SONG Xue, LIU Han, LI Zhaoxuan, LI Bafang, HOU Hu. Preparation and properties of medical compound hemostasis dressing from Pacific cod skin[J]. South China Fisheries Science, 2022, 18(2): 66-73. DOI: 10.12131/20210323
Citation: SONG Xue, LIU Han, LI Zhaoxuan, LI Bafang, HOU Hu. Preparation and properties of medical compound hemostasis dressing from Pacific cod skin[J]. South China Fisheries Science, 2022, 18(2): 66-73. DOI: 10.12131/20210323

Preparation and properties of medical compound hemostasis dressing from Pacific cod skin

More Information
  • Received Date: October 31, 2021
  • Revised Date: December 12, 2021
  • Accepted Date: January 09, 2022
  • Available Online: January 26, 2022
  • In this study, an absorbable composite hemostatic dressing was prepared by combining sodium alginate with Pacific cod (Gadus macrocephalus) skin gelatin, and its mechanical properties, hemostatic effect, hemostatic mechanism and biocompatibility were evaluated. The results show that the composite hemostatic dressing had high mechanical strength with 1% of gelatin concentration. Results of hemostatic evaluation in vivo indicate that the dressing had good hemostatic effect on the femoral artery, liver wound and tail hemostasis models of rats, and the hemostatic time was (64±9), (108±4) and (230±25) s, respectively, which was similar with the hemostatic effect of commercial gelatin sponge. The dressing could shorten activated partial thromboplastin time (APTT) and thrombin time (TT), which activated endogenous coagulation and common coagulation pathway, and promoted the release of thromboxane-B2 (TXB2), platelet factor-4 (PF4) and P-selectin to achieve rapid hemostatic effect. Biocompatibility experiments show that the composite dressing had no acute toxicity or irritation and the hemolysis rate was less than 5%, which meets the standards of medical materials. The study provides reference and theoretical basis for developing new medical dressing and realizing high-value utilization of Pacific cod skin.
  • [1]
    BURNETT L R, RICHTER J G, RAHMANY M B, et al. Novel keratin (KeraStat™) and polyurethane (Nanosan®-Sorb) biomaterials are hemostatic in a porcine lethal extremity hemorrhage model[J]. J Biomater Appl, 2014, 28(6): 869-879. doi: 10.1177/0885328213484975
    [2]
    LAN G, LU B, WANG T, et al. Chitosan/gelatin composite sponge is an absorbable surgical hemostatic agent[J]. Colloids Surf B, 2015, 136: 1026-1034. doi: 10.1016/j.colsurfb.2015.10.039
    [3]
    FELGUEIRAS H P, AMORIM M T P. Functionalization of electrospun polymeric wound sponges with antimicrobial peptides[J]. Colloids Surf B, 2017, 156: 133-148. doi: 10.1016/j.colsurfb.2017.05.001
    [4]
    陈胜军, 李来好, 杨贤庆, 等. 罗非鱼综合加工利用与质量安全控制技术研究进展[J]. 南方水产科学, 2011, 7(4): 85-90. doi: 10.3969/j.issn.2095-0780.2011.04.013
    [5]
    蓝广芊. 壳聚糖/明胶复合止血材料的研制及其性能研究[D]. 重庆: 西南大学, 2016, 5-8.
    [6]
    SADOWSKA M, KOLADZIEJSKA I, NIECIKOWSKA C. Isolation of collagen from the skins of Baltic cod (Gadus morhua)[J]. Food Chem, 2003, 81(2): 257-262. doi: 10.1016/S0308-8146(02)00420-X
    [7]
    LI D, YE Y, LI D, et al. Biological properties of dialdehyde carboxymethyl cellulose crosslinked gelatin-peg composite hydrogel fibers for wound dressings[J]. Carbohydr Polym, 2015, 137: 508-514.
