Volume 7 Issue 2
Apr.  2011
Turn off MathJax
Article Contents
Abstract
References
Related Articles
JIANG Miao, YANG Xianqing, LI Laihao, CEN Jianwei, HAO Shuxian, ZHANG Chaohua. Application of endogenous enzymes in astaxanthin extraction from shrimp shell[J]. South China Fisheries Science, 2011, 7(2): 55-60. DOI: 10.3969/j.issn.2095-0780.2011.02.009
Citation: JIANG Miao, YANG Xianqing, LI Laihao, CEN Jianwei, HAO Shuxian, ZHANG Chaohua. Application of endogenous enzymes in astaxanthin extraction from shrimp shell[J]. South China Fisheries Science, 2011, 7(2): 55-60. DOI: 10.3969/j.issn.2095-0780.2011.02.009
shu

Application of endogenous enzymes in astaxanthin extraction from shrimp shell

  • 1.

    College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524088, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

More Information
  • Received Date: September 15, 2010
  • Revised Date: November 25, 2010
    Graphical Abstract
    • Abstract

  • The extraction of astaxanthin from shrimp shell with endogenous enzyme is studied, and astaxanthin content in the extract is determined by high performance liquid chromatography (HPLC).The pH, temperature and hydrolysis time are considered as the factors to study the extraction effect. Orthogonal test is adopted for optimization, taking the yield of astaxanthin from shrimp shell as the evaluation indicator. The results show that the optimum process conditions are 4.0 pH, 50 ℃ and 1.5 h hydrolysis time, with the astaxanthin extraction from wet shrimp shell of 32.16 μg·g-1, which is 28% more than the extraction by direct ultrasonic treatment. No significant effect on the yield of astaxanthin is observed by adding lipase. Extracting astaxanthin by endogenous enzymes achieves better extraction effect and is economical.

    • FullText(HTML)
    • References (21)
  • [1]
    GOTO S, KOGURE K, ABE K, et al. Efficient radical trapping at the surface and inside the phospholipid membrane is responsible for highly potent antiperoxidative activity of the carotenoid astaxanthin[J]. Biochim Biophys Acta, 2001, 1512(2): 251-258. doi: 10.1016/S0005-2736(01)00326-1
    [2]
    NAGUIB Y M A. Antioxidant activities of astaxanthin and related carotenoids[J]. J Agric Food Chem, 2000, 48(4): 1150-1154. doi: 10.1021/jf991106k
    [3]
    SAVOUREN, BRIAND G, AMORYTMC, et al. Vitamin A status and metabolism of cutaneous polyamines in the hair-less mouse after UV irradiation: action of beta-carotene and astaxanthin[J]. Int J Vitam Nutr Res, 1995, 65(2): 79-86. https://pubmed.ncbi.nlm.nih.gov/7591536/
    [4]
    魏东, 严小君. 天然虾青素的超级抗氧化活性及其应用[J]. 中国海洋药物, 2001, 20(4): 45-50. doi: 10.3969/j.issn.1002-3461.2001.04.011

    WEI Dong, YAN Xiaojun. Super-antioxidant activity of natural astaxanthin and its application[J]. China J Mar Med, 2001, 20(4): 45-50. (in Chinese) doi: 10.3969/j.issn.1002-3461.2001.04.011
    [5]
    李艳艳, 翟占军, 张银花, 等. 虾青素在水产养殖上的应用[J]. 北京农学院学报, 2009, 24(2): 78-80. doi: 10.3969/j.issn.1002-3186.2009.02.021

    LI Yanyan, ZHAI Zhanjun, ZHANG Yinhua, et al. Application in aquatic animal of astaxanthin[J]. J Beijing Univ Agric, 2009, 24(2): 78-80. (in Chinese) doi: 10.3969/j.issn.1002-3186.2009.02.021
    [6]
    彭光华, 李忠, 张声华, 等. 6种类胡萝卜素对人乳腺癌细胞株细胞间隙连接通讯功能影响的比较[J]. 食品科学, 2008, 29(6): 45-48. doi: 10.3321/j.issn:1002-6630.2008.06.003

    PENG Guanghua, LI Zhong, ZHANG Shenghua, et al. Comparison of effect of six kinds of carotenoids on gap junctional intercellular communication of human breast cancer cell[J]. Food Sci, 2008, 29(6): 45-48. (in Chinese) doi: 10.3321/j.issn:1002-6630.2008.06.003
    [7]
    刘宏超, 杨丹. 从虾壳中提取虾青素工艺及其生物活性应用研究进展[J]. 化学试剂, 2009, 31(2), 105-108. doi: 10.3969/j.issn.0258-3283.2009.02.007

