Pathological diagnosis of <i>Pseudobagrus ussuriensis</i> skin ulcer

HUANG Xiaoli; WANG Kaiyu; FAN Wei; DUAN Yajiao; DUAN Jing; DENG Yongqiang; DU Zongjun; GENG Yi; CHEN Defang

doi:10.3969/j.issn.2095-0780.2016.03.014

To determine the etiology of Pseudobagrus ussuriensis with skin ulcer, bacteriology was conducted from the edge of ulcer, the spleen and the kidney. Besides, wet mounts from gill, skin mucus and intestinal content were checked to exclude parasites. Pathological characteristics, target organs and visible pathogens were determined by histopathology. The results show that BHI is not a good medium for the first time bacteria isolation and culture from fish. No obvious bacteria were seen on the medium. The target organs were skin and muscle, spleen, head kidney and trunk kidney. All the fish mainly expressed necrotizing ulcerative dermatitis and panniculitis, splenitis and interstitial nephritis. Moreover, mild focal branchitis was also found. No obvious change was obversed in eye, brain, liver, heart, digestive tract and gas bladder. After re-staining the slides with Gimsa, we found long filamentous and hair-like bacteria in muscle tissue. Furthermore, fungal hyphae were also seen in muscle tissue. The etiology of this case was considered a co-infection of bacteria and fungi. The findings support the importance of histopathology in diagnosis.

