Volume 13 Issue 5
Oct.  2017
Turn off MathJax
Article Contents
Abstract
References
ZHANG Yue, DUAN Yafei, DONG Hongbiao, ZHANG Jiasong. Effects of poly-β-hydroxybutyrate (PHB) on immune and digestive indicators in hepatopancreas of Litopenaeus vannamei[J]. South China Fisheries Science, 2017, 13(5): 78-84. DOI: 10.3969/j.issn.2095-0780.2017.05.011
Citation: ZHANG Yue, DUAN Yafei, DONG Hongbiao, ZHANG Jiasong. Effects of poly-β-hydroxybutyrate (PHB) on immune and digestive indicators in hepatopancreas of Litopenaeus vannamei[J]. South China Fisheries Science, 2017, 13(5): 78-84. DOI: 10.3969/j.issn.2095-0780.2017.05.011
shu

Effects of poly-β-hydroxybutyrate (PHB) on immune and digestive indicators in hepatopancreas of Litopenaeus vannamei

  • 1.

    Key Lab. of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

More Information
  • Received Date: December 13, 2016
  • Revised Date: January 22, 2017
  • Accepted Date: March 19, 2017
    Graphical Abstract
    • Abstract

  • Litopenaeus vannamei of average weight of (5.66±0.14) g had been fed for 35 d with four different diets:0%, 1%, 3% and 5% poly-β-hydroxybutyrate (PHB) supplemented feed, and the immune and digestive indicators in hepatopancreas of shrimp were evaluated in this study.The results show that compared with the control group, the shrimps in 3% PHB group had higher total antioxidant capacity (T-AOC) and lysozyme (LSZ) activity (P < 0.05).The relative expression level of Toll gene increased but then decreased with increasing PHB content, and the expression levels in 1% and 3% PHB groups were higher than that in the control group significantly (P < 0.05).The relative expression levels of heat shock protein 70 (HSP70) gene in all three PHB treatment groups were higher than that in the control group significantly (P < 0.05).The amylase, pepsin and trypsin activities of 5% PHB group were significantly higher than those of the control group (P < 0.05), but the lipase activity showed no significant difference (P > 0.05).It is indicated that appropriate PHB content in diet can improve the immune and digestive abilities in hepatopancreas of shrimps significantly.

