Volume 10 Issue 2
Apr.  2014
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XIAO Yayuan, XU Shannan, LIU Yong, LI Chunhou. Effect of No.0 diesel oil water-soluble fraction on CYP4 gene expression in green mussel (Perna viridis)[J]. South China Fisheries Science, 2014, 10(2): 61-66. DOI: 10.3969/j.issn.2095-0780.2014.02.009
Citation: XIAO Yayuan, XU Shannan, LIU Yong, LI Chunhou. Effect of No.0 diesel oil water-soluble fraction on CYP4 gene expression in green mussel (Perna viridis)[J]. South China Fisheries Science, 2014, 10(2): 61-66. DOI: 10.3969/j.issn.2095-0780.2014.02.009
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Effect of No.0 diesel oil water-soluble fraction on CYP4 gene expression in green mussel (Perna viridis)

  • Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; Key Lab. of Fishery Ecology Environment, Guangdong Province; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

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  • Received Date: October 20, 2013
  • Revised Date: November 24, 2013
    Graphical Abstract
    • Abstract

  • Green mussels (Perna viridis), exposed to 0 μg · L-1, 5 μg · L-1, 10 μg · L-1, 50 μg · L-1 and 100 μg · L-1 No.0 diesel oil water-soluble fraction (WSF) for 15 d under semi-hydrostatic simulation, were transferred to clean sea water for 7 d to analyze the gene expression levels of CYP4 by the method of 2-△△Ct combining with real-time quantitative PCR. Results show that WSF obviously induced CYP4 up-regulation and was differentially expressed in both visceral mass and mantle in P.viridis. The expression level of CYP4 was obviously higher in visceral mass than in mantle. The relative gene expression level initially increased then decreased and increased again as time elapsed in those two kinds of tissues. The most significant difference was observed in 50 μg · L-1concentration group (P < 0.01)compared to the control. At the last 7 d, the expression level in visceral mass and mantle decreased quickly and that in some groups gradually returned to normal level by being transferred to clean sea water.

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