Volume 18 Issue 6
Dec.  2022
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ZHOU Nan, WANG Yakun, HONG Kunhao, WEI Jie, YU Lingyun, ZHU Xinping. Molecular cloning, expression profile and subcellular localization of nanos1 gene from Macrobrachium rosenbergii[J]. South China Fisheries Science, 2022, 18(6): 52-59. DOI: 10.12131/20220067
Citation: ZHOU Nan, WANG Yakun, HONG Kunhao, WEI Jie, YU Lingyun, ZHU Xinping. Molecular cloning, expression profile and subcellular localization of nanos1 gene from Macrobrachium rosenbergii[J]. South China Fisheries Science, 2022, 18(6): 52-59. DOI: 10.12131/20220067
shu

Molecular cloning, expression profile and subcellular localization of nanos1 gene from Macrobrachium rosenbergii

  • 1.

    School of Fishery, Zhejiang Ocean University, Zhoushan 316000, China

  • 2.

    Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of Tropical & Subtropical Fishery Resource Application & Cultivation, Ministry of Agriculture and Rural Affairs, Guangzhou 510380, China

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  • Received Date: March 14, 2022
  • Revised Date: April 17, 2022
  • Accepted Date: May 05, 2022
  • Available Online: June 04, 2022
    Graphical Abstract
    • Abstract
  • To investigate the function of the nanos1 gene in the reproductive development of Macrobrachium rosenbergii, we cloned its nanos1 gene. The cDNA sequence was 2 811 bp, encoding 243 amino acids. Phylogenetic analysis shows that it had the highest homology with Eriocheir sinensis, higher homology with nanos1 in fish but lower homology with mammals. We detected the expression level of the gene at different embryonic developmental stages and in larvae by real-time fluorescence quantitative PCR. The results show that the expression level of nanso1 mRNA was the highest at unfertilized stage, significantly higher than that at fertilized stage and the other embryonic developmental stages (P<0.05). During the embryonic development, the expression was the highest at fertilization stage, significantly higher than that at cleavage stage and very significantly higher than that at late embryonic development (P<0.01). The expression level of this gene at cleavage stage was significantly higher than that from blastocyst stage to larval stage. The expression level from blastocyst stage to larval stage was low and there was no difference.In situ hybridization shows that nanos1 mRNA was expressed in the cytoplasm of oocytes and primary oocytes (Oc1, Oc2, Oc3 and Oc4). The results show that nanos1 is closely related to the development of female germ cells of M. rosenbergii.
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    • References (36)
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