WANG Jian, ZENG Benhe, XU Zhaoli, ZHANG Bianbian, LIU Haiping, WANG Wanliang, WANG Jinlin, ZHOU Jianshe, HUANG Liping. Effect of dietary protein level on digestive enzyme activity and histological structure in intestine and liver of juvenile Schizopygopsis younghusbandi[J]. South China Fisheries Science, 2019, 15(6): 112-119. DOI: 10.12131/20190107
Citation: WANG Jian, ZENG Benhe, XU Zhaoli, ZHANG Bianbian, LIU Haiping, WANG Wanliang, WANG Jinlin, ZHOU Jianshe, HUANG Liping. Effect of dietary protein level on digestive enzyme activity and histological structure in intestine and liver of juvenile Schizopygopsis younghusbandi[J]. South China Fisheries Science, 2019, 15(6): 112-119. DOI: 10.12131/20190107

Effect of dietary protein level on digestive enzyme activity and histological structure in intestine and liver of juvenile Schizopygopsis younghusbandi

More Information
  • Received Date: May 18, 2019
  • Revised Date: June 20, 2019
  • Accepted Date: July 07, 2019
  • Available Online: July 16, 2019
  • To explore the effects of different feed protein levels on the digestive enzyme activity and histological structure in the intestine and liver of juvenile Schizopygopsis younghusbandi, we designed six kinds of equal-energy experimental feeds with different protein levels (20%, 25%, 30%, 35%, 40%, 45%) to feed the juveniles with initial body mass of (22.42±0.56) g for 60 d. The results show that with the increase of feed protein level, the protease activity in intestine and liver increased first then declined. The protease activity in 35% protein group was significantly higher than that in the other groups (P<0.05). The lipase and amylase activities reduced significantly (P<0.05). The wall thickness and villus height of foregut, midgut and hindgut all increased and then decreased (P<0.05). The wall thickness of foregut and midgut were highest in 30% group, while the wall thickness of the hindgut was highest in the 35% group. The villus height of foregut, midgut and hindgut were highest in 30% group. Feed protein level higher than 35% may damage liver tissue structure. Under this condition, considering the digestive enzymes activity and histological structure of intestines and liver, the optimum protein level of juvenile S. younghusbandi should be 30%.

