Preparation and properties of medical compound hemostasis dressing from Pacific cod skin

In this study, an absorbable composite hemostatic dressing was prepared by combining sodium alginate with Pacific cod (Gadus macrocephalus) skin gelatin, and its mechanical properties, hemostatic effect, hemostatic mechanism and biocompatibility were evaluated. The results show that the composite hemostatic dressing had high mechanical strength with 1% of gelatin concentration. Results of hemostatic evaluation in vivo indicate that the dressing had good hemostatic effect on the femoral artery, liver wound and tail hemostasis models of rats, and the hemostatic time was (64±9), (108±4) and (230±25) s, respectively, which was similar with the hemostatic effect of commercial gelatin sponge. The dressing could shorten activated partial thromboplastin time (APTT) and thrombin time (TT), which activated endogenous coagulation and common coagulation pathway, and promoted the release of thromboxane-B₂ (TXB₂), platelet factor-4 (PF4) and P-selectin to achieve rapid hemostatic effect. Biocompatibility experiments show that the composite dressing had no acute toxicity or irritation and the hemolysis rate was less than 5%, which meets the standards of medical materials. The study provides reference and theoretical basis for developing new medical dressing and realizing high-value utilization of Pacific cod skin.