Volume 18 Issue 3
Jun.  2022
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SUN Yuanchen, XU Bingjie, CAO Yichou, XU Yingjie, QIN Fenju, YUAN Hongxia. Protective effects of addition of nano cerium oxide in diets on Eriocheir sinensis under ammonia-nitrogen and Aeromonas hydrophila stresses[J]. South China Fisheries Science, 2022, 18(3): 94-101. DOI: 10.12131/20210209
Citation: SUN Yuanchen, XU Bingjie, CAO Yichou, XU Yingjie, QIN Fenju, YUAN Hongxia. Protective effects of addition of nano cerium oxide in diets on Eriocheir sinensis under ammonia-nitrogen and Aeromonas hydrophila stresses[J]. South China Fisheries Science, 2022, 18(3): 94-101. DOI: 10.12131/20210209
shu

Protective effects of addition of nano cerium oxide in diets on Eriocheir sinensis under ammonia-nitrogen and Aeromonas hydrophila stresses

  • School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou 215009, China

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  • Received Date: July 25, 2021
  • Revised Date: October 22, 2021
  • Accepted Date: November 04, 2021
  • Available Online: November 09, 2021
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    • Abstract
  • To explore the mechanism of Chinese mitten crabs (Eriocheir sinensis) dealing with adverse water environmental stresses, we studied the effects of nano cerium oxide (nCeO2) on the immunity and antioxidant capacity of the crabs under ammonia-nitrogen and Aeromonas hydrophila stresses. The crabs were continuously fed with diets supplemented with different doses of nCeO2 (0, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 and 12.8 mg·kg−1) for 90 d, then the ammonia-nitrogen and A. hydrophila stress tests were conducted. The results show that: 1) Both the single ammonia-nitrogen stress and dual stresses of ammonia-nitrogen and A. hydrophila (henceforth called "dual stress") decreased the survival rates of the crabs significantly (P<0.01); the activities of acid phosphatase (ACP), alkaline phosphatase (AKP) and lysosome (LZM) increased (P<0.01 or P<0.05); the activities of superoxide dismutase (SOD) and catalase (CAT) decreased significantly (P<0.01), while the concentration of malondialdehyde (MDA) increased (P<0.01 or P<0.05); 2) Suitable nCeO2 dose could enhance the survival rates of crabs under the single ammonia-nitrogen stress and dual stress (P<0.01 or P<0.05), increase the activities of SOD and CAT significantly (P<0.01), decrease the concentration of MDA (P<0.01 or P<0.05), further increase the activity of LZM (P<0.01), but the activities of ACP and AKP only increased under dual stress (P<0.01). Above all, suitable supplementation of nCeO2 can ameliorate the decline of survival rates and antioxidant capacity of E. sinensis under the the single ammonia-nitrogen stress and dual stress, and increase the immunity enzyme activities. The suitable nCeO2 supplementation doses for protecting crabs are 0.8 and 1.6 mg·kg−1 under single and dual stress, respectively.
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