[1]	黄钧, 温华成, 施金谷, 等. 黄颡鱼体表溃疡病病原菌分离鉴定及药敏试验[J]. 南方农业学报, 2012, 43(1): 107-112. doi: 10.3969/j:issn.2095-1191.2012.01.107
[2]	吉莉莉, 汪开毓, 肖丹, 等. 南方鲇溃疡综合症病原菌的分离与鉴定[J]. 淡水渔业, 2008, 38(3): 68-72. doi: 10.3969/j.issn.1000-6907.2008.03.013
[3]	杜宗君, 黄小丽, 汪开毓, 等. 齐口裂腹鱼溃疡病病原的分离鉴定[J]. 四川农业大学学报, 2011, 29(2): 274-279. doi: 10.3969/j.issn.1000-2650.2011.02.026
[4]	王国良, 金珊, 薛良义, 等. 海水网箱养殖鲈鱼皮肤溃疡病及其病原菌的研究[J]. 黄渤海海洋, 2000, 18(3): 1-6. https://xueshu.baidu.com/usercenter/paper/show?paperid=c74ec3f8b3cadfc2f6e2bbc28e968b31&site=xueshu_se
[5]	陈政强, 姚志贤, 茂林, 等. 半滑舌鳎皮肤溃疡病病原研究[J]. 水产学报, 2012, 36(5): 764-771. https://xueshu.baidu.com/usercenter/paper/show?paperid=1c5x0jr0jc600a8083340410b5519670&site=xueshu_se&hitarticle=1
[6]	范文辉, 黄倢, 王秀华, 等. 养殖大菱鲆溃疡症病原菌的分离鉴定及系统发育分析[J]. 微生物学报, 2005, 45(5): 665-670. doi: 10.3321/j.issn:0001-6209.2005.05.002
[7]	戴瑜来, 吕永林, 李凯, 等. 大黄鱼溃疡病的组织病理和超微病理研究[J]. 热带海洋学报, 2010, 29(5): 174-179. doi: 10.3969/j.issn.1009-5470.2010.05.028
[8]	马冬梅, 白俊杰, 邓国成, 等. 大口黑鲈溃疡综合征病毒MCP基因序列分析及PCR快速检测方法的建立[J]. 中国水产科学, 2010, 17(6): 1149-1156. https://xueshu.baidu.com/usercenter/paper/show?paperid=beb446c5e7a1f73edcd8de305ef3a010&site=xueshu_se&hitarticle=1
[9]	谭爱萍, 邹为民, 姜兰, 等. 黄喉拟水龟体表溃疡病原菌SG24的分类鉴定[J]. 广东海洋大学学报, 2007, 27(3): 64-68. doi: 10.3969/j.issn.1673-9159.2007.03.014
[10]	张季, 潘连德. 灰额刺尾鱼溃疡病病原分离及鉴定[J]. 上海海洋大学学报, 2012, 21(1): 48-53. https://xueshu.baidu.com/usercenter/paper/show?paperid=2f55145c81fe1bca32a48da61f7acc01&site=xueshu_se&hitarticle=1
[11]	陈志胜, 吕军仪, 吴金英, 等. 杂色鲍Haliotis diversicolor溃疡症病原菌的研究[J]. 热带海洋, 2000, 19(3): 72-77. doi: 10.3969/j.issn.1009-5470.2000.03.011
[12]	胡健饶, 章丽梅, 史雨红, 等. 一株鱼溃疡致病菌的分离及鉴定[J]. 杭州师范大学学报(自然科学版), 2011, 10(5): 444-465. doi: 10.3969/j.issn.1674-232X.2011.05.011
[13]	张玉贵, 胡波, 黄小丽, 等. 杂交鲟体表溃疡病的病原及药敏研究[J]. 长江大学学报(自然科学版), 2011, 8(6): 244-247. https://xueshu.baidu.com/usercenter/paper/show?paperid=5f3d7bbada092935a170b60e9e2599ea&site=xueshu_se&hitarticle=1
[14]	徐进, 罗晓松, 曾令兵. 黄颡鱼鲇爱德华氏菌的分离鉴定及其致病性研究[J]. 淡水渔业, 2009, 39(6): 47-53. doi: 10.3969/j.issn.1000-6907.2009.06.008
[15]	RUTH F. Mycobacterial infections of fish[J]. Southern Regional Aquaculture Center, 2011 (4706): 1-12. https://xueshu.baidu.com/usercenter/paper/show?paperid=b3fb012ca96bf0cf9c98b4f300031c3f&site=xueshu_se&hitarticle=1
[16]	WIKLUND T, BYLUND G. Skin ulcer disease of flounder Platichthys flesus in the northern Baltic Sea[J]. Dis Aquat Org, 1993, 17: 165-174. doi: 10.3354/dao017165
[17]	LUNDER T, EVENSEN O, HOLSTAD G, et al. 'Winter ulcer' in the Atlantic salmon Salmo salar. Pathological and bacteriological investigations and transmission experiments[J]. Dis Aquat Org, 1995, 23(1): 39-49. doi: 10.3354/dao023039
[18]	汪开毓, 陆艳, 耿毅, 等. 维里纳气单胞菌致泥鳅溃疡病的病理学观察[J]. 水利渔业, 2008, 28(4): 117-120. https://xueshu.baidu.com/usercenter/paper/show?paperid=9d96d5ceffdc1306ca19298ba24dba20&site=xueshu_se&hitarticle=1
[19]	潘耀谦. 动物病理学的过去、现在和未来[J]. 动物医学进展, 2000, 21(3): 1-6. https://xueshu.baidu.com/usercenter/paper/show?paperid=d709ca711ddbae267abb51b7f2d019d3&site=xueshu_se&hitarticle=1
[20]	杨燕初, 孟冬月. 病理学[M]. 北京: 人民军医出版社, 2010: 1-5.
[21]	FEIST S W, STENTIFORD G D, KENT M L, et al. Histopathological assessment of liver and gonad pathology in continental slope fish from the northeast atlantic ocean[J]. Mar Environ Res, 2015, 106: 42-50. doi: 10.1016/j.marenvres.2015.02.004
[22]	SHILTON C, BROWN G P, CHAMBERS L, et al. Pathology of runting in farmed saltwater crocodiles (Crocodylus porosus) in Australia[J]. Veter Pathol, 2014, 51(5): 1022-1034. doi: 10.1177/0300985813516642
[23]	AAMRI F E, CABALLERO M J, REAL F, et al. Streptococcus iniae in gilthead seabream (Sparus aurata, L. ) and red porgy (Pagrus pagrus, L. ): itrastructural analysis[J]. Veter Pathol, 2015, 52(1): 209-212. doi: 10.1177/0300985814520638
[24]	MITCHELL S O, STEINUM T, RODGER H, et al. Epitheliocystis in atlantic salmon, Salmo salar L., farmed in fresh water in Ireland is associated with 'Candidatus Clavochlamydia salmonicola' infection[J]. J Fish Dis, 2010, 33(8): 665-673. doi: 10.1111/j.1365-2761.2010.01171.x
[25]	沈平, 王洪印. 鱼类养殖的新品种乌苏里拟鲿[J]. 渔业致富指南, 2009(7): 7. https://xueshu.baidu.com/usercenter/paper/show?paperid=1cd46fbb2a3aec71f00ff7b67f1045a9&site=xueshu_se&hitarticle=1
[26]	何华敏. 乌苏里拟鲿养殖生物学研究[D]. 苏州: 苏州大学, 2011: 9.
[27]	MICHAEL J, MAUEL D M. Piscirickettsiosis and piscirickettsiosis like infections in fish: a review[J]. Vet Microbiol, 2002, 87(4): 279-289. https://xueshu.baidu.com/usercenter/paper/show?paperid=de442ea8f47d05d03699a88218cf920c&site=xueshu_se&hitarticle=1
[28]	耿毅, 汪开毓, 李成伟, 等. 养殖大鲵蛙病毒自然感染的病理形态学观察[J]. 中国兽医学报, 2011, 31(11): 1640-1644. https://xueshu.baidu.com/usercenter/paper/show?paperid=bd7a8e3147caffc245d65070cfd281eb&site=xueshu_se&hitarticle=1
[29]	PRADHAN P K, RATHORE G, SOOD N, et al. Emergence of epizootic ulcerative syndrome: large-scale mortalities of cultured and wild fish species in Uttar Pradesh, India[J]. Curr Sci, 2014, 106(12): 1711-1718. https://xueshu.baidu.com/usercenter/paper/show?paperid=f0c335ffa8f373854930d98695a62b0e&site=xueshu_se&hitarticle=1
[30]	WOO P T K, BRUNO D W. Fish disease and disorders. Volume 3 Viral, Bacterial and Fungal infection[M]. 2nd ed. London: CABI Publishing, 2011: 397-631.
[31]	THOMAS P, LOCHA M F. Emerging flavobacterial infections in fish: a review[J]. J Adv Res, 2015, 6(3): 283-300. https://xueshu.baidu.com/usercenter/paper/show?paperid=615d7d5ddb4207e6654527de6ba3ba12&site=xueshu_se&hitarticle=1

