SHEN Yongfu, GENG Liang, GONG Yi, DING Yu, LI Yunkai, DENG Jiangshan, CHEN Haonan. Effects of preservation methodology on multi-tissue stable isotope signatures of oceanic squid: a case study of jumbo squid (Dosidicus gigas)[J]. South China Fisheries Science, 2023, 19(5): 162-167. DOI: 10.12131/20230091
Citation: SHEN Yongfu, GENG Liang, GONG Yi, DING Yu, LI Yunkai, DENG Jiangshan, CHEN Haonan. Effects of preservation methodology on multi-tissue stable isotope signatures of oceanic squid: a case study of jumbo squid (Dosidicus gigas)[J]. South China Fisheries Science, 2023, 19(5): 162-167. DOI: 10.12131/20230091

Effects of preservation methodology on multi-tissue stable isotope signatures of oceanic squid: a case study of jumbo squid (Dosidicus gigas)

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  • Received Date: April 27, 2023
  • Revised Date: May 23, 2023
  • Accepted Date: June 04, 2023
  • Available Online: August 14, 2023
  • Carbon and nitrogen stable isotope technology (δ13C and δ15N) is an important tool for the study of trophic ecology of marine organisms. However, there is no unified standard for the preservation methods in cephalopods' samples, which may cause biases in stable isotope values and easily lead to misleading conclusions. Thus, we assessed the effects of preservation method (Freezing, 75% ethanol solution and 38% formaldehyde solution) and duration (0, 30 and 180 d) on the stable isotope ratios of carbon and nitrogen of tissues (Muscle, beak and gonad) of jumbo squid (Dosidicus gigas). The results show that freezing affected δ13C and C/N values of muscle and gonad within 30 d (P<0.05), with offsets of (−0.89±0.30)‰ and 0.22±0.15, respectively, which were lower than the effects of alcohol and formaldehyde. After 30 days of storage in the alcohol solution, significant changes were observed for muscle [δ13C: (−0.24±0.42)‰ , δ15N: (0.17±1.07)‰ and C/N: (0.32±0.49)] and gonad [δ13C: (−0.36±0.44), C/N: (0.21±0.14)] (P<0.05), but no significant change was found in beak (P>0.05). Formaldehyde preservation induced a decrease in δ13C [(−3.03±1.87)‰] and δ15N [(−0.48±0.72)‰] but an increase in C/N (0.86±0.73) within 30 d (P<0.05). However, the δ15N and C/N values significantly increased in beak and gonad stored in formaldehyde on the 180th day (P<0.05). Therefore, it should be careful to use the preserved soft tissue samples during stable isotope analysis, while hard tissue, such as horny jaw, can be preserved by freezing or ethanol solution.