    [8]
    BARHAM A S, TEWES F, HEALY A M. Moisture diffusion and permeability characteristics of hydroxypropyl methylcellulose and hard gelatin capsules[J]. Int J Pharm, 2015, 478(2): 796-803. doi: 10.1016/j.ijpharm.2014.12.029
    [9]
    CHEN H, XING X, TAN H, et al. Covalently antibacterial alginate-chitosan hydrogel dressing integrated gelatin microspheres containing tetracycline hydrochloride for wound healing[J]. Mater Sci Eng C, 2017, 70: 287-295. doi: 10.1016/j.msec.2016.08.086
    [10]
    WANG K, NUNE K C, MISRA R D K. The functional response of alginate-gelatin-nanocrystalline cellulose injectable hydrogels toward delivery of cells and bioactive molecules[J]. Acta Biomater, 2016, 36: 143-151. doi: 10.1016/j.actbio.2016.03.016
    [11]
    LIU S, LI Y, LI L. Enhanced stability and mechanical strength of sodium alginate composite films[J]. Carbohydr Polym, 2017, 160: 62-70. doi: 10.1016/j.carbpol.2016.12.048
    [12]
    谭国忠, 涂欣冉, 郭黎洋, 等. 3D打印明胶/海藻酸钠/58S生物玻璃骨缺损修复支架的生物安全性评价[J]. 中国组织工程研究, 2022, 26(4): 545-551.
    [13]
    孙士儒, 刘阳, 王景辉, 等. 具有不同拓扑结构的海藻酸钠-明胶复合水凝胶的3D打印制备及其性能[J]. 复合材料学报, 2021, 39. DOI: 10.13801/j.cnki.fhclxb.20210917.001.
    [14]
    HOU H, LI B, ZHAO X, et al. The effect of Pacific cod (Gadus macrocephalus) skin gelatin polypeptides on UV radiation-induced skin photoaging in ICR mice[J]. Food Chem, 2009, 115(3): 945-950. doi: 10.1016/j.foodchem.2009.01.015
    [15]
    LAEMMLI U K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4[J]. Nature, 1970, 227(5259): 680. doi: 10.1038/227680a0
    [16]
    YAN J, MIAO Y, TAN H, et al. Injectable alginate/hydroxyapatite gel scaffold combined with gelatin microspheres for drug delivery and bone tissue engineering[J]. Mater Sci Eng C, 2016, 63: 274-284. doi: 10.1016/j.msec.2016.02.071
    [17]
    HAN F, DONG Y, SU Z, et al. Preparation, characteristics and assessment of a novel gelatin-chitosan sponge scaffold as skin tissue engineering material[J]. Int J Pharm, 2014, 476(1/2): 124-133.
    [18]
    SAFANDOWSKA M, PIETRUCHA K. Effect of fish collagen modification on its thermal and rheological properties[J]. Int J Biol Macromol, 2013, 53: 32-37. doi: 10.1016/j.ijbiomac.2012.10.026
    [19]
    DHAND C, VENKATESH M, BARATHI V A, et al. Bio-inspired crosslinking and matrix-drug interactions for advanced wound dressings with long-term antimicrobial activity[J]. Biomaterials, 2017: 153-168.
    [20]
    DUAN R, ZHANG J, LIU, L, et al. The functional properties and application of gelatin derived from the skin of channel catfish (Ictalurus punctatus)[J]. Food Chem, 2018, 239(15): 464-469.
    [21]
    SEE S F, GHASSEM M, MAMOT S, et al. Effect of different pretreatments on functional properties of African catfish (Clarias gariepinus) skin gelatin[J]. J Food Sci Tech, 2015, 52(2): 753-762. doi: 10.1007/s13197-013-1043-6
    [22]
    王运智. 两种鱼皮胶原止血海绵理化性能研究与生物学评价[D]. 烟台: 烟台大学, 2019: 16-17.
