Volume 13 Issue 6
Dec.  2017
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TIAN Yuanyuan, JIAO Zhenzhen, DONG Junjian, SUN Chengfei, MA Dongmei, YE Xing. Cloning and expression analysis of jam-a genes in grass carp (Ctenopharyngodon idellus)[J]. South China Fisheries Science, 2017, 13(6): 30-40. DOI: 10.3969/j.issn.2095-0780.2017.06.004
Citation: TIAN Yuanyuan, JIAO Zhenzhen, DONG Junjian, SUN Chengfei, MA Dongmei, YE Xing. Cloning and expression analysis of jam-a genes in grass carp (Ctenopharyngodon idellus)[J]. South China Fisheries Science, 2017, 13(6): 30-40. DOI: 10.3969/j.issn.2095-0780.2017.06.004
shu

Cloning and expression analysis of jam-a genes in grass carp (Ctenopharyngodon idellus)

  • 1.

    Key Lab. of Tropical & Subtropical Fisheries Resource Application & Cultivation, Ministry of Agriculture; Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

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  • Received Date: May 09, 2017
  • Revised Date: June 11, 2017
  • Accepted Date: June 25, 2017
    Graphical Abstract
    • Abstract

  • We cloned three grass carp (Ctenopharyngodon idellus) gcjam-a genes (gcjam-a1, gcjam-a2 and gcjam-a3) and used qRT-PCR to analyze their expression pattern in nine tissues of grass carp, as well as their expression after grass carp reovirus-GD108 strain infection. The three gcjam-a genes had 294~295 amino acids and two immunoglobulin Ig domains, including typical receptor binding domain D1 and transmembrane domain D2. The D2 domains of gcjam-a genes were identical while D1 domains were slightly different. In nine tissues of healthy grass carps, gcjam-a1 was mainly expressed in blood and brain, and gcjam-a2 was mainly expressed in the gills, but gcjam-a3 could not be detected in all tested tissues. After the virus infection, gcjam-a1, gcjam-a2 and gcjam-a3 increased in gills significantly; only gcjam-a1 increased in liver, spleen, intestine, kidney and brain. The gcjam-a1, expressed highest in healthy grass carps tissues among the three gcjam-a genes, increased in all six tissues after the virus infection, which suggests that it might be the main receptor of GCRV-GD108.

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