Study on acute toxicity and histopathology of copper sulfate, trichlorfon and povidone-iodine to GIFT tilapia (<i>Oreochromis niloticus</i>)

DAI Yulai; WANG Yuxi; XU Baoqing; DAI Yangxin; LIN Qicun; CAI Lijuan

doi:10.12131/20230093

In order to evaluate the reasonable dosage and safety of copper (II) sulfate, metrifonate and povidone-iodine, which are commonly used in aquaculture, and their toxic effects on GIFT tilapia (Oreochromis niloticus), we tested their acute toxicity and histopathological effects on GIFT tilapia under hydrostatic conditions. The results show that the LC₅₀s (24, 48 and 96 h) of copper sulfate were 13.715, 10.351 and 5.896 mg∙L⁻¹, respectively, and the safety concentration was 1.769 mg∙L⁻¹. The LC₅₀s (24, 48 and 96 h) of trichlorfon were 43.036, 25.887 and 16.689 mg∙L⁻¹, respectively, and the safety concentration was 2.810 mg∙L⁻¹. The LC₅₀s (24, 48 and 96 h) of povidone iodine were 10.509, 9.301 and 8.674 mg∙L⁻¹, respectively, and the safety concentration was 2.190 mg∙L⁻¹. Histopathological observation suggests that copper sulfate exposure can cause blurred gill filament outline, vacuolation of gill tissue, enlargement of liver tissue gap, vacuolation and nuclear atrophy, damage of nerve fibers in brain tissue, cell apoptosis, damage of renal tubular structure and nuclear membrane. Trichlorfon exposure can cause gill tissue damage and cell apoptosis, liver tissue denuclearization, mitochondrial swelling and vacuolation, brain tissue nerve fiber damage, nuclear pyknosis and renal interstitial hemorrhage. Exposure to povidone iodine can cause atrophy of gill filaments, incomplete structure of mitochondrial bilayer membrane, vacuolation of liver tissue, apoptosis of cells, and slight damage of nerve fibers. In conclusion, copper sulfate, trichlorfon and povidone iodine are relatively safe drugs for tilapia, but their dosage should be carefully controlled to avoid irreversible tissue damage.

