Volume 12 Issue 6
Dec.  2016
Turn off MathJax
Article Contents
Abstract
References
Related Articles
JIANG Kui, XU Liwen, SU Youlu, WANG Yu, GUO Zhixun, XU Haidong, GAO Fang, FENG Juan. Analysis of antibiotic resistance spectrum of Vibrio harveyi strains isolated from maricultured fish in the South China Sea during 2012~2014[J]. South China Fisheries Science, 2016, 12(6): 99-107. DOI: 10.3969/j.issn.2095-0780.2016.06.013
Citation: JIANG Kui, XU Liwen, SU Youlu, WANG Yu, GUO Zhixun, XU Haidong, GAO Fang, FENG Juan. Analysis of antibiotic resistance spectrum of Vibrio harveyi strains isolated from maricultured fish in the South China Sea during 2012~2014[J]. South China Fisheries Science, 2016, 12(6): 99-107. DOI: 10.3969/j.issn.2095-0780.2016.06.013
shu

Analysis of antibiotic resistance spectrum of Vibrio harveyi strains isolated from maricultured fish in the South China Sea during 2012~2014

  • 1.

    Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 210306, China

  • 3.

    Tropical Aquaculture Research and Development Center of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Sanya 572018, China

More Information
  • Received Date: December 10, 2015
  • Revised Date: February 22, 2016
    Graphical Abstract
    • Abstract

  • Vibrio harveyi, a main pathogen of maricultured fish, causes great economic loss in aquaculture industry. We tested the temporal and spatial distribution of drug resistance of 90 V. harveyi strains isolated from maricultured fish in the South China Sea by Kirby-bauer diffusion method. Phylogenetic of drug resistance was analyzed by squared Euclidean distance using bacteriostatic rings diameter of 12 kinds of antibiotics. The diversity of the drug resistance spectrum of 90 strains was also discussed. Results show that 90 V. harveyi strains formed 26 antibiogram types and antibiogram abundance was 28.9%. Thirty-five strains, occuping 38.9% of total strains, had 21 multi-drug antibiogram types (resistant to ≥ 3 antibiotics), which was 80.8% of spectral richness. The antibiogram types show significant difference of 90 V. harveyi strains in years and regions: 10, 18 and 8 in 2012, 2013 and 2014 years, respectively, while 17, 13 and 2 kinds in Hainan, Guangdong and Guangxi Provinces correspondently. Phylogenetic and cluster analysis indicate that 90 strains separated to Group Ⅰ, Group Ⅱ and others clusters. Eleven antibiogram types with abundance of 16.9% in Group Ⅰ, and 12 antibiogram types with 57.1% in Group Ⅱ, while four kinds with 100% in other clusters. Group Ⅰ and Group Ⅱ could be divided into five subgroups of ⅰ~ⅴ. The results indicate that V. harveyi strains display complex antibiogram types in Hainan, Guangdong and Guangxi Provinces, and the resistance spectrum of V. harveyi changes and expands as time goes on.

