Volume 12 Issue 1
Feb.  2016
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LIN Quanzhuo, CHEN Yibin, HU Juan, WANG Shuqi, YANG Xiankuan, SHEN Zhuokun, ZHAO Huihong. IGF3 gene cloning and expression pattern of Protonibea diacanthus[J]. South China Fisheries Science, 2016, 12(1): 50-58. DOI: 10.3969/j.issn.2095-0780.2016.01.008
Citation: LIN Quanzhuo, CHEN Yibin, HU Juan, WANG Shuqi, YANG Xiankuan, SHEN Zhuokun, ZHAO Huihong. IGF3 gene cloning and expression pattern of Protonibea diacanthus[J]. South China Fisheries Science, 2016, 12(1): 50-58. DOI: 10.3969/j.issn.2095-0780.2016.01.008
shu

IGF3 gene cloning and expression pattern of Protonibea diacanthus

  • 1.

    College of Animal Science, South China Agricultural University, Guangzhou 510642, China

  • 2.

    Guangdong Provincial Key Lab. of Marine Biotechnology, Shantou University, Shantou 515063, China

  • 3.

    Guangdong Ocean Engineering Vocational and Technical School, Guangzhou 510320, China

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  • Received Date: December 01, 2014
  • Revised Date: March 16, 2015
    Graphical Abstract
    • Abstract

  • We cloned IGF-Ⅲ gene from Protonibea diacanthus by RACE method. The length of cDNA was 1 184 bp. The homologous analysis of deduced protein shows that the identity of IGF-Ⅲ gene and Oreochromis niloticus was 58.0%. Compared with IGF-Ⅱ and two subtypes of IGF-Ⅰof P.diacantus, the identities were 21.9%, 26.2% and 25.2%, respectively. Phylogenetic tree analysis shows that IGF-Ⅲ tended to cluster with IGF-Ⅱ firstly and then cluster with IGF-Ⅰcomposing IGF family, and finally IGF family clustered in the same branch with Insulin. Tissue distribution shows that IGF-Ⅲ was widely expressed in heart, gonad and brain of P.diacanthus, especially highest in gonad. Quantitative real-time PCR analysis reveals that the expression of IGF-Ⅲ in testis was significantly higher in developing phase than that in the others, showing a significant decline. The expression of IGF-Ⅲ in ovary of maturation phase was also remarkably higher than that in other periods. Besides, the expression of IGF-Ⅲ in forebrain, midbrain and hindbrain in female fish in mature phase was higher than that in other periods. However, IGF-Ⅲ expression pattern was different in male fish. It is suggested that IGF-Ⅲ might be important for the development of testis and maturation of ovary, and forebrain might involve in the feedback regulation of IGF-Ⅲ expression in gonad.

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