Volume 16 Issue 6
Dec.  2020
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WANG Yang, ZHAO Jing, WANG Jingru, LUO Yunlong, LIU Ying, BAI Dongqing, SHAO Peng, LI Yuhan. Inhibitory activity of home-made garbage enzyme against aquatic pathogens and isolation and identification of its fermentation strains[J]. South China Fisheries Science, 2020, 16(6): 97-104. DOI: 10.12131/20200101
Citation: WANG Yang, ZHAO Jing, WANG Jingru, LUO Yunlong, LIU Ying, BAI Dongqing, SHAO Peng, LI Yuhan. Inhibitory activity of home-made garbage enzyme against aquatic pathogens and isolation and identification of its fermentation strains[J]. South China Fisheries Science, 2020, 16(6): 97-104. DOI: 10.12131/20200101
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Inhibitory activity of home-made garbage enzyme against aquatic pathogens and isolation and identification of its fermentation strains

  • College of Fisheries, Tianjin Agricultural University/Tianjin Key Laboratory of Aqua-ecology and Aquaculture, Tianjin 300384, China

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  • Received Date: May 10, 2020
  • Revised Date: July 13, 2020
  • Accepted Date: August 17, 2020
  • Available Online: September 27, 2020
    Graphical Abstract
    • Abstract

  • In order to evaluate the inhibitory activity of home-made garbage enzyme against aquatic pathogens and to isolate their fermentation strains, we determined the minimum inhibitory concentrations of the home-made garbage enzyme on the aquatic pathogenic microorganisms Vibrio candida SP-1, V. harveyi SP-1, V. rotiferianus SP-1 and Aeromonas hydrophila 245 as 11.5%, 11%, 6.2% and 11% in volume fraction by turbidimetric method. The minimum bactericidal concentrations were determined as 16%, 16%, 16% and 12% in volume fraction by flat line scribing method. The challenge test proves that the supernatant of home-made garbage enzyme decreased (90.5±6.49) % of the mortality in zebrafish induced by A. hydrophila. The sensitivity tests of pH, temperature and protease prove that the main antibacterial active substances of home-made garbage enzyme were acidic substances that were stable to heating and protease treatment. Furthermore, the primary fermentation strain was separated from the garbage enzyme and identified as Pichia kudriavzevii by ITS sequence analysis. The results provide a theoretical basis for applying home-made garbage enzyme in combating bacterial diseases in aquaculture.

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