    • FullText(HTML)
    • References (34)
  • [1]
    BAUMANN P, FURNISS A, LEE J. Genus 1. Vibrio pacini 1854, 411AL[M]//KRIEG N R, HOLT J G. Bergey' s manual of systematic bacteriology, Volume 1. USA: Williams & Wilkins, 1984: 518-538.
    [2]
    杨少丽, 王印庚, 董树刚. 海水养殖鱼类弧菌病的研究进展[J]. 海洋水产研究, 2005, 26(4): 75-83. doi: 10.3969/j.issn.1000-7075.2005.04.013
    [3]
    HASHEM M, EL-BARBARY M. Vibrio harveyi infection in Arabian Surgeon fish (Acanthurus sohal) of Red Sea at Hurghada, Egypt[J]. Egy J Aquat Res, 2013, 39(3): 199-203. doi: 10.1016/j.ejar.2013.10.006
    [4]
    SOTO-RODRIGUEZ S A, GOMEZ-GIL B, LOZANO R A, et al. Virulence of Vibrio harveyi responsible for the "Bright-red" Syndrome in the Pacific white shrimp Litopenaeus vannamei[J]. J Invertebr Pathol, 2012, 109(3): 307-317. doi: 10.1016/j.jip.2012.01.006
    [5]
    郝贵杰, 沈锦玉, 潘晓义, 等. 抗哈维氏弧菌脂多糖单克隆抗体的制备及其鉴定[J]. 上海水产大学学报, 2008, 17(3): 274-279. http://shhydxxb.ijournals.cn/shhy/article/abstract/20080361?st=article_issue
    [6]
    宋超. 我国常见病原菌的耐药现状及其进展[J]. 中国现代药物应用, 2008, 2(24): 185-186. doi: 10.3969/j.issn.1673-9523.2008.24.153
    [7]
    SARTER S, NGUYEN H N K, HUNG T L, et al. Antibiotic resistance in Gram-negative bacteria isolated from farmed catfish[J]. Food Control, 2007, 18(11): 1391-1396. doi: 10.1016/j.foodcont.2006.10.003
    [8]
    张致平. 抗耐药菌药物研究进展[J]. 中国抗生素杂志, 2005, 30(7): 430-440. doi: 10.3969/j.issn.1001-8689.2005.07.015
    [9]
    JAYARAMAN R. Antibiotic resistance: an overview of mechanisms and a paradigm shift[J]. Curr Sci, 2009, 96(11): 1475-1484. https://www.researchgate.net/publication/242102256_Antibiotic_resistance_An_overview_of_mechanisms_and_a_paradigm_shift
    [10]
    TENDENCIA E A, de la PEÑA L D. Antibiotic resistance of bacteria from shrimp ponds[J]. Aquaculture, 2001, 195(3/4): 193-204. https://www.sciencedirect.com/science/article/abs/pii/S0044848600005706
    [11]
    TEO W P, TAN M C, POH C L. Genetic determinants of tetracycline resistance in Vibrio harveyi[J]. Antimicrob Agents Chemother, 2002, 46(4): 1038-1045. doi: 10.1128/AAC.46.4.1038-1045.2002
    [12]
    王瑞旋, 耿玉静, 冯娟, 等. 杂色鲍哈维弧菌耐药质粒的鉴定和分析[J]. 南方水产科学, 2012, 8(2): 1-6. doi: 10.3969/j.issn.2095-0780.2012.02.001
    [13]
    MOLINA-AJA A, GARCÍA-GASCA A, ABREU-GROBOIS A, et al. Plasmid profiling and antibiotic resistance of Vibrio strains isolated from cultured penaeid shrimp[J]. FEMS Microbiol Lett, 2002, 213(1): 7-12. doi: 10.1111/j.1574-6968.2002.tb11278.x
    [14]
    PARVATHI A, MENDEZ D, ANTO C. Distribution of putative virulence genes and antimicrobial drug resistance in Vibrio harveyi[J]. Ind J Microbiol, 2011, 51(3): 332-337. doi: 10.1007/s12088-011-0128-z
    [15]
    翁幸鐾, 糜祖煌, 金辉. 多重耐药大肠埃希菌获得性耐药基因检测及指标聚类分析[J]. 中华临床感染病杂志, 2011, 4(3): 154-158, 181. doi: 10.3760/cma.j.issn.1674-2397.2011.03.007
    [16]
    许云敏, 晋臻, 杜艳, 等. 878株鲍曼不动杆菌的耐药分析及流行调查[J]. 昆明医学院学报, 2011, 32(9): 92-95. https://med.wanfangdata.com.cn/Paper/Detail/PeriodicalPaper_kmyxyxb201109026
    [17]
    王春艳, 阎斌伦. 连云港市赣榆县水产养殖环境中哈氏弧菌耐药性研究[J]. 淮海工学院学报(自然科学版), 2013, 22(1): 88-91. doi: 10.3969/j.issn.1672-6685.2013.01.023