[1]	颜子渊, 邓真德, 张竞, 等. 环境铜离子对吉富罗非鱼生长性能、脂代谢、肝胰脏和脾脏组织的影响[J]. 水生生物学报, 2022, 46(11): 1631-1641.
[2]	祝璟琳, 邹芝英, 李大宇, 等. 四个罗非鱼选育品种抗链球菌病能力差异研究[J]. 水生生物学报, 2017, 41(6): 1232-1241.
[3]	刘伟, 蒋明, 吴凡, 等. 饲料能量密度和投喂水平对吉富罗非鱼生长和健康的影响[J]. 水生生物学报, 2022, 46(1): 58-68.
[4]	REVITA S, ADI B, MARINA E, et al. Temporal trends of tilapia lake virus disease in Israel, 2017-2018[J]. Transbound Emerg Dis, 2020, 68(6): 3025-3033.
[5]	ZHANG D F, GAO Y X, KE X L, et al. Bacillus velezensis LF01: in vitro antimicrobial activity against fish pathogens, growth performance enhancement, and disease resistance against streptococcosis in Nile tilapia (Oreochromis niloticus)[J]. Appl Microbiol Biot, 2019, 103(21/22): 9023-9035. doi: 10.1007/s00253-019-10176-8
[6]	NICHOLSON P, MONON N, JAEMWIMOL P, et al. Coinfection of tilapia lake virus and Aeromonas hydrophila synergistically increased mortality and worsened the disease severity in tilapia (Oreochromis spp. )[J]. Aquaculture, 2020, 520: 734746. doi: 10.1016/j.aquaculture.2019.734746
[7]	AMPHAN S, UNAJAK S, PRINTRAKOON C, et al. Feeding-regimen of β-glucan to enhance innate immunity and disease resistance of Nile tilapia, Oreochromis niloticus Linn., against Aeromonas hydrophila and Flavobacterium columnare[J]. Fish Shellfish Imm, 2019, 87: 120-128. doi: 10.1016/j.fsi.2018.12.062
[8]	MARINANNA V R, JULIANA F C, FRANCISCO B G, et al. Sinuolinea niloticus n. sp., a myxozoan parasite that causes disease in Nile tilapia (Oreochromis niloticus)[J]. Parasitol Res, 2016, 115(11): 4307-4316. doi: 10.1007/s00436-016-5214-z
[9]	PÁDUA S B, FILHO R N M, MARTINS M L, et al. A survey of epitheliocystis disease in farmed Nile tilapia (Oreochromis niloticus Linnaeus, 1758) in Brazil[J]. J Appl Ichthyol, 2015, 31(5): 927-930. doi: 10.1111/jai.12840
[10]	HARRISON C K, SHIMAE A, ESTHER M, et al. Genetic parameters for black spot disease (diplopstomiasis) caused by Uvulifer sp. infection in Nile tilapia (Oreochromis niloticus L.)[J]. Aquaculture, 2020, 532: 736039.
[11]	TAVARES-DIAS M, MARCHIORI N D C, MARTINS M L. Paratrichodina africana (Ciliophora: Trichodinidae) of wild and cultured Nile tilapia in the Northern Brazil Paratrichodina africana (Ciliophora: Trichodinidae) de tilápia do Nilo selvagem e cultivada no Norte do Brasil[J]. Rev Bras Parasitol V, 2018, 22(2): 248-252.
[12]	AGUSTINA S S, TASRUDDIN, LAYOO N. The use of concentration of piper betle leaf extracts in commercial feed to parasites intensity of Trichodina sp. that attacked tilapia (Oreochromis niloticus)[J]. J Phy, 2019, 1179: 012182.
[13]	XU W N, LIU W B, LIU Z P. Trichlorfon-induced apoptosis in hepatocyte primary cultures of Carassius auratus gibelio[J]. Chemosphere, 2009, 77(7): 895-901. doi: 10.1016/j.chemosphere.2009.08.043
[14]	VARANKA Z, ROJIK I, VARANKA I, et al. Biochemical and morphological changes in carp (Cyprinus carpio L.) liver following exposure to copper sulfate and tannic acid[J]. Comp Biochem Phys C, 2001, 467-478.
[15]	HEDAYATI A, DARABITABAR F, BAGHERI T, et al. Histopathological impairment of common carp (Cyprinus carpio) induced through povidone-iodine exposure[J]. Microsc Res Techniq, 2018, 81(11): 1257-1260. doi: 10.1002/jemt.23131
[16]	缪成贤, 张亚群, 闫振龙, 等. 毒性试验组织病理学结果有害作用判断概述[J]. 药物评价研究, 2022, 45(8): 1480-1486.
[17]	周永欣, 章宗涉. 水生生物学毒性试验方法[M]. 北京: 农业出版社, 1989: 79-83.
[18]	明道绪. 兽医统计方法[M]. 成都: 成都科技大学出版社, 1991: 200-203.
[19]	赵巧雅. 重金属铜对斑马鱼鳃的毒性作用研究[D]. 南京: 南京农业大学, 2017: 27-31.
[20]	李天才, 刘小帅, 刘明昌, 等. 硫酸铜对不同规格短须裂腹鱼苗的急性毒性研究[J]. 南方农业学报, 2021, 52(3): 837-846.
