JIANG Kui, XU Liwen, SU Youlu, WANG Yu, GUO Zhixun, XU Haidong, GAO Fang, FENG Juan. Analysis of antibiotic resistance spectrum of Vibrio harveyi strains isolated from maricultured fish in the South China Sea during 2012~2014[J]. South China Fisheries Science, 2016, 12(6): 99-107. DOI: 10.3969/j.issn.2095-0780.2016.06.013
Citation: JIANG Kui, XU Liwen, SU Youlu, WANG Yu, GUO Zhixun, XU Haidong, GAO Fang, FENG Juan. Analysis of antibiotic resistance spectrum of Vibrio harveyi strains isolated from maricultured fish in the South China Sea during 2012~2014[J]. South China Fisheries Science, 2016, 12(6): 99-107. DOI: 10.3969/j.issn.2095-0780.2016.06.013

Analysis of antibiotic resistance spectrum of Vibrio harveyi strains isolated from maricultured fish in the South China Sea during 2012~2014

More Information
  • Received Date: December 10, 2015
  • Revised Date: February 22, 2016
  • Vibrio harveyi, a main pathogen of maricultured fish, causes great economic loss in aquaculture industry. We tested the temporal and spatial distribution of drug resistance of 90 V. harveyi strains isolated from maricultured fish in the South China Sea by Kirby-bauer diffusion method. Phylogenetic of drug resistance was analyzed by squared Euclidean distance using bacteriostatic rings diameter of 12 kinds of antibiotics. The diversity of the drug resistance spectrum of 90 strains was also discussed. Results show that 90 V. harveyi strains formed 26 antibiogram types and antibiogram abundance was 28.9%. Thirty-five strains, occuping 38.9% of total strains, had 21 multi-drug antibiogram types (resistant to ≥ 3 antibiotics), which was 80.8% of spectral richness. The antibiogram types show significant difference of 90 V. harveyi strains in years and regions: 10, 18 and 8 in 2012, 2013 and 2014 years, respectively, while 17, 13 and 2 kinds in Hainan, Guangdong and Guangxi Provinces correspondently. Phylogenetic and cluster analysis indicate that 90 strains separated to Group Ⅰ, Group Ⅱ and others clusters. Eleven antibiogram types with abundance of 16.9% in Group Ⅰ, and 12 antibiogram types with 57.1% in Group Ⅱ, while four kinds with 100% in other clusters. Group Ⅰ and Group Ⅱ could be divided into five subgroups of ⅰ~ⅴ. The results indicate that V. harveyi strains display complex antibiogram types in Hainan, Guangdong and Guangxi Provinces, and the resistance spectrum of V. harveyi changes and expands as time goes on.