    [18]
    席劲瑛, 黄晶晶, 胡洪营, 等. 污水处理厂二级出水中四环素抗性菌的生长特性与耐药性[J]. 环境科学学报, 2014, 34(7): 1724-1729. doi: 10.13671/j.hjkxxb.2014.0505
    [19]
    RAISSY M, MOUMENI M, ANASARI M, et al. Antibiotic resistance pattern of some Vibrio strains isolated from seafood[J]. Iran J Fish Sci, 2012, 11(3): 618-626. https://www.researchgate.net/publication/277181069_Antibiotic_resistance_pattern_of_some_Vibrio_strains_isolated_from_seafood
    [20]
    MUSA N, WEI L S, WEE W. Phenotypic and genotypic characteristics of Vibrio harveyi isolated from black tiger shimp (Penaeus monodon)[J]. World Appl Sci J, 2008, 3(6): 885-902.
    [21]
    中华人民共和国卫生部. 纸片法抗菌药物敏感试验标准(WS/T 125—1999)[S]. 北京: 中国标准出版社, 2000: 13-14.
    [22]
    CLSI. Performance standards for antimicrobial disk susceptibility tests; approved standard-eleventh edition. CLSL document M02-A11[S]. Wayne, PA: CLSI, 2012: 8-52.
    [23]
    CLSI. Performance standards for antimicrobial disk susceptibility tests; twenty-second informational supplement. CLSL document M100-S22[S]. Wayne, PA: CLSI, 2012: 20-161.
    [24]
    CLSI. Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically; approved standard ninth-editon. CLSL document M07-A9[S]. Wayne, PA: CLSI, 2012: 16-42.
    [25]
    李景云, 马越, 孙自镛, 等. 聚类分析在大肠埃希菌耐药性研究中的应用[J]. 中华微生物学和免疫学杂志, 2003, 23(5): 384-387. doi: 10.3760/j:issn:0254-5101.2003.05.023
    [26]
    CHELOSSI E, VEZZULLI L, MILANO A, et al. Antibiotic resistance of benthic bacteria in fish-farm and control sediments of the Western Mediterranean[J]. Aquaculture, 2003, 219(1/2/3/4): 83-97. https://www.sciencedirect.com/science/article/abs/pii/S0044848603000164
    [27]
    KUMAR P A, JOSEPH B, PATTERSON J. Antibiotic and heavy metal resistance profile of pathogens isolated from infected fish in Tuticorin, south-east coast of India[J]. Ind J Fish, 2011, 58(2): 121-125.
    [28]
    王瑞旋, 耿玉静, 王江勇, 等. 水产致病菌耐药基因的研究[J]. 海洋环境科学, 2012, 31(3): 323-328. doi: 10.3969/j.issn.1007-6336.2012.03.004
    [29]
    ZHANG R F, WANG Y L, GU J D. Identification of environmental plasmid-bearing Vibrio species isolated from polluted and pristine marine reserves of Hong Kong, and resistance to antibiotics and mercury[J]. Antonie Van Leeuwenhoek, 2006, 89(3/4): 307-315. doi: 10.1007/s10482-005-9032-z
    [30]
    曾德乾, 冯娟, 徐力文, 等. 海水养殖鱼哈维弧菌分离株的耐药谱型分析[J]. 中国水产科学, 2015, 22(1): 129-138. https://www.zhangqiaokeyan.com/academic-journal-cn_journal-fishery-sciences-china_thesis/0201261545633.html
    [31]
    王瑞旋, 王江勇, 徐力文, 等. 军曹鱼养殖水体及其肠道弧菌的耐药性研究[J]. 南方水产, 2007, 5(5): 1-6. https://www.schinafish.cn/article/id/e1418cc0-8a77-4a8c-b31a-6b692abb67e6
    [32]
    耿红, 宣莹莹, 蔡夏童, 等. 太原市2014年春节期间常规大气污染物浓度变化及聚类分析[J]. 环境科学学报, 2015, 35(4): 965-974. doi: 10.13671/j.hjkxxb.2014.0957
    [33]
    张晶, 胡学钢, 张斌. 基于蜕变关系的聚类程序测试方法[J]. 电子测量与仪器学报, 2011, 25(8): 688-694. doi: 10.3724/SP.J.1187.2011.00688
    [34]
    满其蒙, 冯娟, 区又君, 等. 15株鱼源致病性鱼诺卡氏菌的聚类分析[J]. 南方水产科学, 2013, 5(5): 86-92. doi: 10.3969/j.issn.2095-0780.2013.05.014?pageType=en
    • Related Articles

