Citation: | XIAO Yayuan, XU Shannan, LIU Yong, LI Chunhou. Effect of No.0 diesel oil water-soluble fraction on CYP4 gene expression in green mussel (Perna viridis)[J]. South China Fisheries Science, 2014, 10(2): 61-66. DOI: 10.3969/j.issn.2095-0780.2014.02.009 |
Green mussels (Perna viridis), exposed to 0 μg · L-1, 5 μg · L-1, 10 μg · L-1, 50 μg · L-1 and 100 μg · L-1 No.0 diesel oil water-soluble fraction (WSF) for 15 d under semi-hydrostatic simulation, were transferred to clean sea water for 7 d to analyze the gene expression levels of CYP4 by the method of 2-△△Ct combining with real-time quantitative PCR. Results show that WSF obviously induced CYP4 up-regulation and was differentially expressed in both visceral mass and mantle in P.viridis. The expression level of CYP4 was obviously higher in visceral mass than in mantle. The relative gene expression level initially increased then decreased and increased again as time elapsed in those two kinds of tissues. The most significant difference was observed in 50 μg · L-1concentration group (P < 0.01)compared to the control. At the last 7 d, the expression level in visceral mass and mantle decreased quickly and that in some groups gradually returned to normal level by being transferred to clean sea water.
[1] |
MANSUY D. The great diversity of reactions catalyzed by cytochromes P450[J]. Comp Biochem Physiol, 1998, 121(1): 5-14. doi: 10.1016/S0742-8413(98)10026-9
|
[2] |
WERCK-REICHHART D, FEYEREISEN R. Cytochromes P450: a success story[J]. Genome Biol, 2000, 1(6): 3003-3004. doi: 10.1186/gb-2000-1-6-reviews3003
|
[3] |
许华夏, 李培军, 刘宛. 生物细胞色素P450的研究进展[J]. 农业环境保护, 2002, 21(2): 188-191. doi: 10.3321/j.issn:1672-2043.2002.02.029
|
[4] |
BAUSTISTA M A M, TANAKA T. Identification of permethrin-inducible cytochrome P450s from the diamondback moth, Plutella xylostella (L) and the possibility of involvement in permethrin resistance[J]. Pestic Biochem Physiol, 2007, 8(1): 85-93. doi: 10.1016/j.pestbp.2006.06.004
|
[5] |
HOARAU P, DAMIENS G, ROMEO M, et al. Cloning and expression of a GST-pi gene in Mytilus galloprovincialis. Attempt to use the GST-pi transcript as a biomarker of pollution[J]. Comp Biochem Physiol, 2006, 143(2): 196-203. doi: 10.1016/j.cbpc.2006.02.007
|
[6] |
石玉荣, 耿建, 王惠, 等. 苯并[a]芘对宫颈癌HeLa细胞细胞色素P4501A1诱导作用[J]. 上海交通大学学报: 医学版, 2010, 30(12): 1513-1516. doi: 10.3969/j.issn.1674-8115.2010.12.016
|
[7] |
CAPDEVILA J H, FALCK J R. The CYP 450 arachidonic acid monooxygenases: from cell signaling to blood pressure regulation[J]. Biochem Biophys Res Commun, 2001, 285(3): 571-576. doi: 10.1006/bbrc.2001.5167
|
[8] |
DAVID J P, BOYER S, MESNEAU A, et al. Involvement of cytochrome P450 monooxygenases in the response of mosquito larvae to dietary plant xenobiotics[J]. Insect Biochem Mol Biol, 2006, 36(5): 410-420. doi: 10.1016/j.ibmb.2006.02.004
|
[9] |
