Volume 19 Issue 6
Dec.  2023
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DAI Yulai, WANG Yuxi, XU Baoqing, DAI Yangxin, LIN Qicun, CAI Lijuan. Study on acute toxicity and histopathology of copper sulfate, trichlorfon and povidone-iodine to GIFT tilapia (Oreochromis niloticus)[J]. South China Fisheries Science, 2023, 19(6): 116-126. DOI: 10.12131/20230093
Citation: DAI Yulai, WANG Yuxi, XU Baoqing, DAI Yangxin, LIN Qicun, CAI Lijuan. Study on acute toxicity and histopathology of copper sulfate, trichlorfon and povidone-iodine to GIFT tilapia (Oreochromis niloticus)[J]. South China Fisheries Science, 2023, 19(6): 116-126. DOI: 10.12131/20230093
shu

Study on acute toxicity and histopathology of copper sulfate, trichlorfon and povidone-iodine to GIFT tilapia (Oreochromis niloticus)

  • Hangzhou Academy of Agricultural Sciences, Hangzhou 310024, China

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  • Received Date: April 29, 2023
  • Revised Date: July 12, 2023
  • Accepted Date: July 31, 2023
  • Available Online: August 08, 2023
    Graphical Abstract
    • Abstract

  • In order to evaluate the reasonable dosage and safety of copper (II) sulfate, metrifonate and povidone-iodine, which are commonly used in aquaculture, and their toxic effects on GIFT tilapia (Oreochromis niloticus), we tested their acute toxicity and histopathological effects on GIFT tilapia under hydrostatic conditions. The results show that the LC50s (24, 48 and 96 h) of copper sulfate were 13.715, 10.351 and 5.896 mg∙L−1, respectively, and the safety concentration was 1.769 mg∙L−1. The LC50s (24, 48 and 96 h) of trichlorfon were 43.036, 25.887 and 16.689 mg∙L−1, respectively, and the safety concentration was 2.810 mg∙L−1. The LC50s (24, 48 and 96 h) of povidone iodine were 10.509, 9.301 and 8.674 mg∙L−1, respectively, and the safety concentration was 2.190 mg∙L−1. Histopathological observation suggests that copper sulfate exposure can cause blurred gill filament outline, vacuolation of gill tissue, enlargement of liver tissue gap, vacuolation and nuclear atrophy, damage of nerve fibers in brain tissue, cell apoptosis, damage of renal tubular structure and nuclear membrane. Trichlorfon exposure can cause gill tissue damage and cell apoptosis, liver tissue denuclearization, mitochondrial swelling and vacuolation, brain tissue nerve fiber damage, nuclear pyknosis and renal interstitial hemorrhage. Exposure to povidone iodine can cause atrophy of gill filaments, incomplete structure of mitochondrial bilayer membrane, vacuolation of liver tissue, apoptosis of cells, and slight damage of nerve fibers. In conclusion, copper sulfate, trichlorfon and povidone iodine are relatively safe drugs for tilapia, but their dosage should be carefully controlled to avoid irreversible tissue damage.

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