Cloning and expression analysis of <i>PmUbS</i>27 gene of black tiger shrimp (<i>Penaeus monodon</i>)

WU Song; TANG Lei; FU Mingjun; YANG Lishi; HUANG Jianhua; ZHOU Falin; JIANG Shigui

doi:10.3969/j.issn.2095-0780.2016.01.011

We screened the ubiquitin/ribosomal S27 fusion protein (PmUbS27) from hepatopancreas transcriptome data of Penaeus monodon and cloned the full-length PmUbS27 cDNA sequence by SMART-RACE technology. Besides, we studied the expression of ovaries of PmUbS27 gene at different developmental stages and in different tissues by real-time quantitative detection. The PmUbS27 cDNA was 514 base pairs (bp) long, containing a 3′-UTR of 39 nucleotides and a 465 bp open reading frame (ORF) encoding a 154 amino acid polypeptide. The PmUbS27 protein contained the conserved Ub domain and ribosome structure. The DNA-cloned gene sequence consisted of four exons and three introns. PmUbS27 mRNA was ubiquitously detected in all tested tissues, comparatively high in the ovary, hepatopancreas and lymph. The expression level of PmUbS27 mRNA was significantly higher in ovary at Stage Ⅱ and Stage Ⅴ (P < 0.05). It is showed that PmUbS27 had an important role in oogenesis of P. monodon.