    LIU Hongchao, YANG Dan. Progress in research on extracting astaxanthin from shrimp shell and application of its biological activity[J]. Chem Reagent, 2009, 31(2), 105-108. (in Chinese) doi: 10.3969/j.issn.0258-3283.2009.02.007
    [8]
    焦雪峰. 虾青素在化妆品中的应用[J]. 广东化工, 2006, 33(1): 13-15. doi: 10.3969/j.issn.1007-1865.2006.01.007

    JIAO Xuefeng. Application of astaxanthin on cosmetics[J]. Guangdong Chem Ind, 2006, 33(1): 13-15. (in Chinese) doi: 10.3969/j.issn.1007-1865.2006.01.007
    [9]
    陆开形, 蒋霞敏, 翟兴文. 虾青素的生物学功能及应用[J]. 宁波大学学报, 2006, 16 (1): 95-98. doi: 10.3969/j.issn.1001-5132.2003.01.021

    LU Kaixing, JIANG Xiamin, ZHAI Xingwen. Biological function and application of astaxanthin[J]. J Ningbo Univ, 2006, 16(1): 95-98. (in Chinese) doi: 10.3969/j.issn.1001-5132.2003.01.021
    [10]
    胡雅馨, 惠伯棣, 李阳. 食用虾中虾青素类化合物的含量和组成[J]. 食品科学, 2007, 28, (8): 389-392. doi: 10.3321/j.issn:1002-6630.2007.08.094

    HU Yaxin, HUI Bodi, LI Yang. Content and composition of astaxanthin related compounds in shrimp[J]. Food Sci, 2007, 28(8): 389-392. (in Chinese) doi: 10.3321/j.issn:1002-6630.2007.08.094
    [11]
    何秋生, 李向阳, 方振华, 等. 淡水鱼的加工及综合利用初探[J]. 中国水产, 1999(7): 44-46.

    HE Qiusheng, LI Xiangyang, FANG Zhenhua, et al. The first exploration on processing and comprehensive unilization of freshwater fish[J]. China Fish, 1999(7): 44-46. (in Chinese)
    [12]
    顾晨光, 王建军. 虾脑酱的制作[J]. 食品科学, 1991(7): 23-24.

    GU Chenguang, WANG Jianjun. Process of shrimp brain sauce[J]. Food Sci, 1991(7): 23-24. (in Chinese)
    [13]
    徐竞. 正交试验优化天然虾青素的提取工艺研究[J]. 南方水产, 2008, 4(2): 69-74. doi: 10.3969/j.issn.2095-0780.2008.02.011

    XU Jing. Study on the extraction of natural astaxanthin by orthogonal test[J]. South China Fish Sci. 2008, 4(2): 69-74. (in Chinese) doi: 10.3969/j.issn.2095-0780.2008.02.011
    [14]
    张华, 杨鑫, 马莺, 等. 固相萃取-反相高效液相色谱法同时测定饲料中的角黄素和虾青素[J]. 色谱, 2008, 26(3): 392-394. doi: 10.3321/j.issn:1000-8713.2008.03.027

    ZHANG Hua, YANG Xin, MA Ying, et al. Simultaneous determination of canthaxanthin and astaxanthin in feed stuffs using solid phase extraction-reversed-phase high performance liquid chromatography[J]. Chinese J Chromatogr, 2008, 26(3): 392-394. (in Chinese) doi: 10.3321/j.issn:1000-8713.2008.03.027
    [15]
    李福生, 徐宁, 李爱芬, 等. 一种稳定的雨生红球藻色素分析方法[J]. 食品科技, 2009, 34(7): 274-277.

    LI Fusheng, XU Ning, LI Aifen, et al. A stable method used for characterization of pigments in Haematococcus pluvialis[J]. Food Sci Technol, 2009, 34(7): 274-277. (in Chinese)
    [16]
    章超桦, 邓尚贵, 杨丽明, 等. 紫外线和温度对虾快速自溶的影响[J]. 湛江水产学院学报, 1994, 14(2): 51-56. https://xueshu.baidu.com/usercenter/paper/show?paperid=e43bc5e9e885b3563a36614ee8c10cb9&site=xueshu_se

    ZHANG Chaohua, DENG Shanggui, YANG Liming, et al. Effects of ultraviolet ray and temperature on quick autolysis of shrimp[J]. J Zhanjiang Fish College, 1994, 14(2): 51-56. (in Chinese) https://xueshu.baidu.com/usercenter/paper/show?paperid=e43bc5e9e885b3563a36614ee8c10cb9&site=xueshu_se
    [17]
    O'CONNOR I, O'BRIEN N. Modulation of UVA light-induced oxidative stress by beta-carotene, lutein and astaxanthin in culture fibroblasts[J]. Dermatol Sci, 1998, 16(3): 226-230. doi: 10.1016/s0923-1811(97)00058-3
    [18]
    李香春. 脂肪酶的研究进展[J]. 肉类工业, 2003(4): 45-48.