国家海洋局. 海洋监测规范(GB 17378.1-2007)[M]北京: 海洋出版社, 2007: 153-156. https://xueshu.baidu.com/usercenter/paper/show?paperid=1f0d0690t23p08w0w94s0j80dm465256&site=xueshu_se&hitarticle=1
|
[10] |
周驰, 李纯厚, 张为民, 等. 翡翠贻贝CYP4基因克隆及其表达水平分析[J]. 热带海洋学报, 2010, 29(4): 82-88. doi: 10.3969/j.issn.1009-5470.2010.04.013
|
[11] |
LICAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real time quantitative PCR and the 2-△△Ct method[J]. Methods, 2001, 25(4): 402-408. doi: 10.1006/meth.2001.1262
|
[12] |
龙敏明, 黄桂菊, 邹记兴, 等. 育珠对合浦珠母贝N19和Prismalin-14基因表达水平的影响[J]. 南方水产科学, 2013, 9(5): 58-63. doi: 10.3969/j.issn.2095-0780.2013.05.010
|
[13] |
吴益东, 沈文飚. 棉铃虫六龄幼虫微粒体细胞色素P450和细胞色素b5的测定及组织分布[J]. 农业生物技术学报, 1997, 5(3): 297-301. https://xueshu.baidu.com/usercenter/paper/show?paperid=715b6198a4baf17dd0252ac6f8d7ad4b&site=xueshu_se
|
[14] |
陈立兵, 葛卫红, 林洁. 细胞色素P450 mRNA表达研究进展[J]. 医学研究杂志, 2007, 36(7): 79. doi: 10.3969/j.issn.1673-548X.2007.07.004
|
[15] |
徐增光, 文冠华, 高雅娟. 苯并(a)芘对小鼠肺组织细胞色素P450 1A表达的影响[J]. 华中科技大学学报: 医学版, 2005, 34(4): 404-406. doi: 10.3870/j.issn.1672-0741.2005.04.007
|
[16] |
FISHER T, CKANE M. Induction of cytochrome P450 activity in individual Chironomus riparius Meigen larvae exposed to xenobiotics[J]. Ecotoxicol Environ Saf, 2003, 54(1): 1-6. doi: 10.1016/S0147-6513(02)00031-3
|
[17] |
BURNETT K G, BAIN L J. Fundus as the premier teleost model in environmental biology: opportunities for new insights using genomics[J]. Comp Biochem Physiol, 2007, 2(4): 257-286. doi: 10.1016/j.cbd.2007.09.001
|
[18] |
周驰, 李纯厚, 张为民. 翡翠贻贝肾CYP4基因表达受多氯联苯影响的研究[J]. 安徽农业科学, 2008, 36(22): 9418-9422. doi: 10.3969/j.issn.0517-6611.2008.22.036
|
[19] |
STEGEMAN J J, SMOLOWITZ R M, HAHN M E. Immunohistochemical localization of environmentally induced cytochrome P450IAl in multiple organs of the marine teleost Stenotomus chrysops(scup)[J]. Toxicol Appl Pharmacol, 1991, 110(3): 486-504. doi: 10.1016/0041-008X(91)90049-K
|
[20] |
张薇, 宋玉芳, 孙铁珩. 菲和芘对蚯蚓(Eisenia fetida)细胞色素P450和抗氧化酶系的影响[J]. 环境化学, 2007, 26(2): 202-206. doi: 10.3321/j.issn:1672-2043.2009.02.021
|
[21] |
王磊, 宋玉芳, 张薇, 等. 蚯蚓(Eisenia fetida)细胞色素P450及抗氧化酶系对环境浓度苯并(a)芘的响应[J]. 农业环境科学学报, 2009, 28(2): 337-342. doi: 10.3321/j.issn:1672-2043.2009.02.021
|
[22] |
肖雅元, 李纯厚, 徐姗楠, 等. 水溶性石油烃组分对翡翠贻贝的毒性效应研究[J]. 农业环境科学学报, 2012, 31(3): 598-606.
|
[23] |
王燕红, 王东, 李兵, 等. 野桑蚕细胞色素P450家族CYP4M5基因的克隆和诱导表达[J]. 蚕业科学, 2009, 35(1): 78-83. doi: 10.3969/j.issn.0257-4799.2009.01.013
|
[1] | LI Wenjing, LI Chunsheng, WANG Yueqi, CHEN Shengjun, ZHAO Yongqiang, WU Yanyan, LI Laihao. Improvement effect of Halanaerobium fermentans YL9-2 on quality and flavor of fish sauce during fermentation[J]. South China Fisheries Science, 2022, 18(2): 115-123. DOI: 10.12131/20210314 |
[2] | ZHOU Kaimin, JIANG Shigui, HUANG Jianhua, YANG Qibin, JIANG Song, QIU Lihua, YANG Lishi, ZHOU Falin. Cloning and expression analysis of Chitinase-2 from Penaeus monodon during molting cycle and different larval developmental stages[J]. South China Fisheries Science, 2017, 13(4): 59-68. DOI: 10.3969/j.issn.2095-0780.2017.04.008 |