  • Related Articles

    [1]LI Junwei, HU Ruiping, CHEN Suwen, GUO Yongjian, ZHU Changbo, LI Ting, XIE Xiaoyong, SU Jiaqi. Effects of low salinity pressure on biological tissue and immunity enzymes activities of Sipunculus nudus[J]. South China Fisheries Science, 2021, 17(4): 41-48. DOI: 10.12131/20210022
    [2]GE Wanyi, LEI Lina, JIANG Xinyu, LI Xia, SUN Zhaosheng, WANG Wei, GAO Qian. cDNA cloning and expression pattern analysis of b2m from spotted sea bass (Lateolabrax maculatus)[J]. South China Fisheries Science, 2020, 16(6): 47-56. DOI: 10.12131/20200031
    [3]YU Jiaxin, WU Fang, SUN Chengfei, DONG Junjian, TIAN Yuanyuan, YE Xing. Cloning, structure analysis and expression of interferon gamma (IFN-γ) gene in Oreochromis niloticus[J]. South China Fisheries Science, 2017, 13(1): 85-93. DOI: 10.3969/j.issn.2095-0780.2017.01.011
    [4]LIANG Nan, HU Kun, LIU Tengfei, WU Yingjie, YANG Xianle. Tissue distribution and elimination of methylene blue and its metabolites in allogynogenetic crucian carps (Carassius auratus gibelio)[J]. South China Fisheries Science, 2017, 13(1): 19-25. DOI: 10.3969/j.issn.2095-0780.2017.01.003
    [5]QIU Ying, HUANG Guiju, LIU Baosuo, FAN Sigang, LI Youning, CHEN Mingqiang, YU Dahui. Cloning of GLUT1 gene from winged pearl oyster Pteria penguin and its expression in response to glucose challenge[J]. South China Fisheries Science, 2016, 12(5): 81-89. DOI: 10.3969/j.issn.2095-0780.2016.05.010
    [6]LI Yundong, ZHOU Falin, HUANG Jianhua, MA Zhenhua, YANG Qibin, QIU Lihua, FU Mingjun, CHEN Jinsong, JIANG Shigui. Cloning and expression analysis of Cathepsin L cDNA of Penaeus monodon[J]. South China Fisheries Science, 2016, 12(3): 58-66. DOI: 10.3969/j.issn.2095-0780.2016.03.008
    [7]LIU Yujiao, ZHU Huaping, LU Maixin, LIU Zhigang, CAO Jianmeng, GAO Fengying, KE Xiaoli. Effect of salinity stress on expression of PRLⅠgenes from tilapia and their distribution in different tissues[J]. South China Fisheries Science, 2014, 10(6): 51-57. DOI: 10.3969/j.issn.2095-0780.2014.06.007
    [8]LI Wuhu, REN Chunhua, HU Chaoqun, JIANG Xiao, ZHONG Ming. cDNA cloning and tissue distribution of calmodulin from sea cucumber (Stichopus monotuberculatus)[J]. South China Fisheries Science, 2014, 10(5): 75-81. DOI: 10.3969/j.issn.2095-0780.2014.05.011
    [9]ZHANG Han, JIANG Jingzhe, HE Jian, GU Lu, WANG Jiangyong. Study on distribution of hemocyanin in different tissues of Haliotis diversicolor[J]. South China Fisheries Science, 2014, 10(4): 34-38. DOI: 10.3969/j.issn.2095-0780.2014.04.006
    [10]PAN Liling, HUANG Guiju, CHENG Shuying, WANG Xiaoning, YU Dahui. cDNA cloning, characterization and challenge-based expression profiles of cathepsin D in winged pearl oyster Pteria penguin[J]. South China Fisheries Science, 2012, 8(2): 22-29. DOI: 10.3969/j.issn.2095-0780.2012.02.004

  • Cited by

    Periodical cited type(9)

    1. 王禹，段静瑶，王志龙，苏岩峰，喻佩. 水产品中杂环胺类化合物的形成及调控研究进展. 中国食品添加剂. 2024(02): 283-290 .
    2. 阳日东，倪来学，姚现琦，解万翠，车红霞，李红燕，董秀芳. 肉制品中杂环胺影响因素及检测方法的研究进展. 肉类研究. 2023(08): 52-60 .
    3. 陈敬敬，韩金花，张永胜，师希雄. 肉制品中杂环胺的研究进展. 中国食品卫生杂志. 2022(01): 168-174 .
    4. 王惠汀，孙学颖，王丹，郭进，靳烨，赵丽华. 肉制品中杂环胺类化合物形成及控制措施的研究进展. 食品研究与开发. 2022(05): 195-203 .
    5. 程轶群，雷阳，周兴虎，金福源，汪昌保，黄明. 传统肉制品中杂环胺研究进展. 食品科学. 2022(05): 316-327 .
    6. 谢洋洋，王小溪，闫文杰，赵卓，李兴民. 肉制品中杂环胺的研究进展. 食品研究与开发. 2017(15): 199-205 .
    7. 王晓龙，佟长青，赵前程，李伟. 我国输美水产品退回数据、存在问题及其解决对策. 河北渔业. 2016(01): 64-66 .
    8. 陈炎，蔡克周，杨潇，刘亚茜，潘丽军，姜绍通，陈从贵. 外源成分对肉制品中杂环胺抑制效果的研究进展. 食品科学. 2015(23): 329-333 .
    9. 王南，王桂瑛，赵莹莹，刘登勇. 高温加工肉制品中PhIP的形成与控制. 肉类研究. 2014(10): 33-36 .

    Other cited types(10)

Catalog

    Get Citation
    PDF
    XML
    Article views PDF downloads Cited by(19)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return