    • FullText(HTML)
    • References (33)
  • [1]
    李玉虎, 宋芹芹, 张志怀, 等.凡纳滨对虾生长发育规律及生长曲线拟合研究[J].南方水产科学, 2015, 11(1):89-95. http://www.schinafish.cn/CN/abstract/abstract9184.shtml
    [2]
    向赟, 吴宇学, 王刚, 等.不同温度下哈维氏弧菌和白斑综合症病毒对凡纳滨对虾的致病性[J].大连海洋大学学报, 2015, 30(1):56-61. http://edu.wanfangdata.com.cn/Periodical/Detail/hnrdnydxxb201601001
    [3]
    李晶晶, 李云, 刘红, 等.感染溶藻弧菌及白斑综合症病毒后凡纳滨对虾不同组织的Toll样受体基因表达变化研究[J].海洋与湖沼, 2016, 47(2):476-483. http://www.cqvip.com/QK/90072X/201602
    [4]
    罗词兴, 黄旭雄, 李桑, 等.溶藻弧菌感染后凡纳滨对虾鳃组织免疫相关基因的表达[J].中国水产科学, 2014, 21(1):189-196. http://www.cqvip.com/QK/90072X/201602/669133827.html
    [5]
    李健, 梁俊平, 李小彦, 等.黄芩苷在中国对虾体内对诺氟沙星消除及细胞色素P450酶的影响[J].海洋科学, 2012, 36(3):81-88. http://www.oalib.com/references/17806877
    [6]
    文国樑, 林黑着, 李卓佳, 等.饲料中添加复方中草药对凡纳滨对虾生长、消化酶和免疫相关酶活性的影响[J].南方水产科学, 2012, 8(2):58-63. http://www.schinafish.cn/CN/abstract/abstract8940.shtml
    [7]
    杨铿, 林黑着, 夏冬梅, 等.饲料中海洋红酵母对尼罗罗非鱼幼鱼生长性能、消化酶及免疫酶活性的影响[J].南方水产科学, 2016, 12(6):51-58. http://www.schinafish.cn/CN/abstract/abstract9401.shtml
    [8]
    BARUAH S K, NOROUZITALLAB P, DEBNATH D, et al.Organic acids as non-antibiotic nutraceuticals in fish and prawn feed[J].Aquacult Health Int, 2008(12):4-6. https://www.researchgate.net/publication/293336603_Organic_acids...
    [9]
    孙慧贤, 隋丽英.聚β-羟基丁酸酯对卤虫幼体的强化作用[J].上海海洋大学学报, 2011, 20(3):392-398. http://www.cnki.com.cn/Article/CJFDTOTAL-SCKX201408013.htm
    [10]
    魏茂繁, 邵云海, 钟卫鸿.生物合成聚-β-羟基丁酸(PHB)的研究现状与应用前景[J].黑龙江科学, 2014, 5(6):24-25. http://www.cnki.com.cn/Article/CJFDTOTAL-GSKJ200603049.htm
    [11]
    刘春, 张小凡.PHB在生产可降解塑料方面的应用及其微生物累积的研究进展[J].塑料工业, 2005, 33(8):1-3, 18. http://www.cnki.com.cn/Article/CJFDTotal-XJJB201432022.htm
    [12]
    邓元告, 黄琼叶, 马灌楠, 等.PHB剂量和饲喂时间对中华绒螯蟹肝胰腺酶活力和肠道菌群多样性的影响[J].中国水产科学, 2016, 23(1):138-145. http://d.wanfangdata.com.cn/Periodical_zgsckx201601013.aspx
    [13]
    De SCHRYVER P, SINHA A, KUNWAR P, et al.Poly-β-hydroxybutyrate (PHB) increases growth performance and intestinal bacterial range-weighted richness in juvenile European sea bass, Dicentrarchus labrax[J].Appl Microbiol Biotechnol, 2010, 86(5):1535-1541. doi: 10.1007/s00253-009-2414-9
    [14]
    曹俊明, 严晶, 王国霞, 等.家蝇蛆粉替代鱼粉对凡纳滨对虾消化酶、转氨酶活性和肝胰腺组织结构的影响[J].南方水产科学, 2012, 8(5):72-79. http://www.schinafish.cn/CN/abstract/abstract8983.shtml
    [15]
    邓康裕, 孔杰, 孟宪红, 等.聚β-羟基丁酸酯对中国明对虾抗WSSV能力的影响[J].水产学报, 2014, 38(8):1167-1174. http://industry.wanfangdata.com.cn/dl/Detail/Periodical?id=...
    [16]
    SUGUNA P, BINURAMESH C, ABIRAMI P, et al.Immunostimulation by poly-β hydroxybutyrate-hydroxyvalerate (PHB-HV) from Bacillus thuringiensis in Oreochromis mossambicus[J].Fish Shellfish Immunol, 2014, 36(1):90-97. doi: 10.1016/j.fsi.2013.10.012
    [17]
    NHAN D T, WILLE M, de SCHRYVER P, et al.The effect of poly-β-hydroxybuty rate on larviculture of the giant fresh water prawn Macrobrachium rosenbergii[J].Aquaculture, 2010, 302(1/2):76-81. https://www.sciencedirect.com/science/article/pii/S105046481630715X
    [18]
    刘玉, 隋丽英, 邓元告, 等.聚β-羟基丁酸酯(PHB)对中华绒螯蟹(Eriocheir sinensis)幼蟹生长和肝胰腺酶活力的影响[J].海洋与湖沼, 2013, 44(5):1333-1338. http://www.cnki.com.cn/Article/CJFDTOTAL-HYFZ201305031.htm
    [19]
    WU F, XUE Y, LIU X, et al.The protective effect of eicosapentaenoic acid-enriched phospholipids from sea cucumber Cucumaria frondosa on oxidative stress in PC12 cells and SAMP8 mice[J].Neurochem Int, 2014, 64:9-17. doi: 10.1016/j.neuint.2013.10.015
    [20]
    张克烽, 张子平, 陈芸, 等.动物抗氧化系统中主要抗氧化酶基因的研究进展[J].动物学杂志, 2007, 42(2):153-160. http://www.wenkuxiazai.com/doc/eec3817c1711cc7931b716df-4.html
    [21]
    段亚飞, 董宏标, 王芸, 等.干露胁迫对日本囊对虾抗氧化酶活性的影响[J].南方水产科学, 2015, 11(4):102-108. http://www.schinafish.cn/CN/abstract/abstract9241.shtml
    [22]
    段亚飞, 董宏标, 王芸, 等.鳗弧菌感染对斑节对虾免疫相关指标的影响[J].海洋科学, 2015, 39(9):44-50. doi: 10.11759//hykx20150409001
    [23]
    邓康裕, 孔杰, 孟宪红, 等.聚β-羟基丁酸酯对凡纳滨对虾抗WSSV能力及免疫基因表达量的影响[J].中国水产科学, 2015, 22(5):877-886. http://www.cqvip.com/QK/98270X/201505/666134560.html
    [24]
    SUI L Y, MA G N, LU W L, et al.Effect of poly-β-hydroxybutyrate on growth, enzyme activity and intestinal microbial community of Chinese mitten crab, Eriocheir sinensis (Milne-Edwards) juveniles[J].Aquacult Res, 2016, 47(11):3644-3652. doi: 10.1111/are.2016.47.issue-11
    [25]
    雷爱莹, 曾地刚.复方中草药对凡纳滨对虾热应激蛋白70基因表达的影响[J].广西农业科学, 2008, 39(6):830-833. http://www.cqvip.com/QK/83434X/200804/29012150.html
    [26]
    曹煜成, 文国樑, 张华军, 等.地衣芽孢杆菌对凡纳滨对虾Toll和HSP70基因表达的影响[J].中国微生态学杂志, 2013, 25(8):882-886. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGWS201308003.htm
    [27]
    THAI T, WILLE M, GARCIA-GONZALEZ L, et al.Poly-β-hydroxybutyrate content and dose of the bacterial carrier for Artemia enrichment determine the performance of giant freshwater prawn larvae[J].Appl Microbiol Biotechnol, 2014, 98(11):5205-5215. doi: 10.1007/s00253-014-5536-7
    [28]
    邓康裕, 孟宪红, 孔杰, 等.聚β-羟基丁酸酯对凡纳滨对虾(Litopenaeus vannamei)非特异性免疫力及氨氮胁迫后免疫基因表达量的影响[J].渔业科学进展, 2015, 36(6):71-78. doi: 10.11758/yykxjz.20150611
    [29]
    MORTENSEN F, NIELSEN H, MULVANY M, et al.Short chain fatty acids dilate isolated human colonic resistance arteries[J].Gut, 1990, 31(12):1391-1394. doi: 10.1136/gut.31.12.1391
    [30]
    PARTANEN K, MROZ Z.Organic acids for performance enhancement in pig diets[J].Nutr Res Rev, 1999, 12(1):117-145. doi: 10.1079/095442299108728884
    [31]
    NAJDEGERAMI E, TRAN T, DEFOIRDT T, et al.Effects of poly-β-hydroxybutyrate (PHB) on Siberian sturgeon (Acipenser baerii) fingerlings performance and its gastrointestinal tract microbial community[J].FEMS Microbiol Ecol, 2012, 79(1):25-33. doi: 10.1111/j.1574-6941.2011.01194.x
    [32]
    NAJDEGERAMI E H, BARUAH K, SHIRI A A, et al.Siberian sturgeon (Acipenser baerii) larvae fed Artemia nauplii enriched with poly-β-hydroxybutyrate (PHB):effect on growth performance, body composition, digestive enzymes, gut microbial community, gut histology and stress tests[J].Aquacult Res, 2015, 46(4):801-812. doi: 10.1111/are.12231
    [33]
    WELTZIEN F A, HEMRE G I, EVJEMO J O, et al.β-hydroxybutyrate in developing nauplii of brine shrimp (Artemia franciscana K.) under feeding and non-feeding conditions[J].Comp Biochem Physiol B, 2000, 125(1):63-69. doi: 10.1016/S0305-0491(99)00159-5
    • Related Articles