[3] | WANG Rui, LI Liping, HUANG Ting, LIANG Wanwen, LIANG Cong, LEI Aiying, CHEN Ming. Real-time quantitative PCR for detection of Streptococcus agalactiae from tilapia tissue[J]. South China Fisheries Science, 2015, 11(3): 41-46. DOI: 10.3969/j.issn.2095-0780.2015.03.007 |
[4] | QIAN Weiguo, YE Chao, WANG Weijie, LU Kexiang. Underwater irradiance and optimal allocation of 2 kW straight type fish aggregation lamps[J]. South China Fisheries Science, 2015, 11(2): 90-95. DOI: 10.3969/j.issn.2095-0780.2015.02.013 |
[5] | ZHANG Ning, SU Shengyan, DONG Zaijie, MA Qingnan, YUAN Xinhua. Study on bone and microstructure of common carp using micro CT scanning[J]. South China Fisheries Science, 2012, 8(6): 44-49. DOI: 10.3969/j.issn.2095-0780.2012.06.007 |
[6] | YU Guohui, CHEN Yanhong, CHENG Ping, LI Yongjian, YANG Zihong, CHEN Yuanfeng. Influence of several metal ions on growth and nitrite removal of Rhodopseudomonas palustris strain 2-8[J]. South China Fisheries Science, 2011, 7(4): 30-35. DOI: 10.3969/j.issn.2095-0780.2011.04.005 |
[7] | HUANG Haili, DU Xiaodong, ZHOU Yinhuan. Effects of feeding larvae and juveniles of Haliotis diversicolor with 2 benthic diatoms[J]. South China Fisheries Science, 2011, 7(1): 32-38. DOI: 10.3969/j.issn.2095-0780.2011.01.005 |
[8] | CHEN Lei, CHENG Yuanzhi, WANG Rixin, XU Tianjun. Cloning and sequence analysis of Cyt b gene in 2 Gobiidae fishes[J]. South China Fisheries Science, 2010, 6(5): 43-49. DOI: 10.3969/j.issn.1673-2227.2010.05.007 |
[9] | ZHOU Chen. Karyotype analysis of chromosome of 2 Gobioid species[J]. South China Fisheries Science, 2010, 6(4): 72-76. DOI: 10.3969/j.issn.1673-2227.2010.04.012 |
[10] | YU Da-hui, CHU ka-hou. Study on ITS 2 molecular markers of six pearl oyster species in the genus Pinctada[J]. South China Fisheries Science, 2005, 1(4): 6-12. |
1. |
曾志锋,邵伟伟,马力,陶宇慧,韦力. 棘皮动物海星(Patiria miniata)全基因组微卫星分布特征. 丽水学院学报. 2024(02): 31-39 .
![]() | |
2. |
周全,王家琪,于贵杰,吴建开,王德忠,熊阳,郭稳杰,张茂森,唐琴,梅洁. 精液添加精氨酸和亮氨酸对杂交黄颡鱼受精和孵化的影响. 水生生物学报. 2023(10): 1585-1594 .
![]() | |
3. |
范嗣刚,黄皓,王鹏飞,赵超,闫路路,邱丽华. 基于微卫星标记的花鲈亲子鉴定技术. 广东海洋大学学报. 2023(05): 26-33 .
![]() | |
4. |
杨尉,司圆圆,许瑞雯,陈兴汉. 基于基因组survey数据的疣吻沙蚕微卫星特征分析及多态标记开发. 南方水产科学. 2023(05): 123-133 .
![]() | |
5. |
刘士力,刘一诺,李飞,郑建波,程顺,蒋文枰,迟美丽,赵金良. 3种鲌亚科鱼类全基因组微卫星分布特征分析. 上海海洋大学学报. 2023(06): 1176-1183 .
![]() | |
6. |
葛健辉,关文志,任晋东,牛宝龙,胡金春,王伟,翁旭东,楼宝,于瑾,许晓军. 马口鱼全基因组简单重复序列特征分析与多态性标记开发. 浙江农业学报. 2023(11): 2584-2593 .
![]() | |
7. |
吴晓雲,陈叶雨,龚全,刘亚,宋明江,李飞扬,李鹏程,邹巧林,欧军,赖见生. 基于三代全长转录组测序的极边扁咽齿鱼SSR分子标记开发. 南方农业学报. 2023(09): 2525-2532 .
![]() | |
8. |
全德润,王李宝,黎慧,史文军,朱健强,顾舒文,顾晨,任乾,万夕和. 虾肝肠胞虫全基因组SSR位点分布及比较分析. 中国水产科学. 2023(11): 1398-1408 .
![]() | |
9. |
卢凯妹,王天骄,董世武,邢秀梅,苏伟林. 梅花鹿基因组微卫星分布特征研究. 特产研究. 2022(06): 8-15+23 .
![]() |