  • [1]
    陈义方, 李卓佳, 牛津, 等. 饲料蛋白水平对不同规格凡纳滨对虾蛋白质表观消化率和消化酶活性的影响[J]. 南方水产科学, 2012, 08(5): 66-71. doi: 10.3969/j.issn.2095-0780.2012.05.010
    [2]
    孙翰昌, 徐敬明, 庞敏. 饲料蛋白水平对瓦氏黄颡鱼消化酶活性的影响[J]. 水生态学杂志, 2010, 31(2): 84-88.
    [3]
    吴永恒, 王秋月, 冯政夫, 等. 饲料粗蛋白含量对刺参消化酶及消化道结构的影响[J]. 海洋科学, 2012, 36(1): 36-41. doi: 10.3969/j.issn.1671-6647.2012.01.005
    [4]
    LEIGH S C, NGUYEN-PHUC B Q, GERMAN D P. The effects of protein and fiber content on gut structure and function in zebrafish (Danio rerio)[J]. J Comp Physiol B, 2018, 188: 237-253. doi: 10.1007/s00360-017-1122-5
    [5]
    段友健. 拉萨裸裂尻鱼个体生物学和种群动态研究[D]. 武汉: 华中农业大学, 2015: 93-94.
    [6]
    李芳. 西藏尼洋河流域水生生物研究及水电工程对其影响的预测评价[D]. 西安: 西北大学, 2006: 69-73.
    [7]
    王孝平, 邢树礼. 考马斯亮法测定蛋白质含量的研[J]. 天津化工, 2009, 23(3): 40-41. doi: 10.3969/j.issn.1008-1267.2009.03.016
    [8]
    王常安, 户国, 孙鹏, 等. 饲料蛋白质和脂肪水平对亚东鲑亲鱼生长性能、消化酶活性和血清指标的影响[J]. 动物营养学报, 2017, 29(2): 571-582. doi: 10.3969/j.issn.1006-267x.2017.02.025
    [9]
    李成, 秦溱, 李金龙, 等. 不同蛋白水平饲料对光倒刺鲃幼鱼生长, 消化酶及体成分的影响[J]. 饲料工业, 2018, 39(24): 34-39.
    [10]
    梁萍, 秦志清, 林建斌, 等. 饲料中不同蛋白质水平对半刺厚唇鱼幼鱼生长性能及消化酶活性的影响[J]. 中国农学通报, 2018, 34(2): 136-140. doi: 10.11924/j.issn.1000-6850.casb17090056
    [11]
    桑永明, 杨瑶, 尹航, 等. 饲料蛋白水平对方正银鲫幼鱼生长, 体成分, 肝脏生化指标和肠道消化酶活性的影响[J]. 水生生物学报, 2018, 42(4): 736-743. doi: 10.7541/2018.090
    [12]
    RAMESH R, DUBE K, REDDY A K, et al. Effect of varying protein levels on growth and digestive enzyme activities of pengba Osteobrama belangeri (Valenciennes, 1844)[J]. Ind J Fish, 2017, 64: 206-213.
    [13]
    MENDEZ-MARTINEZ Y, GARCIA-GUERRERO M U, MARTINEZ-CORDOVA L R, et al. Effect of different ratios of dietary protein-energy on growth, body proximal composition, digestive enzyme activity, and hepatopancreas histology in Macrobrachium americanum (Bate, 1868) prawn juveniles[J]. Aquaculture, 2018, 485: 1-11. doi: 10.1016/j.aquaculture.2017.11.012
    [14]
    TOK N C, JAIN K K, PRABU D L, et al. Metabolic and digestive enzyme activity of Pangasianodon hypophthalmus (Sauvage, 1878) fingerlings in response to alternate feeding of different protein levels in the diet[J]. Aquacult Res, 2017, 48(6): 2895-2911. doi: 10.1111/are.2017.48.issue-6
    [15]
    SILVA W S, COSTA L S, LÓPEZ-OLMEDA J F, et al. Gene expression, enzyme activity and performance of Nile tilapia larvae fed with diets of different CP levels[J]. Animal, 2019, 13(7): 1376-1384. doi: 10.1017/S175173111800318X
    [16]
    米海峰. 不同蛋白源和大豆抗营养因子对牙鲆蛋白消化酶的活性与基因表达的影响[D]. 青岛: 中国海洋大学, 2008: 39-43.
    [17]
    LÓPEZ-LÓPEZ S, NOLASCO H, VILLARREAL-COLMENARES H A. Digestive enzyme response to supplemental ingredients in practical diets for juvenile freshwater crayfish Cherax quadricarinatus[J]. Aquacult Nut, 2005, 11(2): 79-85.
    [18]
    吴本丽, 黄龙, 何吉祥, 等. 长期饥饿后异育银鲫对饲料蛋白质的需求[J]. 动物营养学报, 2018, 30(6): 2215-2225. doi: 10.3969/j.issn.1006-267x.2018.06.025
    [19]
    赵书燕, 林黑着, 黄忠, 等. 不同蛋白质水平下添加小肽对石斑鱼生长、消化酶、血清生化和抗氧化能力的影响[J]. 南方水产科学, 2016, 12(3): 15-23. doi: 10.3969/j.issn.2095-0780.2016.03.003
    [20]
    ZHANG W, LIU K, TAN B P, et al. Transcriptome, enzyme activity and histopathology analysis reveal the effects of dietary carbohydrate on glycometabolism in juvenile largemouth bass, Micropterus salmoides[J]. Aquaculture, 2019, 504: 39-51. doi: 10.1016/j.aquaculture.2019.01.030
    [21]
    WANG L G, HU S Y, LOU B, et al. Effect of different dietary protein and lipid levels on the growth, body composition, and intestinal digestive enzyme activities of juvenile yellow drum Nibea albiflora (Richardson)[J]. J Ocean Univ Chin, 2018, 17(5): 1261-1267. doi: 10.1007/s11802-018-3660-1
    [22]
    HEIKKINEN J, VIELMA J, KEMILAINEN O, et al. Effects of soybean meal based diet on growth performance, gut histopathology and intestinal microbiota of juvenile rainbow trout (Oncorhynchus mykiss)[J]. Aquaculture, 2006, 261(1): 259-268. doi: 10.1016/j.aquaculture.2006.07.012
    [23]
    CHEN F J, WANG H C. Study on histological structure of intestine in Gymnocypris przewalskii with different age[J]. P Vet Med, 2013, 34(1): 34-37.
    [24]
    WEI Y L, LIANG M Q, ZHENG K K, et al. The effects of fish protein hydrolysate on the digestibility of juvenile turbot (Scophthalmus maximus L)[J]. Acta Hydrobiol Sinica, 2014, 38(5): 910-920.
    [25]
    徐静. 蛋白对生长中期草鱼生产性能、肠道、机体和鳃健康及肌肉品质的作用及其作用机制[D]. 雅安: 四川农业大学, 2016: 34-35.
    [26]
    孙金辉, 范泽, 张美静, 等. 饲料蛋白水平对鲤幼鱼肝功能和抗氧化能力的影响[J]. 南方水产科学, 2017, 13(3): 113-119. doi: 10.3969/j.issn.2095-0780.2017.03.015
    [27]
    李坚明, 甘晖, 冯广朋, 等. 饲料脂肪含量与奥尼罗非鱼幼鱼肝脏形态结构特征的相关性[J]. 南方水产, 2008, 4(5): 37-43. doi: 10.3969/j.issn.2095-0780.2008.05.006
  • Related Articles