  • [1]
    BAUMANN P, FURNISS A, LEE J. Genus 1. Vibrio pacini 1854, 411AL[M]//KRIEG N R, HOLT J G. Bergey' s manual of systematic bacteriology, Volume 1. USA: Williams & Wilkins, 1984: 518-538.
    [2]
    杨少丽, 王印庚, 董树刚. 海水养殖鱼类弧菌病的研究进展[J]. 海洋水产研究, 2005, 26(4): 75-83. doi: 10.3969/j.issn.1000-7075.2005.04.013
    [3]
    HASHEM M, EL-BARBARY M. Vibrio harveyi infection in Arabian Surgeon fish (Acanthurus sohal) of Red Sea at Hurghada, Egypt[J]. Egy J Aquat Res, 2013, 39(3): 199-203. doi: 10.1016/j.ejar.2013.10.006
    [4]
    SOTO-RODRIGUEZ S A, GOMEZ-GIL B, LOZANO R A, et al. Virulence of Vibrio harveyi responsible for the "Bright-red" Syndrome in the Pacific white shrimp Litopenaeus vannamei[J]. J Invertebr Pathol, 2012, 109(3): 307-317. doi: 10.1016/j.jip.2012.01.006
    [5]
    郝贵杰, 沈锦玉, 潘晓义, 等. 抗哈维氏弧菌脂多糖单克隆抗体的制备及其鉴定[J]. 上海水产大学学报, 2008, 17(3): 274-279. http://shhydxxb.ijournals.cn/shhy/article/abstract/20080361?st=article_issue
    [6]
    宋超. 我国常见病原菌的耐药现状及其进展[J]. 中国现代药物应用, 2008, 2(24): 185-186. doi: 10.3969/j.issn.1673-9523.2008.24.153
    [7]
    SARTER S, NGUYEN H N K, HUNG T L, et al. Antibiotic resistance in Gram-negative bacteria isolated from farmed catfish[J]. Food Control, 2007, 18(11): 1391-1396. doi: 10.1016/j.foodcont.2006.10.003
    [8]
    张致平. 抗耐药菌药物研究进展[J]. 中国抗生素杂志, 2005, 30(7): 430-440. doi: 10.3969/j.issn.1001-8689.2005.07.015
    [9]
    JAYARAMAN R. Antibiotic resistance: an overview of mechanisms and a paradigm shift[J]. Curr Sci, 2009, 96(11): 1475-1484. https://www.researchgate.net/publication/242102256_Antibiotic_resistance_An_overview_of_mechanisms_and_a_paradigm_shift
    [10]
    TENDENCIA E A, de la PEÑA L D. Antibiotic resistance of bacteria from shrimp ponds[J]. Aquaculture, 2001, 195(3/4): 193-204. https://www.sciencedirect.com/science/article/abs/pii/S0044848600005706
    [11]
    TEO W P, TAN M C, POH C L. Genetic determinants of tetracycline resistance in Vibrio harveyi[J]. Antimicrob Agents Chemother, 2002, 46(4): 1038-1045. doi: 10.1128/AAC.46.4.1038-1045.2002
    [12]
    王瑞旋, 耿玉静, 冯娟, 等. 杂色鲍哈维弧菌耐药质粒的鉴定和分析[J]. 南方水产科学, 2012, 8(2): 1-6. doi: 10.3969/j.issn.2095-0780.2012.02.001
    [13]
    MOLINA-AJA A, GARCÍA-GASCA A, ABREU-GROBOIS A, et al. Plasmid profiling and antibiotic resistance of Vibrio strains isolated from cultured penaeid shrimp[J]. FEMS Microbiol Lett, 2002, 213(1): 7-12. doi: 10.1111/j.1574-6968.2002.tb11278.x
    [14]
    PARVATHI A, MENDEZ D, ANTO C. Distribution of putative virulence genes and antimicrobial drug resistance in Vibrio harveyi[J]. Ind J Microbiol, 2011, 51(3): 332-337. doi: 10.1007/s12088-011-0128-z
    [15]
    翁幸鐾, 糜祖煌, 金辉. 多重耐药大肠埃希菌获得性耐药基因检测及指标聚类分析[J]. 中华临床感染病杂志, 2011, 4(3): 154-158, 181. doi: 10.3760/cma.j.issn.1674-2397.2011.03.007
    [16]
    许云敏, 晋臻, 杜艳, 等. 878株鲍曼不动杆菌的耐药分析及流行调查[J]. 昆明医学院学报, 2011, 32(9): 92-95. https://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_kmyxyxb201109026
    [17]
    王春艳, 阎斌伦. 连云港市赣榆县水产养殖环境中哈氏弧菌耐药性研究[J]. 淮海工学院学报(自然科学版), 2013, 22(1): 88-91. doi: 10.3969/j.issn.1672-6685.2013.01.023
