Volume 19 Issue 5
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JIANG Kui, HU Xiaojuan, XU Chuangwen, HONG Minna, LIU Xiyao, MAI Xiaoyong, CHEN Haiyi, YANG Keng. Effects of schizophyllan on growth, immunity and intestinal microflora of Litopenaeus vannamei[J]. South China Fisheries Science, 2023, 19(5): 95-103. DOI: 10.12131/20230041
Citation: JIANG Kui, HU Xiaojuan, XU Chuangwen, HONG Minna, LIU Xiyao, MAI Xiaoyong, CHEN Haiyi, YANG Keng. Effects of schizophyllan on growth, immunity and intestinal microflora of Litopenaeus vannamei[J]. South China Fisheries Science, 2023, 19(5): 95-103. DOI: 10.12131/20230041
shu

Effects of schizophyllan on growth, immunity and intestinal microflora of Litopenaeus vannamei

  • 1.

    South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences/Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment, Guangzhou 510300, China

  • 2.

    Guangzhou Xinhailisheng Biotechnology Co., Ltd., Guangzhou 510300, China

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  • Received Date: March 12, 2023
  • Revised Date: June 09, 2023
  • Accepted Date: June 28, 2023
  • Available Online: July 06, 2023
    Graphical Abstract
    • Abstract

  • Schizophyllan (SPG) is a type of polysaccharide with β-(1,6) branching β-(1,3)-D-glucan, extracted from the fruiting body, mycelium or fermentation broth of Schizophyllum communer. In order to study the effects of SPG feed on the cultivation of Litopenaeus vannamei, we selected 12 L. vannamei breeding ponds, and set up four groups according to the addition amounts of SPG [Group C (0%), Group S1 (0.5%), Group S2 (1.0%) and Group S3 (2.0%)] for a 56-day experiment, then we investigated the growth, blood clearance, immune indicators and intestinal microflora. The results show that the final body mass, average weight gain rate and specific weight gain rate of Group S2 were significantly higher than those of Group C, S1 and S3 (P<0.05). The height of inner epithelial cells in Group S2 and S3 were significantly higher than those in Group C and S1 (P<0.05). Compared with Group C, the contents of serum uric acid (UC) in Group S2 and S3 were significantly lower (P<0.05), but there was no significant difference in Group S1 (P>0.05). The activities of lysozyme (LZM), total nitric oxide synthase (TNOS), phenol oxidase (PO) and alkaline phosphatase (AKP) in serum of Group S2 and S3 were significantly higher than those in Group C (P<0.05). The activities of catalase (CAT), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) in Group S2 were significantly higher than those of Group C (P<0.05). Compared with the control group, the contents of serum malondialdehyde (MDA) in all experimental groups decreased to different extents (P>0.05). There was no significant difference in Ace, Chao1, Shannon and Simpson indexes of intestinal flora (P>0.05). At phylum level, compared with Group C, the abundance of Proteobacteria in the experimental groups added with SPG decreased, while that of Tenericutes increased. At genus level, compared with Group C, the abundances of Formosa, Pseudoruegeria, Muricauda and Rugella in Group S2 increased significantly (P<0.05), while the abundance of Vibrio decreased significantly (P<0.05). In conclusion, adding 1.0% SPG in feed can improve the growth performance, immunity and antioxidant capacity of L. vannamei, increase the proportion of beneficial bacteria and reduce the proportion of harmful bacteria in intestinal tract.