  • Cited by

    Periodical cited type(11)

    1. 姜晓娜，胡雪松，石潇丹，葛彦龙，李池陶，程磊，石连玉，贾智英. 不同月龄镜鲤“龙科11号”形态性状对体质量的通径分析. 水产学杂志. 2024(06): 59-65+103 .
    2. 刘康，韦玲静，张盛，莫飞龙，卢玉典，杨宾兰，甘宝江，廖愚，肖珊. 不同规格百色黑鲤的形态性状与体质量的相关性及通径分析. 江西水产科技. 2024(06): 10-15 .
    3. 刘艳超，陈锋，魏聪，李建川. 2种规格弧唇裂腹鱼幼鱼形态性状对体质量的相关性及通径分析. 安徽农学通报. 2022(06): 98-101 .
    4. 胡静，周胜杰，杨蕊，陈旭，杨其彬，马振华. 眼斑双锯鱼仔稚鱼发育异速生长. 水生生物学报. 2020(04): 844-852 .
    5. 李振通，田永胜，唐江，成美玲，马文辉，庞尊方，李文升，刘江春，王晓梅，翟介明. 云龙石斑鱼与云纹石斑鱼、珍珠龙胆石斑鱼的生长性状及对比分析. 水产学报. 2019(04): 1005-1017 .
    6. 苟盼盼，王秀利，窦冬雨，荆笛，刘圣聪，刘福君，刘海金，杨君，孟雪松，仇雪梅. 红鳍东方鲀不同家系群体的形态性状差异与相关性分析. 大连海洋大学学报. 2019(05): 674-679 .
    7. 赵旺，江淼，陈明强，林凤昊，杨蕊，于刚，孟祥君，马振华. 离岸养殖与陆基养殖尖吻鲈形态性状与体质量的相关性研究. 海洋学报. 2018(08): 53-62 .
    8. 赵旺，杨蕊，胡静，杨其彬，马振华，于刚. 5月龄斜带石斑鱼形态性状对体质量影响的通径分析. 大连海洋大学学报. 2017(05): 557-562 .
    9. 赵旺，胡静，马振华，于刚，杨蕊，王理. 尖吻鲈幼鱼形态性状对体质量影响的通径分析及生长曲线拟合. 南方农业学报. 2017(09): 1700-1707 .
    10. 赵旺，于刚，王江勇，严俊贤，杨蕊，吴开畅. 7月龄方斑东风螺形态性状对体质量的通径分析. 海洋科学. 2017(11): 82-88 .
    11. 王兰梅，朱文彬，董在杰，苏胜彦，傅建军，阎明信，刘念. 福瑞鲤选育家系不同养殖阶段的生长差异分析. 南方水产科学. 2017(01): 43-49 . 本站查看

    Other cited types(4)

Catalog

    Get Citation
    PDF
    XML
    Article views (3260) PDF downloads (669) Cited by(15)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return