    [18]
    席劲瑛, 黄晶晶, 胡洪营, 等. 污水处理厂二级出水中四环素抗性菌的生长特性与耐药性[J]. 环境科学学报, 2014, 34(7): 1724-1729. doi: 10.13671/j.hjkxxb.2014.0505
    [19]
    RAISSY M, MOUMENI M, ANASARI M, et al. Antibiotic resistance pattern of some Vibrio strains isolated from seafood[J]. Iran J Fish Sci, 2012, 11(3): 618-626. https://www.researchgate.net/publication/277181069_Antibiotic_resistance_pattern_of_some_Vibrio_strains_isolated_from_seafood
    [20]
    MUSA N, WEI L S, WEE W. Phenotypic and genotypic characteristics of Vibrio harveyi isolated from black tiger shimp (Penaeus monodon)[J]. World Appl Sci J, 2008, 3(6): 885-902.
    [21]
    中华人民共和国卫生部. 纸片法抗菌药物敏感试验标准(WS/T 125—1999)[S]. 北京: 中国标准出版社, 2000: 13-14.
    [22]
    CLSI. Performance standards for antimicrobial disk susceptibility tests; approved standard-eleventh edition. CLSL document M02-A11[S]. Wayne, PA: CLSI, 2012: 8-52.
    [23]
    CLSI. Performance standards for antimicrobial disk susceptibility tests; twenty-second informational supplement. CLSL document M100-S22[S]. Wayne, PA: CLSI, 2012: 20-161.
    [24]
    CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard ninth-editon. CLSL document M07-A9[S]. Wayne, PA: CLSI, 2012: 16-42.
    [25]
    李景云, 马越, 孙自镛, 等. 聚类分析在大肠埃希菌耐药性研究中的应用[J]. 中华微生物学和免疫学杂志, 2003, 23(5): 384-387. doi: 10.3760/j:issn:0254-5101.2003.05.023
    [26]
    CHELOSSI E, VEZZULLI L, MILANO A, et al. Antibiotic resistance of benthic bacteria in fish-farm and control sediments of the Western Mediterranean[J]. Aquaculture, 2003, 219(1/2/3/4): 83-97. https://www.sciencedirect.com/science/article/abs/pii/S0044848603000164
    [27]
    KUMAR P A, JOSEPH B, PATTERSON J. Antibiotic and heavy metal resistance profile of pathogens isolated from infected fish in Tuticorin, south-east coast of India[J]. Ind J Fish, 2011, 58(2): 121-125.
    [28]
    王瑞旋, 耿玉静, 王江勇, 等. 水产致病菌耐药基因的研究[J]. 海洋环境科学, 2012, 31(3): 323-328. doi: 10.3969/j.issn.1007-6336.2012.03.004
    [29]
    ZHANG R F, WANG Y L, GU J D. Identification of environmental plasmid-bearing Vibrio species isolated from polluted and pristine marine reserves of Hong Kong, and resistance to antibiotics and mercury[J]. Antonie Van Leeuwenhoek, 2006, 89(3/4): 307-315. doi: 10.1007/s10482-005-9032-z
    [30]
    曾德乾, 冯娟, 徐力文, 等. 海水养殖鱼哈维弧菌分离株的耐药谱型分析[J]. 中国水产科学, 2015, 22(1): 129-138. https://www.zhangqiaokeyan.com/academic-journal-cn_journal-fishery-sciences-china_thesis/0201261545633.html
    [31]
    王瑞旋, 王江勇, 徐力文, 等. 军曹鱼养殖水体及其肠道弧菌的耐药性研究[J]. 南方水产, 2007, 5(5): 1-6. https://www.schinafish.cn/article/id/e1418cc0-8a77-4a8c-b31a-6b692abb67e6
    [32]
    耿红, 宣莹莹, 蔡夏童, 等. 太原市2014年春节期间常规大气污染物浓度变化及聚类分析[J]. 环境科学学报, 2015, 35(4): 965-974. doi: 10.13671/j.hjkxxb.2014.0957
    [33]
    张晶, 胡学钢, 张斌. 基于蜕变关系的聚类程序测试方法[J]. 电子测量与仪器学报, 2011, 25(8): 688-694. doi: 10.3724/SP.J.1187.2011.00688
    [34]
    满其蒙, 冯娟, 区又君, 等. 15株鱼源致病性鱼诺卡氏菌的聚类分析[J]. 南方水产科学, 2013, 5(5): 86-92. doi: 10.3969/j.issn.2095-0780.2013.05.014?pageType=en
  • Related Articles

