Volume 16 Issue 1
Feb.  2020
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WEI Wenjuan, ZHAO Shu, WANG Yuan, ZHOU Junfang, LI Xincang, FANG Wenhong. Detection of antibiotics resistance and distribution of resistance genes in Vibrio parahaemolyticus from cultured shrimp[J]. South China Fisheries Science, 2020, 16(1): 9-16. DOI: 10.12131/20190165
Citation: WEI Wenjuan, ZHAO Shu, WANG Yuan, ZHOU Junfang, LI Xincang, FANG Wenhong. Detection of antibiotics resistance and distribution of resistance genes in Vibrio parahaemolyticus from cultured shrimp[J]. South China Fisheries Science, 2020, 16(1): 9-16. DOI: 10.12131/20190165
shu

Detection of antibiotics resistance and distribution of resistance genes in Vibrio parahaemolyticus from cultured shrimp

  • 1.

    Shanghai Ocean University/National Demonstration Center for Experimental Fisheries Science Education/National Pathogen Collection Center for Aquatic Animals, Shanghai 201306, China

  • 2.

    East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of East China Sea Fishery Resources Development and Utilization, Ministry of Agriculture and Rural Affairs, Shanghai 200090, China

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  • Received Date: August 21, 2019
  • Revised Date: November 21, 2019
  • Available Online: December 02, 2019
    Graphical Abstract
    • Abstract
  • We used agar dilution method to detect the resistance of 36 strains of Vibrio parahaemolyticus to 16 drugs. And used PCR amplication and DNA sequencing to detect the antimicrobial resistance to quinolones (qnrA, qnrB, qnrS, qnrVC), phenicols (cat, optrA, floR, cfr), tetracyclines (tetA, tetB, tetM), sulfonamides (sul1, sul2, sul3), aminoglycosides (strA, strB, aadA, aacA), rifampicin (arr), β-lactams (carB) and macrolides (erm). The results indicate that the isolates exhibited high resistance to ampicillin (88.9%) and sulfamethoxazole (SMZ, 66.7%), all susceptible to neomycin sulfate, gentamicin, ceftriaxone and meropenem. In general, multi-drug resistance (MDR) was highly prevalent (61.1%), and one isolate was resistant to six antimicrobials. Furthermore, 72.2% and 58.3% of the isolates were primarily mediated by qnrVC and strB, respectively; and the macrolides and rifamycin resistant genes were not detected in all the isolates. Obvious mismatch was found between the antimicrobial resistance phenotypes and genotypes, revealing the complexity of resistance to V. parahaemolyticus.
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