    LI Xiangchun. Progress in research on lipase[J]. Meat Ind, 2003(4): 45-48. (in Chinese)
    [19]
    赵仪, 陈兴才. 木瓜蛋白酶在虾仁加工废弃物中提取虾青素的应用[J]. 福州大学学报: 自然科学版, 2006, 34(3): 453-457. doi: 10.3969/j.issn.1000-2243.2006.03.031

    ZHAO Yi, CHEN Xingcai. The application of papain in astaxanthin extraction from prawn waste[J]. J Fuzhou Univ: Natural Science, 2006, 34(3): 453-457. (in Chinese) doi: 10.3969/j.issn.1000-2243.2006.03.031
    [20]
    钱飞, 刘海英, 过世东. 木瓜蛋白酶水解克氏原螯虾虾壳提取虾青素的研究[J]. 食品与生物技术学报, 2010, 29(2): 237-242.

    QIAN Fei, LIU Haiying, GUO Shidong. The application of papain in astaxanthin extraction from crayfish shell[J]. J Food Sci Biotechnol, 2010, 29(2): 237-242. (in Chinese)
    [21]
    张影霞, 武利刚, 罗志辉, 等. 虾青素的提取及其稳定性的研究[J]. 现代食品科技, 2008, 24(12): 1288-1291. doi: 10.3969/j.issn.1673-9078.2008.12.027

    ZHANG Yingxia, WU Ligang, LUO Zhihui, et al. Extraction of astaxanthin from shrimp and its stability[J]. Modern Food Sci Technol, 2008, 24(12): 1288-1291. (in Chinese) doi: 10.3969/j.issn.1673-9078.2008.12.027
    • Related Articles

  • Related Articles

    [1]LIU Yuhang, XIANG Huan, HUANG Hui, WEI Ya, CEN Jianwei, PAN Chuang, LI Chunsheng, ZHAO Yongqiang, HAO Shuxian. Optimization of process and mechanism of extracting astaxanthin from shrimp shells with deep eutectic solvent[J]. South China Fisheries Science, 2024, 20(3): 164-172. DOI: 10.12131/20230225
    [2]WANG Jiamei, TANG Zhenzhao, DING Ling, JIA Xiaoping, ZHANG Zhongzhe. Study on local scour of artificial reef based on flume test[J]. South China Fisheries Science, 2020, 16(6): 32-38. DOI: 10.12131/20200074
    [3]GU Liangbin, XU Liwen, FENG Juan, SU YouLu, LIU Guangfeng, GUO Zhixun. Identification and drug sensitive test of bacterial Pathogens from Plectropomus leopardus with tail fester disease[J]. South China Fisheries Science, 2015, 11(4): 71-80. DOI: 10.3969/j.issn.2095-0780.2015.04.011
    [4]LIU Jian, HUANG Hongliang, WU Yue, RAO Xin, LI Lingzhi, CHEN Shuai, YANG Jialiang, ZHOU Bin. Model test of hydrodynamic characteristics of two types of vertical cambered slotted otter boards[J]. South China Fisheries Science, 2015, 11(1): 68-74. DOI: 10.3969/j.issn.2095-0780.2015.01.010
    [5]YE Le, ZHAO Wang, WU Kaichang. Acute toxicity tests of three antiparasitic drugs to juvenile snail of Babylonia areolata[J]. South China Fisheries Science, 2014, 10(4): 22-26. DOI: 10.3969/j.issn.2095-0780.2014.04.004
    [6]DING Aixia, HE Yi′er. Test on release and proliferation of Pseudosciaena crocea in Daiquyang sea area[J]. South China Fisheries Science, 2011, 7(1): 73-77. DOI: 10.3969/j.issn.2095-0780.2011.01.012
    [7]YANG Xueming, ZHANG Li, LI Youning, CHEN Mingqiang, GAN Xi. Primary test on artificial spawning induction and larvae rearing of Holothuria scabra[J]. South China Fisheries Science, 2011, 7(1): 39-44. DOI: 10.3969/j.issn.2095-0780.2011.01.006
    [8]HU Zehui, XU Junzhuo, WANG Yuebin, CHAI Xuejun. Optimization of SRAP-PCR system for Portunus trituberculatus based on orthogonal experimental design[J]. South China Fisheries Science, 2010, 6(5): 68-73. DOI: 10.3969/j.issn.1673-2227.2010.05.011
    [9]YANG Qibin, LI Xiaolan, SUN Miaomiao, WEN Weigeng, HUANG Jianhua. Methods of testing vitality of postlarvae of Penaeus monodon[J]. South China Fisheries Science, 2010, 6(1): 12-18. DOI: 10.3969/j.issn.1673-2227.2010.01.003
    [10]XU Jing. Study on the extraction of natural astaxanthin by orthogonal test[J]. South China Fisheries Science, 2008, 4(2): 69-74.

Catalog

    Get Citation
    PDF
    XML
    Article views (4561) PDF downloads (2570) Cited by()
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return