Expression analysis of gcngr1 gene in grass carp (Ctenopharyngodon idellus) PSF cells

The Nogo-66 receptor (ngr1), expresses in neuron surface, can inhibit the regeneration of injury central nervous system (CNS) and regulate axon plasticity in mammals. Recent studies have shown that it also serves as a mammalian reovirus (MRV) neuron receptor. Grass carp reovirus (GCRV) can invade grass carp (Ctenopharyngodon idellus) and cause high mortality, so it would be helpful to understand the mechanisms of pathogenicityis on GCRV receptor. In this study, the grass carp ngr1 (gcngr1) cDNA was cloned from grass carp snout fibroblasts cells (PSF), which shared similar sequence with MRV receptor Ngr1;qRT-PCR was performed to detect the expression of gcngr1 and virus proliferation in grass carp snout fibroblasts (PSF) cells challenged by GCRV-GD108. The gcngr1 expression in PSF cells showed a significant increase after being infected by GCRV-GD108, which was consistent with the trend of virus reproduction. In addition, GCRV-GD108 was incubated with polyclonal antibody of GCRV-GD108 firstly and then infected PSF cells. A significant decrease in virus reproduction was observed, and gcngr1 mRNA expression also decreased in PSF cells. The results show that the expression of gcngr1 is related with GCRV infection in PSF cells, which provides references for determining whether gcngr1 is a GCRV receptor or not.