Pharmacokinetics and bioavailability of sarafloxacin hydrochloride in Litopenaeus vannamei

We investigated the pharmacokinetics and bioavailability of sarafloxacin hydrochloride in Litopenaeus vannamei following intra-sinus injection (10 mgkg-1) and oral administration (30 mgkg-1) using the RP-HPLC. The shrimps were kept in seawater with salinity of 33 and at temperature of (28.01.0) ℃. The concentration-time curves of haemolymph sarafloxacin hydrochloride after intra-sinus injection and oral adminstration were best described by two-compartment model and two-compartment model with first absorption, respectively. After oral adminstration, the bioavailability (F) of sarafloxacin hydrochloride was 61.6%, and the concentration of sarafloxacin hydrochloride in hepatopancreaswas 24.4 and 51.9 times higher than that in hemolymph and muscle, respectively, while the AUC0-t of hepatopancrea was 18.7 and 62.0 times higher than that in hemolymph and muscle, respectively. However, the sarafloxacin hydrochloride was faster eliminated in hepatopancreas and muscle, the reside concentration being less than 0.1 mgkg-1 in 36 h for muscle and in 5 d for hepatopancreas after oral administration. Since sarafloxacin hydrochloride has good absorption and high bioavailability with faster elimination in hepatopancreas and muscle, it is an ideal antimicrobial agent for prevention and treatment of shrimp bacterial diseases.