Volume 4 Issue 3
Jun.  2008
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QI Xudong, QU Youjun. Expression of five kinds of isozyme in different tissues of Trachinotus ovatus[J]. South China Fisheries Science, 2008, 4(3): 38-42.
Citation: QI Xudong, QU Youjun. Expression of five kinds of isozyme in different tissues of Trachinotus ovatus[J]. South China Fisheries Science, 2008, 4(3): 38-42.
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Expression of five kinds of isozyme in different tissues of Trachinotus ovatus

  • 1.

    Guangdong Ocean University, Zhanjiang 524025, China

  • 2.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

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  • Received Date: January 30, 2008
  • Revised Date: March 12, 2008
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    • Abstract

  • Five isozymes (EST, ME, AST, LDH and MDH) from six tissues (muscle, heart, liver, kidney, brain and spleen) of Trachinotus ovatus were preliminarily studied using vertical polyacrylamide gradient gel electrophoresis. The results showed that the tissue specificity of these isozymes is very obvious. The expression of enzyme gene loci and the activity of isozyme varied with different tissues. The number of EST isozyme bands was 3, which was encoded by 2 gene loci. Est-1 expressed in all 6 tissues, and Est-2 only existed in liver and kidney. The number of LDH isozyme bands was 3, which was encoded by 3 gene loci. Ldh-2 only expressed in kidney. Two divisions of MDH were found from T.ovatus. It was encoded by 2 loci, and the most specific MDH was in liver. The isozyme bands of MDH3 also existed in all 6 tissues. The number of ME isozyme band was 1, which was encoded by 1 loci. AST isozyme which was encoded by two loci was only found in liver and kidney.

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  • [1]
    区又君, 李加儿. 卵形鲳鲹的早期胚胎发育[J]. 中国水产科学, 2005, 12(6): 786-789. doi: 10.3321/j.issn:1005-8737.2005.06.019
    [2]
    毕冰, 尹洪滨, 孙中武, 等. 怀头鳃的同工酶[J]. 东北林业大学学报, 2006, 34(3): 67-69. doi: 10.3969/j.issn.1000-5382.2006.03.027
    [3]
    曹伏君, 刘楚吾. 花尾胡椒鲷与胡椒鲷5种同工酶的比较研究[J]. 海洋科学, 2004, 28(1): 23-26. doi: 10.3969/j.issn.1000-3096.2004.01.007
    [4]
    李鹏飞, 刘萍, 柳学周, 等. 漠斑牙鲆引进种群同工酶的遗传多态性分析[J]. 中国水产科学, 2006, 13(1): 13-19.
    [5]
    王城辉, 李思发, 赵金良. 我国4种红鲤群体的生化遗传差异[J]. 上海水产大学学报, 2004, 13(1): 1-4. doi: 10.3969/j.issn.1004-7271.2004.01.001
    [6]
    GAO Tianxiang, LOU Dong, LIU Jinxian, et al. Genetic variation among sea bass populations[J]. Aeta Hydrobiologiea Siniea, 2004, 28(1): 71-75. doi: 10.3724/issn1000-3207-2004-1-69-1
    [7]
    胡能书, 万贤国. 同工酶技术及其应用[M]. 长沙: 湖南科技出版社, 1985: 86-12. https://xueshu.baidu.com/usercenter/paper/show?paperid=a2879165a1e8dde93ff1b62cfb459820&site=xueshu_se&hitarticle=1
    [8]
    何忠效, 张树政. 电泳[M]. 2版. 北京: 科学出版社, 1999: 284-308.
    [9]
    郭尧君. 蛋白质电泳实验技术[M]. 北京: 科学出版社, 1999: 70-80. https://xueshu.baidu.com/usercenter/paper/show?paperid=d56e3050a2e6c85d42d8c11b6a6d90ff&site=xueshu_se&hitarticle=1
    [10]
    SHALKLEE J B, ALLINDORF F W, MORIZOT D C, et al. Genetic nomenclature for protein-voding loci in fish[J]. Trans Am Fish Soc, 1990(119): 2-15. doi: 10.1577/1548-8659(1990)119<0002:GNFPLI>2.3.CO;2
    [11]
    邓思平, 刘楚吾. 赤点石斑鱼4种同工酶的组织分布及基因位点分析[J]. 浙江海洋学院学报: 自然科学版, 2004, 23(2): 103-106. doi: 10.3969/j.issn.1008-830X.2004.02.002
    [12]
    姜建国, 熊全沫, 姚汝华. 草鱼同工酶的组织分布及遗传结构分析[J]. 华南理工大学学报: 自然科学版, 1997, 25(12): 105-110. doi: 10.1007/BF02951625
    [13]
    刘文彬, 陈合格, 张轩杰. 黄颡鱼不同组织中同工酶的表达模式[J]. 激光生物学报, 2003, 12(14): 274-278. doi: 10.3969/j.issn.1007-7146.2003.04.007
    [14]
    尹绍武, 廖经球, 黄海, 等. 褐点石斑鱼不同组织4种同工酶的研究[J]. 海洋通报, 2007, 26(1): 472-474. doi: 10.3969/j.issn.1001-6392.2007.01.006
    [15]
    邓思平, 刘楚吾. 军曹鱼不同组织5种同工酶的研究[J]. 湛江海洋大学学报, 2002, 22(6): 1-5. doi: 10.3969/j.issn.1673-9159.2002.06.001
    [16]
    王信海, 蔺玉华, 姜秋俚, 等. 卡拉白鱼同工酶的研究[J]. 天津师范大学学报: 自然科学版, 2007, 27(3): 22-25. doi: 10.3969/j.issn.1671-1114.2007.03.008
    [17]
    丁少雄, 王军, 郭丰, 等. 台湾海区斜带石斑鱼群体遗传学的等位酶研究[J]. 厦门大学学报: 自然科学版, 2003, 42(5): 645-651. https://www.nstl.gov.cn/paper_detail.html?id=87df8072bcf461982609ffdffd3ed44f
    [18]
    冯秀妮, 张文兵, 麦康森, 等. 饲料中维生素B6对皱纹盘鲍幼鲍蛋白质代谢的影响[J]. 海洋科学, 2006, 30(10): 51-53. doi: 10.3969/j.issn.1000-3096.2006.10.012
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