[1]	李炎武, 谭卫兵, 邝雪英, 等. 泛素调节的蛋白质降解——2004年诺贝尔化学奖简介[J]. 化学教育, 2004(11): 6-7, 32. doi: 10.3969/j.issn.1003-3807.2004.11.003
[2]	KLEIGER G, MAYOR T. Perilous journey: a tour of the ubiquitin-proteasome system[J]. Trends Cell Biol, 2014, 24(6): 352-359. doi: 10.1016/j.tcb.2013.12.003
[3]	XU P, DUONG D M, SEYFRIED N T, et al. Quantitative proteomics reveals the function of unconventional ubiquitin chains in proteasomal degradation[J]. Cell, 2009, 137(1): 133-145. doi: 10.1016/j.cell.2009.01.041
[4]	NENOI M, ICHIMURA S, MITA K. Interspecific comparison in the frequency of concerted evolution at the polyubiquitin gene locus[J]. J Mol Evol, 2000, 51(2): 161-165. doi: 10.1007/s002390010076
[5]	JONES D, CANDIDO E P. Novel ubiquitin-like ribosomal protein fusion genes from the nematodes Caenorhabditis elegans and Caenorhabditis briggsae[J]. J Biol Chem, 1993, 268(26): 19545-19551. doi: 10.1016/S0021-9258(19)36550-0
[6]	ROEST H P, van KLAVEREN J, de WIT J, et al. Inactivation of the HR6B ubiquitin-conjugating DNA repair enzyme in mice causes male sterility associated with chromatin modification[J]. Cell, 1996, 86(5): 799-810. doi: 10.1016/S0092-8674(00)80154-3
[7]	IHARA M, STEIN P, SCHULTZ R M. UBE2I (UBC9), a SUMO-conjugating enzyme, localizes to nuclear speckles and stimulates transcription in mouse oocytes[J]. Biol Reprod, 2008, 79(5): 906-913. doi: 10.1095/biolreprod.108.070474
[8]	GU Y Q, CHEN Q J, GU Z, et al. Ubiquitin carboxyl-terminal hydrolase L1 contributes to the oocyte selective elimination in prepubertal mouse ovaries[J]. Acta Physiologica Sinica, 2009, 61(2): 175-184. https://pubmed.ncbi.nlm.nih.gov/19377830/
[9]	周鹏. 若干大黄鱼性腺发育相关基因的克隆与表达[D]. 厦门: 集美大学, 2009: 56-62. 10.7666/d.d161698
[10]	WANG Q, CHEN L, WANG Y, et al. Expression characteristics of two ubiquitin/ribosomal fusion protein genes in the developing testis, accessory gonad and ovary of Chinese mitten crab, Eriocheir sinensis[J]. Mol Biol Rep, 2012, 39(6): 6683-6692. doi: 10.1007/s11033-012-1474-6
[11]	戴燕彬, 韩坤煌, 颜素芬, 等. 拟穴青蟹泛素基因的克隆及其在性腺发育过程中的表达[J]. 中国水产科学, 2012, 19(6): 946-955. https://lib.cqvip.com/Qikan/Article/Detail?id=43899732
[12]	韩坤煌. 拟穴青蟹细胞周期及泛素系统若干基因参与性腺发育的研究[D]. 厦门: 集美大学, 2010: 75-80. 10.7666/d.D350631
[13]	黄建华, 马之明, 周发林, 等. 南海北部野生斑节对虾卵巢解剖结构及组织学的研究[J]. 南方水产, 2005, 1(3): 49-53. doi: 10.3969/j.issn.2095-0780.2005.03.008
[14]	陈丽丽. 中华绒螯蟹泛素融合蛋白基因的克隆及表达分析[D]. 上海: 华东师范大学, 2012: 36-53. https://cdmd.cnki.com.cn/Article/CDMD-10269-1012434236.htm
[15]	CATIC A, PLOEGH H L. Ubiquitin-conserved protein or selfish gene?[J]. Trends Biochem Sci, 2005, 30 (11): 600-604. doi: 10.1016/j.tibs.2005.09.002
[16]	FINLEY D, BARTEL B, VARSHAVSKY A. The tails of ubiquitin precursors are ribosomal-proteins whose fusion to ubiquitin facilitates ribosome biogenesis[J]. Nature, 1989, 338(6214): 394-401. doi: 10.1038/338394a0
[17]	余晓丹, 沈晓明, 颜崇淮. 锌指蛋白结构和功能研究进展[J]. 国外医学卫生学分册, 2004, 31(3): 171-175. https://d.wanfangdata.com.cn/periodical/Ch9QZXJpb2RpY2FsQ0hJTmV3UzIwMjQxMTA1MTcxMzA0EhFnd3l4LXdzeDIwMDQwMzAxMBoIcXM0OThsbWY%3D
[18]	BYSTROFF C, THORSSON V, BAKER D. HMMSTR: a hidden Markov model for local sequence-structure correlations in proteins[J]. J Mol Biol, 2000, 301(1): 173-190. doi: 10.1006/jmbi.2000.3837
[19]	OSAKA H, WANG Y L, TAKADA K, et al. Ubiquitin carboxy-terminal hydrolase L1 binds to and stabilizes monoubiquitin in neuron[J]. Hum Mol Genet, 2003, 12(16): 1945-1958. doi: 10.1093/hmg/ddg211
[20]	王莹. 中华绒整蟹类泛素化修饰(SUMOylation)途径相关基因的克隆及表达分析[D]. 上海: 华东师范大学, 2012: 52-64. http://xueweilunwen.com/doc/1667935
[21]	HOLT L J, TUCH B B, VILLEN J, et al. Global Analysis of Cdk1 substrate phosphorylation sites provides insights into evolution[J]. Science, 2009, 325(5948): 1682-1686. doi: 10.1126/science.1172867
[22]	UBERSAX J A, WOODBURY E L, QUANG P N, et al. Targets of the cyclin-dependent kinase Cdk1[J]. Nature, 2003, 425(6960): 859-864. doi: 10.1038/nature02062
[23]	DEPHOURE N, ZHOU C S, VILLÉN J, et al. A quantitative Atlas of mitotic phosphorylation[J]. Proc Natl Acad Sci, 2008, 105(31): 10762-10767. doi: 10.1073/pnas.0805139105
[24]	戴文婷, 傅明骏, 赵超, 等. 斑节对虾CDK2基因全长cDNA克隆及表达分析[J]. 南方水产科学, 2015, 11(2): 1-11. doi: 10.3969/j.issn.2095-0780.2015.02.001
[25]	YEN H C, XU Q, CHOU D M, et al. Global protein stability profiling in mammalian cells[J]. Science, 2008, 322(593): 918-923. doi: 10.1126/science.1160489
[26]	BLOOM J, CROSS F R. Multiple levels of cyclin specificity in cell-cycle control[J]. Nat Rev Mol Cell Biol, 2007, 8(2): 149-160. doi: 10.1038/nrm2105
[27]	MALUMBRES M, BARBACID M. Cell cycle, CDKs and cancer: a changing paradigm[J]. Nat Rev Cancer, 2009, 9(3): 153-166. doi: 10.1038/nrc2602
[28]	SAKAGUCHI K, KOSHIYAMA A, IWABATA K. Meiosis and small ubiquitin-related modifier (SUMO)-conjugating enzyme, Ubc9[J]. FEBS J, 2007, 274(14): 3519-3531. doi: 10.1111/j.1742-4658.2007.05905.x
[29]	涂华玉. 组蛋白H2B泛素化修饰在减数分裂过程中作用的初步研究[D]. 合肥: 中国科学技术大学, 2011: 36-41. 10.7666/d.y2029950
[30]	朱虎. 精子发生相关基因的克隆及功能研究[D]. 南京: 南京医科大学, 2002: 73-82. https://d.wanfangdata.com.cn/thesis/ChhUaGVzaXNOZXdTMjAyNDA5MjAxNTE3MjUSB1k0NTM2MTMaCHFtc2VicDNz
[31]	ZHANG F, CHEN L, WU P, et al. cDNA cloning and expression of Ubc9 in the developing embryo and ovary of Oriental river prawn, Macrobrachium nipponense[J]. Comp Biochem Physiol B, 2010, 155(3): 288-293. doi: 10.1016/j.cbpb.2009.11.013

Cloning and expression analysis of PmUbS27 gene of black tiger shrimp (Penaeus monodon)

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Cloning and expression analysis of PmUbS27 gene of black tiger shrimp (Penaeus monodon)

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