Morphological characterization of embryonic development in <i>Rana catesbeiana</i>

CHEN Qiuyu; ZHENG Xiaoting; ZHONG Jinsong; CHEN Zhibing; WANG Ying; LIANG Xueying; DONG Hongbiao; ZHANG Jiasong

doi:10.12131/20230015

Volume 19 Issue 6

Dec. 2023

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South China Fisheries Science > 2023 > 19(6): 158-165. > DOI: 10.12131/20230015

CHEN Qiuyu, ZHENG Xiaoting, ZHONG Jinsong, CHEN Zhibing, WANG Ying, LIANG Xueying, DONG Hongbiao, ZHANG Jiasong. Morphological characterization of embryonic development in Rana catesbeiana[J]. South China Fisheries Science, 2023, 19(6): 158-165. DOI: 10.12131/20230015

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Morphological characterization of embryonic development in Rana catesbeiana

1.
College of Fisheries and Life Science, Dalian Ocean University, Dalian 116023, China
2.
South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs, Guangzhou 510300, China
3.
Zhongyang Fishery (Qingyuan) Co. Ltd., Qingyuan 513129, China
4.
Agro-Tech Extension Center of Guangdong Province, Guangzhou 510145, China

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Received Date: February 09, 2023
Revised Date: June 13, 2023
Accepted Date: August 03, 2023
Available Online: August 03, 2023

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Abstract

Abstract

To explore the early embryonic development process of bullfrogs (Rana catesbeiana) and supplement their biological information and imaging data, we obtained the fertilized eggs from artificially bred bullfrogs by natural oviposition to investigate their morphological changes and biological characteristics. The results show that the size of fertilized eggs was (1.41±0.31) mm. At temperature of (24.5±0.4) ℃, the early embryonic development process could be divided into 7 phases and 24 stages. It took 177 h 38 min for the fertilized eggs to reach the operculum closure stage, and the total accumulated temperature was 4 249.71 h·℃. The embryonic development process showed a pattern of a fast speed at the first several stages (From fertilized egg to 64-cell stage) and then a slowdown at the latter stages. When being stimulated by external stimuli, the embryo had an annular contraction response by bending the head and tail. Later, as the embryo hatched until the completion of gill closure, they become tadpoles.
- Rana catesbeiana,
- Embryonic development,
- Morphological characteristics,
- Fertilized eggs

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