Pathological diagnosis of Pseudobagrus ussuriensis skin ulcer

Abstract

References

Related Articles

Catalog

Related

[1]	FAN Wei, CHEN Mengzhu, GUO Xianghui, GENG Yi, CHEN Defang, OUYANG Ping, HUANG Xiaoli, LAI Weimin, WU Jun. Diagnostic and pathological studies of epizootic ulcerative syndrome (EUS) outbreak in cultured leucistic Channa argus in Sichuan Province of China[J]. South China Fisheries Science, 2023, 19(4): 168-173. DOI: 10.12131/20220321
[2]	XIE Yundan, FENG Juan, LIU Chan, DENG Yiqin, WANG Jiangyong, SU Youlu. Comparative pathological study of tilapia naturally infected with Streptococcus agalactiae and virulence gene profiling of isolated strains[J]. South China Fisheries Science, 2019, 15(2): 47-57. DOI: 10.12131/20180185
[3]	YU Lujun, MIAO Zongyu, CAI Lei, WEI Yuanzheng, HUANG Ren, LI Jianjun. Pathogenicity and impact factors of pathogen causing ulcer disease on Mugilogobius chulae[J]. South China Fisheries Science, 2018, 14(5): 45-52. DOI: 10.3969/j.issn.2095-0780.2018.05.006
[4]	GU Liangbin, XU Liwen, FENG Juan, SU YouLu, LIU Guangfeng, GUO Zhixun. Identification and drug sensitive test of bacterial Pathogens from Plectropomus leopardus with tail fester disease[J]. South China Fisheries Science, 2015, 11(4): 71-80. DOI: 10.3969/j.issn.2095-0780.2015.04.011
[5]	WANG Jiangyong, WANG Ruixuan, SU Youlu, WU Kaichang, GUO Zhixun, JIANG Jingzhe, LIU Guangfeng, ZHAO Wang. Pathogen and pathology of "acute death syndrome" of Babylonia areolata[J]. South China Fisheries Science, 2013, 9(5): 93-99. DOI: 10.3969/j.issn.2095-0780.2013.05.015
[6]	LU Yishan, JIAN Jichang, WU Zaohe. Study on histopathology of myxosporadiosis caused by Myxobolus rotundus in crucian carp Carassius auratus auratus[J]. South China Fisheries Science, 2011, 7(3): 68-71. DOI: 10.3969/j.issn.2095-0780.2011.03.012
[7]	LI Bin, TANG Yi, WANG Zhigan, ZHANG Qiong. Control effect of Chinese herbal formula on grass carp hepatobiliary syndrome[J]. South China Fisheries Science, 2011, 7(2): 35-41. DOI: 10.3969/j.issn.2095-0780.2011.02.006
[8]	WANG Jiangyong, SUN Xiuxiu, WANG Ruixuan, SU Youlu. Isolation, identification and phylogenetic analysis of pathogen from Haliotis diversicolor Reeve with withering syndrome[J]. South China Fisheries Science, 2010, 6(5): 21-26. DOI: 10.3969/j.issn.1673-2227.2010.05.004
[9]	MA Rufei, HUANG Changjiang. Pathological study on the chronic poisoning of Nonylphenol(NP) in hybrid tilapia(Oreochromis niloticus×O.aureus)[J]. South China Fisheries Science, 2007, 3(6): 56-62.
[10]	ZHENG Tianlun, WANG Guoliang, JIN Shan, HUANG Jiaqing, HE Zhongyang, LIN Ruyi. Studies on pathogen of ulcer disease of cultured large yellow croakers, Pseudosciaena crocea and its control medicines[J]. South China Fisheries Science, 2006, 2(1): 5-10.

Pathological diagnosis of Pseudobagrus ussuriensis skin ulcer

Abstract

References

Related Articles

Catalog

Related

Export File

Citation

Format

Content