Volume 19 Issue 5
Oct.  2023
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SHEN Yongfu, GENG Liang, GONG Yi, DING Yu, LI Yunkai, DENG Jiangshan, CHEN Haonan. Effects of preservation methodology on multi-tissue stable isotope signatures of oceanic squid: a case study of jumbo squid (Dosidicus gigas)[J]. South China Fisheries Science, 2023, 19(5): 162-167. DOI: 10.12131/20230091
Citation: SHEN Yongfu, GENG Liang, GONG Yi, DING Yu, LI Yunkai, DENG Jiangshan, CHEN Haonan. Effects of preservation methodology on multi-tissue stable isotope signatures of oceanic squid: a case study of jumbo squid (Dosidicus gigas)[J]. South China Fisheries Science, 2023, 19(5): 162-167. DOI: 10.12131/20230091
shu

Effects of preservation methodology on multi-tissue stable isotope signatures of oceanic squid: a case study of jumbo squid (Dosidicus gigas)

  • 1.

    College of Marine Sciences, Shanghai Ocean University, Shanghai 201306, China

  • 2.

    Key Laboratory of Sustainable Exploitation of Oceanic Fisheries Resources, Ministry of Education, Shanghai 201306, China

  • 3.

    National Engineering Research Center for Oceanic Fisheries, Shanghai 201306, China

  • 4.

    Key Laboratory of Oceanic Fisheries Exploration, Ministry of Agriculture and Rural Affairs, Shanghai 201306, China

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  • Received Date: April 27, 2023
  • Revised Date: May 23, 2023
  • Accepted Date: June 04, 2023
  • Available Online: August 14, 2023
    Graphical Abstract
    • Abstract

  • Carbon and nitrogen stable isotope technology (δ13C and δ15N) is an important tool for the study of trophic ecology of marine organisms. However, there is no unified standard for the preservation methods in cephalopods' samples, which may cause biases in stable isotope values and easily lead to misleading conclusions. Thus, we assessed the effects of preservation method (Freezing, 75% ethanol solution and 38% formaldehyde solution) and duration (0, 30 and 180 d) on the stable isotope ratios of carbon and nitrogen of tissues (Muscle, beak and gonad) of jumbo squid (Dosidicus gigas). The results show that freezing affected δ13C and C/N values of muscle and gonad within 30 d (P<0.05), with offsets of (−0.89±0.30)‰ and 0.22±0.15, respectively, which were lower than the effects of alcohol and formaldehyde. After 30 days of storage in the alcohol solution, significant changes were observed for muscle [δ13C: (−0.24±0.42)‰ , δ15N: (0.17±1.07)‰ and C/N: (0.32±0.49)] and gonad [δ13C: (−0.36±0.44), C/N: (0.21±0.14)] (P<0.05), but no significant change was found in beak (P>0.05). Formaldehyde preservation induced a decrease in δ13C [(−3.03±1.87)‰] and δ15N [(−0.48±0.72)‰] but an increase in C/N (0.86±0.73) within 30 d (P<0.05). However, the δ15N and C/N values significantly increased in beak and gonad stored in formaldehyde on the 180th day (P<0.05). Therefore, it should be careful to use the preserved soft tissue samples during stable isotope analysis, while hard tissue, such as horny jaw, can be preserved by freezing or ethanol solution.

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