quick autolysis of shrimp[J]. J Zhanjiang Fish College, 1994, 14(2): 51-56. (in Chinese) https://xueshu.baidu.com/usercenter/paper/show?paperid=e43bc5e9e885b3563a36614ee8c10cb9&site=xueshu_se
    [17]
    O'CONNOR I, O'BRIEN N. Modulation of UVA light-induced oxidative stress by beta-carotene, lutein and astaxanthin in culture fibroblasts[J]. Dermatol Sci, 1998, 16(3): 226-230. doi: 10.1016/s0923-1811(97)00058-3
    [18]
    李香春. 脂肪酶的研究进展[J]. 肉类工业, 2003(4): 45-48.

    LI Xiangchun. Progress in research on lipase[J]. Meat Ind, 2003(4): 45-48. (in Chinese)
    [19]
    赵仪, 陈兴才. 木瓜蛋白酶在虾仁加工废弃物中提取虾青素的应用[J]. 福州大学学报: 自然科学版, 2006, 34(3): 453-457. doi: 10.3969/j.issn.1000-2243.2006.03.031

    ZHAO Yi, CHEN Xingcai. The application of papain in astaxanthin extraction from prawn waste[J]. J Fuzhou Univ: Natural Science, 2006, 34(3): 453-457. (in Chinese) doi: 10.3969/j.issn.1000-2243.2006.03.031
    [20]
    钱飞, 刘海英, 过世东. 木瓜蛋白酶水解克氏原螯虾虾壳提取虾青素的研究[J]. 食品与生物技术学报, 2010, 29(2): 237-242.

    QIAN Fei, LIU Haiying, GUO Shidong. The application of papain in astaxanthin extraction from crayfish shell[J]. J Food Sci Biotechnol, 2010, 29(2): 237-242. (in Chinese)
    [21]
    张影霞, 武利刚, 罗志辉, 等. 虾青素的提取及其稳定性的研究[J]. 现代食品科技, 2008, 24(12): 1288-1291. doi: 10.3969/j.issn.1673-9078.2008.12.027

    ZHANG Yingxia, WU Ligang, LUO Zhihui, et al. Extraction of astaxanthin from shrimp and its stability[J]. Modern Food Sci Technol, 2008, 24(12): 1288-1291. (in Chinese) doi: 10.3969/j.issn.1673-9078.2008.12.027
  • Related Articles

    [1]YU Jie, ZOU Jianwei, CHEN Guobao. Characteristics of fish community structure and its relationship with environmental factors in marine ranching zone in southern area of Yintan in Guangxi[J]. South China Fisheries Science, 2024, 20(5): 81-90. DOI: 10.12131/20240102
    [2]LI Jiangtao, ZHANG Yanqiu, ZHANG Hong, LIU Chun, QIU Xiaolong, CHEN Ming, FANG Junchao, QIU Xiaotong, LIN Li, LYU Xiaojing. Effects of density stress on swimming behavior and muscle energy metabolism of Micropterus salmoides[J]. South China Fisheries Science, 2024, 20(2): 102-110. DOI: 10.12131/20230176
    [3]SHI Quan, GONG Yaping, SUN Feng, WANG Shuaijun, ZHANG Yanxin, WU Shu, ZHANG Di, CHEN Hui. Design and force calculation of a new type of composite anti-wind and wave aquaculture net cage in marine environment[J]. South China Fisheries Science, 2024, 20(1): 54-61. DOI: 10.12131/20230209
    [4]CAI Yancong, SUN Mingshuai, XU Youwei, CHEN Zuozhi. Spatial heterogeneity of relationship between distribution of Uroteuthis chinensis and marine environment in offshore waters of northern South China Sea[J]. South China Fisheries Science, 2023, 19(3): 1-10. DOI: 10.12131/20220288
    [5]SONG Wei, ZANG Na, FENG Yixuan, LIN Dongming. Study on lipid accumulation and contribution to reproductive energy in female Illex argentinus[J]. South China Fisheries Science, 2023, 19(1): 39-47. DOI: 10.12131/20210348
    [6]WU Peng, LIU Yong, XIAO Yayuan, XIE Yufang, TANG Guanglong, LIN Lin, WANG Teng, LI Chunhou. Evaluation of fisheries ecological environment in adjacent sea areas of Wanshan Archipelago in Pearl River Estuary in spring[J]. South China Fisheries Science, 2022, 18(5): 1-8. DOI: 10.12131/20210332
    [7]TANG Fenghua, CUI Xuesen, YANG Shenglong, ZHOU Weifeng, CHENG Tianfei, WU Zuli, ZHANG Heng. GIS analysis on effect of temporal and spatial patterns of marine environment on purse seine fishery in the western and central Pacific[J]. South China Fisheries Science, 2014, 10(2): 18-26. DOI: 10.3969/j.issn.2095-0780.2014.02.003
    [8]WANG Zenghuan, KE Changliang, WANG Xunuo, LI Liudong. Marine environmental quality assessment of shellfish culture in Liusha Bay[J]. South China Fisheries Science, 2011, 7(3): 24-30. DOI: 10.3969/j.issn.2095-0780.2011.03.005
    [9]LIN Lin, LI Chunhou, DU Feiyan, DAI Ming, HUANG Honghui. GIS-based comprehensive assessment of marine ecological environment quality in Daya Bay[J]. South China Fisheries Science, 2007, 3(5): 19-25.
    [10]LI Chunhou, QIN Honggui, JIA Xiaoping, TIAN Lixia. The effect of density on energy conversion efficiency of juvenile shrimp Litopenaeus vannamei[J]. South China Fisheries Science, 2006, 2(1): 30-33.
  • Cited by

    Periodical cited type(5)

    1. 陶欣,邱丽华,张博,王鹏飞,闫路路,赵超,林云想,林锋. 花鲈igf3基因SNP位点的鉴定及其与生长性状的关联分析. 基因组学与应用生物学. 2024(01): 109-116 .
    2. 杨琳,郭加民,王元浩,周思扬,李家乐,徐晓雁,沈玉帮. 青鱼生长QTL紧密连锁分子标记与生长性状的关联分析. 水生生物学报. 2024(08): 1358-1364 .
    3. 许艺兰,文露婷,黄姻,陈忠,覃俊奇,潘贤辉,周康奇,林勇,邓潜,武霞,杜雪松. 全州禾花鲤皮肤转录组特征分析. 河南农业科学. 2023(06): 150-159 .
    4. 余云登,朱浩拥,王盛南,王耀辉,马文超,杨晓,朱永祥,钱晓明,王秀利. 暗纹东方鲀leptin基因的克隆、SNP筛选及其与生长性状的关联分析. 大连海洋大学学报. 2021(02): 207-213 .
    5. 杨晓,马文超,杨金,苟盼盼,包玉龙,王秀利,刘圣聪,仇雪梅. 红鳍东方鲀B-FABP基因SNPs筛选及其与生长性状关联分析. 广东海洋大学学报. 2021(05): 28-34 .

    Other cited types(4)

Catalog

    Article views PDF downloads Cited by(9)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return