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  • [1]
    文国樑, 李卓佳, 林黑着, 等. 规格与盐度对凡纳滨对虾肌肉营养成分的影响[J]. 南方水产, 2007, 3(3): 31-34.
    [2]
    李玉虎, 宋芹芹, 张志怀, 等. 凡纳滨对虾生长发育规律及生长曲线拟合研究[J]. 南方水产科学, 2015, 11(1): 89-95.
    [3]
    农业农村部渔业渔政管理局, 全国水产技术推广总站, 中国水产学会. 2023中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2023: 22, 24.
    [4]
    TZIANABOS A O. Polysaccharide immunomodulators as therapeutic agents: structural aspects and biologic function[J]. Clin Microbiol Rev, 2000, 13(4): 523-533. doi: 10.1128/CMR.13.4.523
    [5]
    张虎成, 齐贺. 发酵原料药生产[M]. 北京: 中国轻工业出版社, 2014: 238-240.
    [6]
    尚庆辉, 解玉怀, 张桂国, 等. 植物多糖的免疫调节作用及其机制研究进展[J]. 动物营养学报, 2015, 27(1): 49-58. doi: 10.3969/j.issn.1006-267x.2015.01.008
    [7]
    毛绍春, 李竹英, 李聪. 人工裂褶菌多糖结构及含量变化研究[J]. 资源开发与市场, 2007, 23(5): 385-386. doi: 10.3969/j.issn.1005-8141.2007.05.001
    [8]
    李翘楚, 张璐, 王红艳, 等. 裂褶菌胞内多糖的提取纯化及生物活性分析[J]. 食品工业科技, 2023, 44(4): 252-260.
    [9]
    刘小玲, 徐向群, 黄燕华, 等. 桦褐孔菌多糖对凡纳滨对虾生长和血清免疫相关酶活性的影响[J]. 水产科学, 2014, 33(4): 201-207.
    [10]
    昌鸣先, 陈孝煊, 吴志新, 等. 虫草多糖对日本沼虾免疫机能的影响[J]. 华中农业大学学报, 2001, 20(3): 275-278. doi: 10.3321/j.issn:1000-2421.2001.03.020
    [11]
    徐申波, 郭振, 陈师勇, 等. 灵芝多糖对凡纳滨对虾生长和免疫功能的影响[J]. 青岛农业大学学报 (自然科学版), 2021, 38(4): 290-294, 304.
    [12]
    李红权, 刘存歧, 李志英, 等. 灰树花多糖对日本对虾免疫活性的影响[J]. 水生态学杂志, 2008, 29(6): 128-131.
    [13]
    ZHAO H X, CAO J M, WANG A L, et al. Effect of long-term administration of dietary β-1, 3-glucan on growth, physiological and immune responses in Litopenaeus vannamei (Boone, 1931)[J]. Aquac Int, 2012, 20(1): 145-158. doi: 10.1007/s10499-011-9448-6
    [14]
    万安滔, 张林存, 程星宇, 等. 香菇多糖对罗非鱼非特异性免疫力的影响[J]. 江苏农业科学, 2012, 40(4): 225-227.
    [15]
    杨娜, 王鸿飞, 董栓泉, 等. 裂褶菌多糖对小鼠免疫活性作用的研究[J]. 现代食品科技, 2014, 30(8): 1-5.
    [16]
    COOK M T, HAYBALL P J, HUTCHINSON W, et al. Administration of a commercial immunostimulant preparation, EcoActiva™ as a feed supplement enhances macrophage respiratory burst and the growth rate of snapper (Pagrus auratus, Sparidae (Bloeh and Schneider)) in winter[J]. Fish Shellfish Immunol, 2003, 14(4): 333-345. doi: 10.1006/fsim.2002.0441
    [17]
    MISRA C K, DAS B K, MUKHERJEE S C, et al. Effect of long term administration of dietary β-glucan on immunity, growth and survival of Labeo rohita fingerlings[J]. Aquaculture, 2006, 255(14): 82-94.
    [18]
    AI Q H, MAI K, ZHANG L, et a1. Effects of dietary β-l, 3 glucan on innate immune response of large yellow croaker, Pseudosciaena crocea[J]. Fish Shellfish Immunol, 2007, 22(4): 394-402. doi: 10.1016/j.fsi.2006.06.011
    [19]
    董淑丽, 王占彬, 雷雪芹, 等. 热应激对动物血液生化指标的影响[J]. 家畜生态, 2004, 25(2): 54-56.
    [20]
    COMA J, CARRION D, ZIMMERMAN D R. Use of plasma urea nitrogen as a rapid response criterion to determine the lysine requirement of pigs[J]. J Anim Sci, 1995, 73(2): 472-481. doi: 10.2527/1995.732472x
    [21]
    赵军, 林英庭, 孙建凤, 等. 饲粮中不同水平浒苔对蛋鸡蛋黄品质、抗氧化能力和血清生化指标的影响[J]. 动物营养学报, 2011, 23(3): 452-458.
    [22]
    WORTMANN, ROBERT L. Gout and hyperuricemia[J]. Curr Opin Rheumatol, 2002, 14(3): 281-286. doi: 10.1097/00002281-200205000-00015
    [23]
    LI H F, XU C, ZHOU L, et al. Beneficial effects of dietary β-glucan on growth and health status of Pacific white shrimp Litopenaeus vannamei at low salinity[J]. Fish Shellfish Immunol, 2019, 91: 315-324.
    [24]
