Volume 17 Issue 2
Apr.  2021
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HE Yang, DAI Xuping, FAN Wei, WANG Jun, QIN Chuanjie, CHEN Jie, CAO Xinyu. Location of blood-spleen barrier of Pelteobagrus vachelli by Trypan blue staining[J]. South China Fisheries Science, 2021, 17(2): 44-50. DOI: 10.12131/20200005
Citation: HE Yang, DAI Xuping, FAN Wei, WANG Jun, QIN Chuanjie, CHEN Jie, CAO Xinyu. Location of blood-spleen barrier of Pelteobagrus vachelli by Trypan blue staining[J]. South China Fisheries Science, 2021, 17(2): 44-50. DOI: 10.12131/20200005
shu

Location of blood-spleen barrier of Pelteobagrus vachelli by Trypan blue staining

  • 1.

    Neijiang Normal University/Key Laboratory of Sichuan Province for Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River, Neijiang 641000, China

  • 2.

    College of Life Science, Neijiang Normal University, Neijiang 641000, China

  • 3.

    Neijiang Academy of Agricultural Sciences, Neijiang 641000, China

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  • Received Date: January 10, 2020
  • Revised Date: December 02, 2020
  • Available Online: January 03, 2021
    Graphical Abstract
    • Abstract
  • To explore the structure of the spleen of Pelteobagruvs vachelli and the location of its blood-spleen barrier, we observed its spleen in morphology, size, histology and in vivo staining. The results show that the spleen of P. vachelli was located in the middle of the abdominal cavity with dark red colour, and the spleen body index was 0.120%–0.273%. Paraffin sections of healthy spleen were prepared and stained with HE and modified James, and the microscopic observation reveals that the spleen tissue was mainly composed of underdeveloped capsule system, unclear boundary between red and white pulp, and fibers that were composed of reticular fibers and collagen fibers. Reticular fibers were mainly located around the splenic cord and ellipsoid; collagen fibers were mainly located in the splenic cord. We applied Trypan blue in vivo staining method to explore the position and function of the blood-spleen barrier, and found that Trypan blue was mainly located in the ellipsoid endothelial cells of the spleen, and first appeared at 4th hour after injection. It accumulated in the ellipsoid gradually along with time, and the number increased significantly after 24 h, and continued to increase until 72nd hour. It is shown that the ellipsoid endothelial cells are an important component of the blood-spleen barrier.
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