    [23]
    KOOSEHGOL S, EBRAHIMIAN-HOSSEINABADI M, ALIZADEH M, et al. Preparation and characterization of in situ chitosan/polyethylene glycol fumarate/thymol hydrogel as an effective wound dressing[J]. Mater Sci Eng C, 2017, 79: 66-75. doi: 10.1016/j.msec.2017.05.001
    [24]
    WANG C, LUO W, LI P, et al. Preparation and evaluation of chitosan/alginate porous microspheres/Bletilla striata, polysaccharide composite hemostatic sponges[J]. Carbohydr Polym, 2017, 174: 432-442.
    [25]
    MIRZAKHANIAN Z, FAGHIHI K, BARATI A, et al. Synthesis and characterization of fast-swelling porous superabsorbent hydrogel based on starch as a hemostatic agent[J]. J Biomater Sci Polym Ed, 2015, 26(18): 1-13.
    [26]
    LI L, DU Y, YIN Z, et al. Preparation and the hemostatic property study of porous gelatin microspheres both in vitro and in vivo[J]. Colloids Surf B, 2019, 187: 110641.
    [27]
    ZHANG K, LI J, WANG Y, et al. Hydroxybutyl chitosan/diatom-biosilica composite sponge for hemorrhage control[J]. Carbohydr Polym, 2020, 236: 116051. doi: 10.1016/j.carbpol.2020.116051
    [28]
    LI H, CHENG W, LIU K, et al. Reinforced collagen with oxidized microcrystalline cellulose shows improved hemostatic effects[J]. Carbohydr Polym, 2017, 165: 30-38. doi: 10.1016/j.carbpol.2017.02.023
    [29]
    LIU Y, LIU Y, LIAO N, et al. Fabrication and durable antibacterial properties of electrospun chitosan nanofibers with silver nanoparticles[J]. Int J Biol Macromol, 2015, 79: 638-643. doi: 10.1016/j.ijbiomac.2015.05.058
    [30]
    SARIKA P R, JAMES N R. Polyelectrolyte complex nanoparticles from cationised gelatin and sodium alginate for curcumin delivery[J]. Carbohydr Polym, 2016, 148: 354-361. doi: 10.1016/j.carbpol.2016.04.073

Catalog

    Recommendations
    Application of fishery acoustic frequency difference technology in fishery resource assessment of marine ranching in southern sea area of yintan, guangxi
    CHEN Guobao et al., SOUTH CHINA FISHERIES SCIENCE, 2025
    Trophic niches ofceratoscopelus warmingiiandbolinichthys longipesin adjacent waters of cold seep area in south china sea
    SHI Juan et al., SOUTH CHINA FISHERIES SCIENCE, 2024
    Estimation on catches by guangxi fishing vessels in beibu gulf
    GONG Binhao et al., SOUTH CHINA FISHERIES SCIENCE, 2024
    Assessment of fishery resources in southern sea area of yintan marine ranching, guangxi province
    NIU Lulian et al., SOUTH CHINA FISHERIES SCIENCE, 2024
    Study on community structure and seasonal dynamics of zooplankton and macrobenthos in rice-fish symbiotic system in qingtian, zhejiang province
    ZHENG Sidi et al., JOURNAL OF FISHERIES OF CHINA, 2025
    Difference in the fishery resource density between the bottom and surface layers and an analysis of multiple types of related factor importance in the northern south china sea
    SUN Mingshuai et al., JOURNAL OF FISHERY SCIENCES OF CHINA, 2024
    The diversity and community composition of three plants' rhizosphere fungi in kaolin mining areas
    Xiao, Wenqi et al., JOURNAL OF FUNGI, 2024
    Spatio-temporal distribution of harmful algal blooms and their correlations with marine hydrological elements in offshore areas, china
    Chen, Chao et al., OCEAN & COASTAL MANAGEMENT, 2023
    Morphological and phylogenetical analyses of pathogenic hypomyces perniciosus isolates from agaricus bisporus causing wet bubble disease in china
    PHYTOTAXA, 2021
    Exploring water quality as a determinant of the existence of soft shell crab (scylla olivacea) in different habitats of the large marine ecosystem in the bay of bengal.
    WATER CONSERVATION AND MANAGEMENT, 2024
    Powered by
    Article views PDF downloads Cited by()
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return