Volume 1 Issue 4
Aug.  2005
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XIONG Xiao-yong, LAO Hai-hua, YE Xing, BAI Jun-jie. Preliminary study on recombinant cystatin protection to the myosin of silver carp (Hypophthalmichthys molitrix) in processing[J]. South China Fisheries Science, 2005, 1(4): 13-17.
Citation: XIONG Xiao-yong, LAO Hai-hua, YE Xing, BAI Jun-jie. Preliminary study on recombinant cystatin protection to the myosin of silver carp (Hypophthalmichthys molitrix) in processing[J]. South China Fisheries Science, 2005, 1(4): 13-17.
shu

Preliminary study on recombinant cystatin protection to the myosin of silver carp (Hypophthalmichthys molitrix) in processing

  • 1.

    Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China

  • 2.

    Life Science and Technology College, Shanghai Fisheries University, Shanghai 200090, China

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  • Received Date: May 12, 2005
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    • Abstract

  • The study investigated Chinese sturgeon recombinant cystatin gene expression in yeast Pichia pastoris, the inhibitory effect of product on papain, and the trend of myosin′s content change in processing of silver carp when cystatin were added or not. The result shows that recombinant cystatin can inhibit papain effectively, and the inhibitor curve is: y = 0.0234x + 0.0059. The result of SDS-PAGE of surimi samples were analyzed by the AlphaeaseFCTM software and it turned that the status that myosin was hydrolyzed by endogenous proteinases were relieved for 45 minutes heating time when 0.0215 mg · g-1 cystatin was added to surimi.

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    • References (10)
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