    [1]ZHU Shuli, CHEN Weitao, WU Zhi, XIA Yuguo, YANG Jiping, LI Yuefei, LI Jie. Preliminary investigation of fish diversity in middle and lower reaches of Pearl River based on environmental DNA technology[J]. South China Fisheries Science, 2024, 20(1): 120-129. DOI: 10.12131/20230111
    [2]XIE Yufang, WU Peng, LIU Yong, XIAO Yayuan, TANG Guanglong, WANG Teng, LIN Lin, LI Chunhou. Study on habitat suitability of Coilia mystus in Pearl River Estuary, China[J]. South China Fisheries Science, 2023, 19(1): 22-29. DOI: 10.12131/20220029
    [3]LI Hongting, ZHANG Shuai, ZOU Keshu, CHEN Zuozhi, CHEN Xiaolei, JIANG Peiwen, CAO Yiting, LI Min. Establishment and optimization of environmental DNA extraction method from water of Pearl River Estuary[J]. South China Fisheries Science, 2022, 18(3): 30-37. DOI: 10.12131/20210304
    [4]CHEN Zhi, CAI Xingwei, ZHANG Qingfeng, LI Gaojun, MA Chunlai, SHEN Zhixin. Preliminary construction and comparative analysis of environmental DNA metabarcoding reference database of freshwater fishes in Hainan Island[J]. South China Fisheries Science, 2022, 18(3): 1-12. DOI: 10.12131/20210339
    [5]XIONG Pengli, CHEN Zuozhi, HOU Gang, ZHANG Shuai, QIU Yongsong, FAN Jiangtao, XU Shannan. Decadal change in biological traits of Collichthys lucidus in Pearl River Estuary[J]. South China Fisheries Science, 2021, 17(6): 31-38. DOI: 10.12131/20210072
    [6]YAN Yali, ZHANG Nan, GUO Huayang, GUO Liang, ZHU Kecheng, LIU Baosuo, ZHANG Dianchang. Species identification and phylogenetic relationship in Siganidae based on DNA barcoding[J]. South China Fisheries Science, 2019, 15(1): 100-105. DOI: 10.12131/20180083
    [7]SHUAI Fangmin, LI Zhiquan, LIU Guowen, LI Xinhui, LI Yuefei, YANG Jiping, LI Jie. Resource status of Japanese eel (Anguilla japonica) in the Pearl River Estuary[J]. South China Fisheries Science, 2015, 11(2): 85-89. DOI: 10.3969/j.issn.2095-0780.2015.02.012
    [8]GAO Yuan, LAI Zini, WANG Chao, PANG Shixun, WEI Taili, XIE Wenping, YANG Wanling. Community characteristics of zooplankton in Pearl River Estuary in summer of 2006[J]. South China Fisheries Science, 2008, 4(1): 10-15.
    [9]WANG Di, LIN Zhaojin. Spatial and temporal variations of fish community structure in the Pearl River Estuary waters[J]. South China Fisheries Science, 2006, 2(4): 37-45.
    [10]YANG Lin, ZHANG Xufeng, ZHANG Peng, TAN Yongguang. Composition of by-catch of shrimping beam trawl in the Pearl River Estuary, China[J]. South China Fisheries Science, 2005, 1(1): 27-34.
  • Cited by

