Volume 17 Issue 3
Jun.  2021
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ZHAO Xiaoyu, SU Haochang, XU Yu, XU Wujie, HU Xiaojuan, WEN Guoliang, CAO Yucheng, YU Zhaolong. Removal of sulphonamide resistance sul1 gene in water source and pond water by fishery oxidants in aquaculture[J]. South China Fisheries Science, 2021, 17(3): 46-53. DOI: 10.12131/20200231
Citation: ZHAO Xiaoyu, SU Haochang, XU Yu, XU Wujie, HU Xiaojuan, WEN Guoliang, CAO Yucheng, YU Zhaolong. Removal of sulphonamide resistance sul1 gene in water source and pond water by fishery oxidants in aquaculture[J]. South China Fisheries Science, 2021, 17(3): 46-53. DOI: 10.12131/20200231
shu

Removal of sulphonamide resistance sul1 gene in water source and pond water by fishery oxidants in aquaculture

  • 1.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China

  • 3.

    Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China

  • 4.

    Guangdong Guanli Marine Biology Co., Ltd., Maoming 525400, China

More Information
  • Received Date: November 04, 2020
  • Revised Date: March 07, 2021
  • Accepted Date: March 14, 2021
  • Available Online: March 24, 2021
    Graphical Abstract
    • Abstract
  • To explore the feasibility of using fishery oxidants to eliminate antibiotic resistant genes (ARGs) in aquaculture water and to control their spread, we studied the effects on ARG removal of three commonly used fishery oxidants (Sodium hypochlorite, dibromohydantoin and potassium permanganate) in water source and pond water by real-time fluorescent quantitative PCR. Results show that the concentration of sul1 gene was high in the water source and pond water (105 and 106 copies·mL−1, respectively), and the intracellular sul1 gene was the predominant form. Additionally, sodium hypochlorite had a strong effect on sul1 gene removal among all the experimental groups, and dibromohydantoin only showed a strong removal effect on sul1 gene in the water source. Potassium permanganate only showed a strong effect on the removal of extracellular sul1 gene in both water source and pond water. The results indicate that sodium hypochlorite has the best effect on ARG removal in both water source and pond water for these three common used fishery oxidants. Scientific application of sodium hypochlorite is beneficial to ARG removal and prevention in aquaticulture water.
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    • References (32)
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