[21]	AARDT W J V, HOUGH M. The effect of short-term Cu exposure on the oxygen consumption and Cu accumulation of mudfish (Labeo capensis) and the largemouth bass (Micropteris salmoides) in hard water[J]. Water SA, 2007, 33(5): 701-708.
[22]	JOOYANDEH F, SADEGHPOUR A, KHARA H, et al. Histopathological and bacterial study of skin and gill of grass carp, Ceteopharyngodon idella, (Valenciennes 1844) exposed to copper sulfate and potassium permanganate[J]. J Parasit Dis, 2016, 40(3): 1009-1013. doi: 10.1007/s12639-014-0625-1
[23]	胡亚东. 纳米铜和硫酸铜暴露对暗纹东方鲀组织病变及内质网应激的影响[D]. 南京: 南京师范大学, 2020: 51.
[24]	马莉芳. 水体铜对黄河鲤毒性的研究[D]. 郑州: 河南农业大学, 2013: 15-16.
[25]	FENG T, LI Z B, GUO X Q, et al. Effects of trichlorfon and sodium dodecyl sulphate on antioxidant defense system and acetylcholinesterase of Tilapia nilotica in vitro[J]. Pestic Biochem Phys, 2008, 92(3): 107-113. doi: 10.1016/j.pestbp.2007.10.002
[26]	辛桂瑜, 侯小露, 梁敏敏, 等. 敌百虫对奥尼罗非鱼幼鱼的急性毒性及安全浓度评价[J]. 水产科技情报, 2021, 48(1): 45-50.
[27]	吴中华. 晶体敌百虫对黄鳝肝组织、酶活性及相关去毒基因表达的影响[D]. 南昌: 江西农业大学, 2014: 10-21.
[28]	CARDOSO I, SOARES M, ANGELIS C, et al. Physiological and biochemical responses of Nile tilapia (Oreochromis niloticus) to acute trichlorfon exposure[J]. Int Aquat Res, 2020, 12(4): 243-253.
[29]	WANG X F, CHANG X L, ZHAO L F, et al. Trichlorfon exposure in common carp (Cyprinus carpio L.) leads to oxidative stress, neurotoxicity, and immune responses[J]. Aquaculture, 2022, 548: 737681. doi: 10.1016/j.aquaculture.2021.737681
[30]	XU W N, LIU W B, SHAO X P, et al. Effect of trichlorfon on hepatic lipid accumulation in crucian carp Carassius auratus gibelio[J]. J Aquat Anim Health, 2012, 24(3): 185-194. doi: 10.1080/08997659.2012.675937
[31]	CHANG X L, WANG X F, FENG J C, et al. Impact of chronic exposure to trichlorfon on intestinal barrier, oxidative stress, inflammatory response and intestinal microbiome in common carp (Cyprinus carpio L.)[J]. Environ Pollut, 2020, 259: 113846. doi: 10.1016/j.envpol.2019.113846
[32]	TAWEEL A, SHUHAIMI-OTHMAN M, AHMAD A K. In vivo acute toxicity tests of some heavy metals to tilapia fish (Oreochromis niloticus)[J]. J Biol Sci, 2013, 13(5): 365-371. doi: 10.3923/jbs.2013.365.371
[33]	李燕华, 王梦杰, 马本贺, 等. 3种常用渔药对攀鲈幼鱼的急性毒性试验[J]. 水产科技情报, 2021, 48(2): 94-98.
[34]	OWARI N B, 马晶晶, 王鸣, 等. 三种常用药物对黑鲷幼鱼的急性毒性试验[J]. 饲料工业, 2010, 31(16): 45-46.
[35]	陈罗明, 凌去非, 魏宾, 等. 四种常用鱼药对梭鲈鱼种的急性毒性实验[J]. 农业环境科学学报, 2006, 25: 492-495.
[36]	孙博, 刘琦, 裘丽萍, 等. 2种渔药对吉富罗非鱼幼鱼急性毒性研究[J]. 环境科学与技术, 2015, 38(11): 125-129.
[37]	施钢, 陈刚, 张健东, 等. 4种水产药物对蓝点笛鲷幼鱼急性毒性试验[J]. 南方水产科学, 2011, 7(3): 50-55.
[38]	罗刚, 许建和, 滕亚娟. 五种药物对梭鱼鱼种的急性毒性试验[J]. 水产科技情报, 2012, 39(1): 42-45.
[39]	SILVA A F D, CRUZ C D, REZENDE F R L D, et al. Copper sulfate acute ecotoxicity and environmental risk for tropical fish[J]. Acta Sci-Biol Sci, 2014, 36(4): 377-381. doi: 10.4025/actascibiolsci.v36i4.18373
[40]	周剑, 杜军, 刘光迅, 等. 4种常用药物对长薄鳅幼鱼的急性毒性试验研究[J]. 西南农业学报, 2012, 25(5): 1920-1924.
[41]	张俊杰, 鄢庆枇, 李胜忠, 等. 聚维酮碘和二氧化氯对泥鳅的急性毒性试验[J]. 水产科学, 2010, 29(12): 729-731.
[42]	WOO S J, CHUNG J K. Effects of trichlorfon on oxidative stress, neurotoxicity, and cortisol levels in common carp, Cyprinus carpio L., at different temperatures[J]. Comp Biochem Phys C, 2020, 229: 108698.

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Study on acute toxicity and histopathology of copper sulfate, trichlorfon and povidone-iodine to GIFT tilapia (Oreochromis niloticus)

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Study on acute toxicity and histopathology of copper sulfate, trichlorfon and povidone-iodine to GIFT tilapia (Oreochromis niloticus)

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