    黄健彬, 迟艳, 周传朋, 等. 褐藻寡糖对卵形鲳鲹幼鱼生长性能, 抗氧化能力和免疫功能的影响[J]. 南方水产科学, 2022, 18(3): 118-128.
    [25]
    江晓路, 杜以帅, 王鹏, 等. 褐藻寡糖对刺参体腔液和体壁免疫相关酶活性变化的影响[J]. 中国海洋大学学报, 2009, 39(6): 1188-1192.
    [26]
    王鹏, 江晓路, 江艳华, 等. 褐藻低聚糖对提高大菱鲆免疫机能的作用[J]. 海洋科学, 2006, 30(8): 6-9.
    [27]
    VALENTE L M P, BATISTA S, RIBEIRO C, et al. Physical processing or supplementation of feeds with phytogenic compounds, alginate oligosaccharide or nucleotides as methods to improve the utilization of Gracilaria gracilis by juvenile European seabass (Dicentrarchus labrax)[J]. Aquaculture, 2021, 530: 1-14.
    [28]
    JAMI M J, KENARI A A, PAKNEJAD H, et al. Effects of dietary b-glucan, mannan oligosaccharide, Lactobacillus plantarum and their combinations on growth performance, immunity and immune related gene expression of Caspian trout, Salmo trutta caspius (Kessler, 1877)[J]. Fish Shellfish Immunol, 2019, 91: 202-208.
    [29]
    ZHAO H X, CAO J M, WANG A L, et al. Effect of dietary β-1, 3-glucan on the immune response of Litopenaeus vannamei exposed to nitrite-N[J]. Aquac Nutr, 2012, 18(3): 272-280. doi: 10.1111/j.1365-2095.2011.00893.x
    [30]
    SORAAT A, SASIMANAS U, CHEEWARAT P, et al. Feeding-regimen of β-glucan to enhance innate immunity and disease resistance of Nile tilapia, Oreochromis niloticus Linn, against Aeromonas hydrophila and Flavobacterium columnare[J]. Fish Shellfish Immunol, 2019, 87: 120-128. doi: 10.1016/j.fsi.2018.12.062
    [31]
    JONES S E, LENNON J T. Dormancy contributes to the maintenance of microbial diversity[J]. P Natl Acad Sci USA, 2010, 107(13): 5881-5886. doi: 10.1073/pnas.0912765107
    [32]
    WU J F, XIONG J B, WANG X, et al. Intestinal bacterial community is indicative for the healthy status of Litopenaeus vannamei[J]. Chin J Appl Ecol, 2016, 27(2): 611-621.
    [33]
    韩少锋. 罗非鱼腐败过程菌群结构分析及腐败菌的分离、鉴定与调控[D]. 北京: 中国农业科学院, 2010: 7-33.
    [34]
    MUKHOPADHYA I, HANSEN R, ELOMAR E M, et al. IBD: what role do proteobacteria play?[J]. Nat Rev Gastro Hepat, 2012, 9(4): 219-230. doi: 10.1038/nrgastro.2012.14
    [35]
    于明超, 张晓华, 郑艳芬, 等. 一种运动鲁杰氏菌菌株及其应用: CN105779366B[P]. 2019-05-31.
    [36]
    KO S R, JEONG Y, CHO S H, et al. Functional role of a novel algicidal compound produced by Pseudoruegeria sp. M32A2M on the harmful algae Alexandrium catenella[J]. Chemosphere, 2022, 300: 1-9.
    [37]
    陈晓瑛, 王国霞, 孙育平, 等. 饲料中添加低聚木糖对凡纳滨对虾幼虾消化酶活力、肠道形态及细菌数量的影响[J]. 动物营养学报, 2018, 30(4): 1522-1529.
    [38]
    SU P, HAN Y, JIANG C, et al. Effects of chitosan ligosaccharides on growth performance, digestive enzyme and intestinal bacterial flora of tiger puffer (Takifugu rubripes Temminck et Schlegel, 1850)[J]. J Appl Ichthyol, 2017, 33(3): 458-467. doi: 10.1111/jai.13282
    [39]
    潘金露. 饲料中壳寡糖和褐藻酸寡糖对大菱鲆(Scophthalmus maximus)消化及肠道菌群的影响[D]. 大连: 大连海洋大学, 2016: 19-24.
    [40]
    殷朝敏, 高虹, 范秀芝, 等. 一种增强白参菌多糖生物活性的复合改性方法及其在调节人体肠道菌群中的应用: CN202210822554.4[P]. 2022-07-13.
    [41]
    曹海鹏, 温乐夫, 周桂娴, 等. 南美白对虾白便综合征病原霍乱弧菌的分离与药敏试验[J]. 动物医学进展, 2016, 37(2): 128-132.
    [42]
    PESTOVA M I, CLIFT R E, VICKERS R J, et al. Effect of weaning and dietary galactose supplementation on digesta glycoproteins in pigs[J]. J Sci Food Agric, 2000, 80(13): 1918-1924. doi: 10.1002/1097-0010(200010)80:13<1918::AID-JSFA731>3.0.CO;2-B
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