    [1]WU Xinyan, LIANG Hongwei, LUO Xiangzhong, SHA Hang, ZOU Guiwei. Effects of morphological traits on body mass of Changfeng silver carp (Hypophthalmichthys molitrix) at different ages[J]. South China Fisheries Science, 2021, 17(3): 62-69. DOI: 10.12131/20200265
    [2]FANG Wei, ZHOU Shengjie, ZHAO Wang, YANG Rui, HU Jing, YU Gang, MA Zhenhua. Correlation and path analysis of morphological traits to body mass of juvenile Thunnus albacores[J]. South China Fisheries Science, 2021, 17(1): 52-58. DOI: 10.12131/20200158
    [3]CHEN Jian, GUO Dan, ZHAI Ziqing, YU Dahui, BAI Lirong. Correlation analysis of morphological traits and body mass traits of Coelomactra antiquata[J]. South China Fisheries Science, 2021, 17(1): 45-51. DOI: 10.12131/20200180
    [4]LI Fenghui, LIU Kun, WANG Pengfei, BIAN Li, CHEN Siqing, CHANG Qing, GE Jianlong, ZHOU Liqing. Morphological structure and karyotype of Thamnaconus septentrionalis[J]. South China Fisheries Science, 2019, 15(3): 104-112. DOI: 10.12131/20180118
    [5]HAN Qingpeng, LU Huajie, CHEN Xinjun, JIN Yue, CHEN Guobao. Morphological analysis of beaks of Uroteuthis chinensis in the northern South China Sea[J]. South China Fisheries Science, 2017, 13(4): 122-130. DOI: 10.3969/j.issn.2095-0780.2017.04.015
    [6]LIU Yongxin, LIU Yi, LIU Yingjie, YANG Runqing. Allometric analysis of body weight and morphological traits for Japanese flounder(Paralichthys olivaceus)[J]. South China Fisheries Science, 2016, 12(1): 36-42. DOI: 10.3969/j.issn.2095-0780.2016.01.006
    [7]TAN Caigang, LIU Baosuo, ZHANG Dongling, NIU Zhikai, ZHANG Bo, CHEN Mingqiang, FAN Sigang, JIANG Song, HUANG Guiju, LI Youning, CHEN Suwen, YU Dahui. Analysis of grey relationship between morphological traits and body weight of pearl oyster (Pinctada fucata)[J]. South China Fisheries Science, 2015, 11(2): 35-40. DOI: 10.3969/j.issn.2095-0780.2015.02.005
    [8]WANG Xu, XU Heng, ZOU Li, DU Xiao, WANG Fengli, ZHENG Wei, HAN Zhiqiang, SHUI Bonian. Morphological variation analysis of three differente geographic populations of Monodonta labio[J]. South China Fisheries Science, 2013, 9(4): 22-27. DOI: 10.3969/j.issn.2095-0780.2013.04.004
    [9]WANG Yin, LIU Shuji, SU Yongchang, HUANG Yu, WU Chengye. Morphological analysis and nutrition evaluation of Paphia undulate[J]. South China Fisheries Science, 2011, 7(6): 19-25. DOI: 10.3969/j.issn.2095-0780.2011.06.004
    [10]ZHANG Wei, LI Chunhou, JIA Xiaoping, CHEN Peimao, FANG Liang. Multivariate analysis of organisms communities attached on concrete and iron reefs[J]. South China Fisheries Science, 2009, 5(3): 30-35. DOI: 10.3969/j.issn.1673-2227.2009.03.005
  • Cited by