    Periodical cited type(11)

    1. 郭瑶杰,万武波,王海山,叶乐,陈治. 3组常用鱼类eDNA宏条形码通用引物对三亚水环境样品的物种检出效果比较. 南方水产科学. 2025(01): 66-76 . 本站查看
    2. 薛向平,孙朝徽,刘霞,惠筠,李学军,李秀启,司飞. 基于COI基因的黄河河南-山东段鲤群体遗传结构分析. 淡水渔业. 2025(02): 43-52 .
    3. 朱书礼,陈蔚涛,武智,夏雨果,杨计平,李跃飞,李捷. 基于环境DNA技术的珠江中下游鱼类多样性初步研究. 南方水产科学. 2024(01): 120-129 . 本站查看
    4. 于耀鲜,邢冉冉,邓婷婷,王琳,卢思远,王宏勋,王丽梅,陈颖. 基于重组酶介导等温扩增-CRISPR/Cas12a的青鱼物种快速鉴定研究. 食品安全质量检测学报. 2024(04): 40-48 .
    5. 徐颖琪,梁绪虹,陈新军,宋成辉,彭祖焜,王丛丛. 基于COⅠ基因构建西北太平洋常见鱼类DNA条形码参考数据库. 上海海洋大学学报. 2024(04): 823-835 .
    6. 张浩博,王晓艳,陈治,钟兰萍,高天翔. 基于环境DNA metabarcoding的舟山及其邻近海域鱼类空间分布格局的初步研究. 水产学报. 2024(08): 125-138 .
    7. 李筱芹,吴开阳,倪达富,杨丽亚,鲁桃秀,张连博,邓华堂,吴彤飞,何荣超,付梅,姚维志,吕红健. 基于环境DNA技术的梯级水坝对长江上游重要支流鱼类多样性的影响研究——以綦江为例. 生态学报. 2024(19): 8865-8883 .
    8. 陈炳耀,信誉,路方婷,孙婧,刘杉,李敏,吴斌,王崇瑞,张衡,周玉香,白玲. 长江江豚监测现状及展望. 中国环境监测. 2023(02): 1-10 .
    9. 陈治,蔡杏伟,申志新,张清凤,李芳远,谷圆,李高俊,赵光军,王镇江. 海南岛淡水鱼类eDNA宏条形码COⅠ通用引物的筛选. 渔业科学进展. 2023(06): 40-57 .
    10. 刘乙蒙,刘洋,刘必林,俞晔伟,宋成辉,王丛丛. Cyt b和12S rRNA基因条形码在灯笼鱼科鱼类物种鉴定中的应用. 中国水产科学. 2023(09): 1112-1126 .
    11. 蒋佩文,李敏,张帅,陈作志,徐姗楠. 基于环境DNA宏条码和底拖网的珠江河口鱼类多样性. 水生生物学报. 2022(11): 1701-1711 .

    Other cited types(5)

Catalog

    Article views (5012) PDF downloads (38) Cited by(16)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return