Volume 21 Issue 1
Feb.  2025
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SONG Rongqun, WANG Zhenlu, GUO Xingchen, WANG Yizhou, XIONG Dongmei, YE Huan, LI Zhigang, SHAO Jian. Intestinal tissue structure, digestive enzymes, antioxidant enzymes and intestinal flora diversity between second filial generation and wild population of Brachymystax tsinlingensis Li, 1966[J]. South China Fisheries Science, 2025, 21(1): 85-95. DOI: 10.12131/20240148
Citation: SONG Rongqun, WANG Zhenlu, GUO Xingchen, WANG Yizhou, XIONG Dongmei, YE Huan, LI Zhigang, SHAO Jian. Intestinal tissue structure, digestive enzymes, antioxidant enzymes and intestinal flora diversity between second filial generation and wild population of Brachymystax tsinlingensis Li, 1966[J]. South China Fisheries Science, 2025, 21(1): 85-95. DOI: 10.12131/20240148
shu

Intestinal tissue structure, digestive enzymes, antioxidant enzymes and intestinal flora diversity between second filial generation and wild population of Brachymystax tsinlingensis Li, 1966

  • 1.

    Laboratory of Fishery Resources and Environmental Protection, College of Animal Science, Guizhou University/Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Guiyang 550025, China

  • 2.

    Special Fishes Research Institute, Guizhou University, Guiyang 550025, China

  • 3.

    College of Animal Science and Technology, Northwest A & F University, Yangling 712199, China

  • 4.

    Yangtze River Fisheries Research Institute/Key Laboratory of Freshwater Biodiversity Conservation, Ministry of Agriculture and Rural Affairs, Wuhan 430223, China

  • 5.

    Fishery Workstation of Baoji City, Baoji 721008, China

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  • Received Date: June 28, 2024
  • Revised Date: October 09, 2024
  • Accepted Date: October 20, 2024
  • Available Online: November 08, 2024
    Graphical Abstract
    • Abstract

  • In order to investigate the digestive system characteristics of subadults of Brachymystax tsinlingensis (Li, 1966) in different populations, and to provide a reference for its large-scale artificial breeding, resource conservation and utilization, we analyzed the characteristics of intestinal tissue, digestive enzymes, antioxidant enzymes and diversity of intestinal microbiota of the second filial generation of artificially bred B. tsinlingensis Li, 1966 [Bt, body length (15.40±0.82) cm, body mass (51.50±11.90) g] and the wild population in natural habitats [WBt, body length (18.17±2.57) cm, body mass (85.68±34.85) g] by histological method and 16S rRNA high-throughput sequencing techniques. The results show that the intestinal tissues of Bt and WBt had the same structure, both having intestinal glandular tissues. The thickness of the muscularis propria of WBt was significantly higher than that of Bt (p<0.05). There was no significant difference in the activity of intestinal digestive enzymes (p>0.05), and the activity of intestinal superoxide dismutase was significantly higher in Bt than in WBt (p<0.05). The dominant phyla of the intestinal flora of Bt and WBt were similar, and both of them were dominated by the phyla of Ascomycetes and thick-walled phyla. However, the dominant genera of the bacteria differed greatly. The dominant genera of the bacteria in Bt were the genera of Aeromonas, Clostridium narrowly_18 (Clostridium_sensu_stricto_18) and Novosphingobium, while the dominant genera in WBt were Yersinia and ZOR0006. BugBase phenotypic predictions showed higher abundance of gram-negative bacteria, aerobes, mobile elements, potential pathogens and parthenogenetic anaerobes in Bt, but the opposite for gram-positive and anaerobes. The results show that the intestinal development, digestive and absorptive capacity, resistance to oxidative stress, and microbial diversity of the wild population of B. tsinlingensis Li, 1966 were higher than those of the second filial generation, but the intestinal flora-induced pathogenicity for the second generation was relatively low.

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