    Periodical cited type(10)

    1. 孙阿君,丁炜东,曹丽萍,曹哲明,邴旭文. 氨氮胁迫对翘嘴鳜幼鱼抗氧化酶、消化酶活性及应激相关基因表达的影响. 水产科技情报. 2024(01): 44-51 .
    2. 王筱,冼健安,张秀霞,张泽龙,李军涛,郑佩华,吴恒梅,鲁耀鹏. 方斑东风螺人工养殖、环境生理和营养需求研究进展. 中国饲料. 2024(03): 83-88+117 .
    3. 张钰伟,赵旺,邓正华,黄星美,温为庚,孙敬锋,王瑞旋. 芽孢杆菌对方斑东风螺生长、存活、免疫及消化酶活性的影响. 南方水产科学. 2023(01): 106-115 . 本站查看
    4. 梁晶,邢诒炫,吕布,臧战,刘子岭,唐贤明,於锋,Hebert Ely Vasquez,战欣,郑兴,顾志峰. 底砂粒径对方斑东风螺生长、消化、抗氧化及底质的影响. 水产科技情报. 2023(03): 137-145 .
    5. 梅泊承,张晓东,赵淳朴,徐继林,王丹丽,郭春阳. 串联养殖模式下的凡纳滨对虾(Litopenaeus vannamei)与缢蛏(Sinonovacula constricta)生长特性、消化免疫及水生态效应. 海洋与湖沼. 2023(03): 907-920 .
    6. 赵旺,温为庚,谭春明,黄星美,杨蕊,陈明强,杨其彬,陈旭. 饥饿胁迫对猛虾蛄溶菌酶和消化酶活性的影响. 水产科技情报. 2022(01): 30-35 .
    7. 谭春明,赵旺,马振华,于刚. 红腹海参消化道指标、组织学和酶活性的季节变化. 南方水产科学. 2022(05): 39-45 . 本站查看
    8. 周建聪,顾志峰,叶丙聪,刘闯,羊玉梅,刘春胜,王爱民,石耀华. 盐度和氨氮对方斑东风螺存活和能量收支的影响. 海洋科学. 2022(10): 104-112 .
    9. 韩朝婕,陈屹洋,贺振楠,张严匀,周文礼,高金伟,贾旭颖. 氨氮胁迫对水产动物生长、消化酶及免疫影响的研究进展. 河北渔业. 2021(05): 32-35 .
    10. 丁炜东,曹丽萍,曹哲明,邴旭文. 氨氮胁迫对翘嘴鳜幼鱼鳃、消化道酶活力的影响. 南方水产科学. 2020(03): 31-37 . 本站查看

    Other cited types(6)

Catalog

    Recommendations
    Embryonic and larval development of sea urchin (temnopleurus reevesii)
    YU Weihuan et al., SOUTH CHINA FISHERIES SCIENCE, 2025
    Effects of acute hypoxia stress on respiratory metabolism and related gene expression of juvenilebabylonia areolate
    REN Xiaoyao et al., SOUTH CHINA FISHERIES SCIENCE, 2025
    Early pigmented cell development and body color change ofplatax teira
    YU Chuxia et al., SOUTH CHINA FISHERIES SCIENCE, 2025
    Analysis of changes in intestinal structure and microbial composition inelentheronema tetradactylumjuvenile at different days of age
    FENG Yuantai et al., SOUTH CHINA FISHERIES SCIENCE, 2025
    Comparative analysis of growth and muscle nutritional components of hybrid snakehead "male snakehead no.1" under different breeding modes#br##br#
    YU Hong et al., FISHERY MODERNIZATION, 2025
    Morphological differences analysis of hybrid (chinemys reevesii ♀ x ocadia sinensis ♂) and their parents #br#
    He Gang et al., CHINESE JOURNAL OF FISHERIES, 2023
    Comparative genomics analysis reveals genetic characteristics and nitrogen fixation of
    Zhong, Chaofang et al., ISCIENCE, 2024
    Metagenomics reveals the temporal dynamics of the rumen resistome and microbiome in goat kids
    Chai, Jianmin et al., MICROBIOME, 2024
    Comparison of small biopsy and cytology specimens: subtyping of pulmonary adenocarcinoma
    CYTOJOURNAL, 2023
    Characterization of temporomandibular joint articular disc progenitor cell clones
    EUROPEAN CELLS & MATERIALS, 2023
    Powered by
    Article views (3389) PDF downloads (981) Cited by(16)
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return