  • [1]
    ROUNICK J S, WINTERBOURN M J. Stable carbon isotopes and carbon flow in ecosystems[J]. Bioscience, 1986, 36: 171-177. doi: 10.2307/1310304
    [2]
    HOBSON K A, PIATT J F, PITOCCHELLI J. Using stable isotopes to determine seabird trophic relationships[J]. J Anim Ecol, 1994, 63(4): 786-798. doi: 10.2307/5256
    [3]
    PETERSON B J, FRY B. Stable isotopes in ecosystem studies[J]. Ann Rev Ecol System, 1987, 18: 293-320. doi: 10.1146/annurev.es.18.110187.001453
    [4]
    WAN Y, HU J Y, AN L H, et al. Determination of trophic relationships within a Bohai Bay food web using stable δ15N and δ13C analysis[J]. Chin Sci Bull, 2005, 50(10): 1021-1025. doi: 10.1360/04wd0283
    [5]
    HUSSEY N E, MACNEIL M A, OLIN J A, et al. Stable isotopes and elasmobranchs: tissue types, methods, applications and assumptions[J]. J Fish Biol, 2012, 80(5): 1449-1484. doi: 10.1111/j.1095-8649.2012.03251.x
    [6]
    GAO X D, GONG Y, CHEN X J, et al. Dietary shifts and niche partitioning throughout ontogeny avoid intraspecific competition in a pelagic generalist predator[J]. Mar Ecol Prog Ser, 2022, 692: 81-97. doi: 10.3354/meps14079
    [7]
    GONG Y, LI Y K, CHEN X J, et al. Trophic niche and diversity of a pelagic squid (Dosidicus gigas): a comparative study using stable isotope, fatty acid, and feeding apparatuses morphology[J]. Front Mar Sci, 2020, 7: 00642. doi: 10.3389/fmars.2020.00642
    [8]
    汪惠琼, 陈洁南, 李云凯, 等. 厄尔尼诺对柔鱼亚科近缘种茎柔鱼与鸢乌贼营养生态位的影响[J]. 海洋渔业, 2020, 42(5): 524-532.
    [9]
    高小迪, 贡艺, 陈新军, 等. 东太平洋赤道海域茎柔鱼的营养生态位和肠道微生物组成[J]. 应用生态学报, 2021, 32(3): 1087-1095.
    [10]
    李云凯, 汪惠琼, 陈新军, 等. 柔鱼科近缘种茎柔鱼与鸢乌贼营养生态位及相互关系[J]. 生态学报, 2020, 40(15): 5418-5423.
    [11]
    QUSIPE-MACHACA M, GUZMAN-RIVAS F A, IBANEZ C M, et al. Trophodynamics of the jumbo squid Dosidicus gigas during winter in the Southeast Pacific Ocean off the coast of Chile: diet analyses and fatty acid profile[J]. Fishs Res, 2022, 245: 106154. doi: 10.1016/j.fishres.2021.106154
    [12]
    EVANS K, HINDELL M A. The diet of sperm whales (Physeter macrocephalus) in southern Australian waters[J]. ICES J Mar Sci, 2004, 61(8): 1313-1329. doi: 10.1016/j.icesjms.2004.07.026
    [13]
    SARAKINOS H C, JOHNSON M L, VANDER ZANDEN M J. A synthesis of tissue preservation effects on carbon and nitrogen stable isotope signatures[J]. Can J Zool, 2002, 80(02): 381-387. doi: 10.1139/z02-007
    [14]
    FEUCHTMAYR H, GREY J. Effect of preparation and preservation procedures on carbon and nitrogen stable isotope determinations from zooplankton[J]. Rapid Commun Mass Spectrom, 2003, 126(17): 2605-2610.
    [15]
    李月, 刘晃, 谢正丽. 样本预处理方式对罗氏沼虾碳和氮稳定同位素比值检测的影响[J]. 核农学报, 2022, 36(10): 1975-1983.
    [16]
    HETHERINGTON E D, KURLE C M, OHMAN M D, et al. Effects of chemical preservation on bulk and amino acid isotope ratios of zooplankton, fish, and squid tissues[J]. Rapid Commun Mass Spectrom, 2019, 33(10): 935-945. doi: 10.1002/rcm.8408
    [17]
    KAEHLER S, PAKHOMOV E A. Effects of storage and preservation on the delta C-13 and delta N-15 signatures of selected marine organisms[J]. Mar Ecol Prog Ser, 2001, 219: 299-304. doi: 10.3354/meps219299
    [18]
    CARABEL S, VERISIMO P, FREIRE J. Effects of preservatives on stable isotope analyses of four marine species[J]. Est Coast Shelf Sci, 2009, 82(2): 348-350. doi: 10.1016/j.ecss.2009.01.011
    [19]
    von ENDT D W. Spirit collections: a preliminary analysis of some organic materials found in the storage fluids of mammals[J]. Collection Forum, 1994, 10: 10-19.
    [20]
    BOURG B L, LEPOINT G, MICHEL L N. Effects of preservation methodology on stable isotope compositions of sea stars[J]. Rapid Commun Mass Spectrom, 2020, 34(2): e8589.
    [21]
    POST D M, LAYMAN C A, ARRINGTON D A, et al. Getting to the fat of the matter: models, methods and assumptions for dealing with lipids in stable isotope analyses[J]. Oceologica, 2007, 152: 179-189. doi: 10.1007/s00442-006-0630-x
    [22]
    林静远, 刘必林, 金宵. 茎柔鱼角质颚的机械强度特性[J]. 上海海洋大学学报, 2020, 29(3): 385-391.
    [23]
    SHEN Y F, DAVID M, GONG Y, et al. Effects of ethanol storage and lipid extraction on stable isotope compositions of twelve pelagic predators[J]. Front Mar Sci, 2023, 10: 1118013. doi: 10.3389/fmars.2023.1118013
    [24]
    HORII S, TAKAHASHI K, FURUYA K. Effects of ethanol-preservation on stable carbon and nitrogen isotopic signatures in marine predators[J]. Plankton Benthos Res, 2015, 10(2): 91-97. doi: 10.3800/pbr.10.91
    [25]
    MICHAEL D R, TED O, DAVID E. Effects of formalin preservation on invertebrate stable isotope values over decadal time scales[J]. Can J Zool, 2012, 90(11): 1320-1327. doi: 10.1139/z2012-101
    [26]
    UMBRICHT J, DIPPNER J W, FRY B, et al. Correction of the isotopic composition (δ13C and δ15N) of preserved Baltic and North Sea macrozoobenthos and their trophic interactions[J]. Mar Ecol Prog Ser, 2018, 595: 1-13. doi: 10.3354/meps12543
    [27]
    BICKNELL A W J, CAMPBELL M, KNIGHT M E, et al. Effects of formalin preservation on stable carbon and nitrogen isotope signatures in Calanoid copepods: implications for the use of Continuous Plankton Recorder Survey samples in stable isotope analyses[J]. Rapid Commun Mass Spectrom, 2011, 25(13): 1794-1800. doi: 10.1002/rcm.5049
    [28]
    BENNETT-WILLIAMS J, SKINNER C, WYATT A S J, et al. A multi-tissue, multi-species assessment of lipid and urea stable isotope biases in mesopredator elasmobranchs[J]. Front Mar Sci, 2022, 9: 821478. doi: 10.3389/fmars.2022.821478
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