Volume 18 Issue 3
Jun.  2022
Turn off MathJax
Article Contents
Abstract
References
Related Articles
SUN Yuanchen, XU Bingjie, CAO Yichou, XU Yingjie, QIN Fenju, YUAN Hongxia. Protective effects of addition of nano cerium oxide in diets on Eriocheir sinensis under ammonia-nitrogen and Aeromonas hydrophila stresses[J]. South China Fisheries Science, 2022, 18(3): 94-101. DOI: 10.12131/20210209
Citation: SUN Yuanchen, XU Bingjie, CAO Yichou, XU Yingjie, QIN Fenju, YUAN Hongxia. Protective effects of addition of nano cerium oxide in diets on Eriocheir sinensis under ammonia-nitrogen and Aeromonas hydrophila stresses[J]. South China Fisheries Science, 2022, 18(3): 94-101. DOI: 10.12131/20210209
shu

Protective effects of addition of nano cerium oxide in diets on Eriocheir sinensis under ammonia-nitrogen and Aeromonas hydrophila stresses

  • School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou 215009, China

More Information
  • Received Date: July 25, 2021
  • Revised Date: October 22, 2021
  • Accepted Date: November 04, 2021
  • Available Online: November 09, 2021
    Graphical Abstract
    • Abstract
  • To explore the mechanism of Chinese mitten crabs (Eriocheir sinensis) dealing with adverse water environmental stresses, we studied the effects of nano cerium oxide (nCeO2) on the immunity and antioxidant capacity of the crabs under ammonia-nitrogen and Aeromonas hydrophila stresses. The crabs were continuously fed with diets supplemented with different doses of nCeO2 (0, 0.2, 0.4, 0.8, 1.6, 3.2, 6.4 and 12.8 mg·kg−1) for 90 d, then the ammonia-nitrogen and A. hydrophila stress tests were conducted. The results show that: 1) Both the single ammonia-nitrogen stress and dual stresses of ammonia-nitrogen and A. hydrophila (henceforth called "dual stress") decreased the survival rates of the crabs significantly (P<0.01); the activities of acid phosphatase (ACP), alkaline phosphatase (AKP) and lysosome (LZM) increased (P<0.01 or P<0.05); the activities of superoxide dismutase (SOD) and catalase (CAT) decreased significantly (P<0.01), while the concentration of malondialdehyde (MDA) increased (P<0.01 or P<0.05); 2) Suitable nCeO2 dose could enhance the survival rates of crabs under the single ammonia-nitrogen stress and dual stress (P<0.01 or P<0.05), increase the activities of SOD and CAT significantly (P<0.01), decrease the concentration of MDA (P<0.01 or P<0.05), further increase the activity of LZM (P<0.01), but the activities of ACP and AKP only increased under dual stress (P<0.01). Above all, suitable supplementation of nCeO2 can ameliorate the decline of survival rates and antioxidant capacity of E. sinensis under the the single ammonia-nitrogen stress and dual stress, and increase the immunity enzyme activities. The suitable nCeO2 supplementation doses for protecting crabs are 0.8 and 1.6 mg·kg−1 under single and dual stress, respectively.
    • FullText(HTML)
    • References (41)
  • [1]
    农业部渔业渔政管理局. 2020中国渔业统计年鉴[M]. 北京: 中国农业出版社, 2020: 24.
    [2]
    PAN L, SI L, LIU S, et al. Levels of metabolic enzymes and nitrogenous compounds in the swimming crab Portunus trituberculatus exposed to elevated ambient ammonia-N[J]. J Ocean U China, 2018, 17(4): 957-966. doi: 10.1007/s11802-018-3574-y
    [3]
    SEO J S, HAQUE M N, NAM S E, et al. Inorganic nitrogen compounds reduce immunity and induce oxidative stress in red seabream[J]. Fish Shellfish Immunol, 2020, 104: 237-244. doi: 10.1016/j.fsi.2020.05.072
    [4]
    王安琪, 郭立, 崔培, 等. 不同水平低聚木糖对凡纳滨对虾生长、免疫及抗氨氮胁迫能力的影响[J]. 中国饲料, 2019(7): 56-65.
    [5]
    彭军辉, 陈丽英, 程长洪, 等. 氨氮对拟穴青蟹的急性毒性及对其血清免疫相关酶活力的影响[J]. 渔业科学进展, 2018, 39(5): 114-121.
    [6]
    曾媛媛, 蒋云霞, 艾春香. 氨氮胁迫对拟穴青蟹组织器官中SOD及GPX活性的影响[J]. 台湾海峡, 2011, 30(2): 210-215.
    [7]
    成亚辉, 王子艺, 高栋业, 等. 噬菌体防治嗜水气单胞菌相关疾病的研究进展[J]. 贵州农业科学, 2020, 48(11): 48-53. doi: 10.3969/j.issn.1001-3601.2020.11.012
    [8]
    程超, 肖敏, 李菁, 等. 嗜水气单胞菌刺激对中华绒螯蟹免疫的影响[J]. 水产科学, 2020, 39(4): 465-475.
    [9]
    张磊. 中华鳖养殖水体的环境因子调查与机体致病菌研究[D]. 保定: 河北大学, 2009: 18-40.
    [10]
    岳峰, 潘鲁青, 谢鹏, 等. 氨氮胁迫对三疣梭子蟹酚氧化酶原系统和免疫指标的影响[J]. 中国水产科学, 2010, 17(4): 761-770.
    [11]
    SARAVANAKUMAR K, SATHIYASEELAN A, MARIADOSS A V A, et al. Antioxidant and antidiabetic properties of biocompatible ceria oxide (CeO2) nanoparticles in mouse fibroblast NIH3T3 and insulin resistant HepG2 cells[J]. Ceram Int, 2021, 47(6): 8618-8626. doi: 10.1016/j.ceramint.2020.11.230
    [12]
    AMIRI F T, HAMZEH M, BEKLAR S Y, et al. Anti-apoptotic and antioxidant effect of cerium oxide nanoparticles on cyclophosphamide-induced hepatotoxicity[J]. Erciyes Med J, 2018, 40(3): 148-54. doi: 10.5152/etd.2018.0016
    [13]
    ADEBAYO O A, AKINLOYE O, ADARAMOYE O A. Cerium oxide nanoparticles attenuate oxidative stress and inflammation in the liver of diethylnitrosamine-treated mice[J]. Biol Trace Elem Res, 2020, 193(1): 214-225. doi: 10.1007/s12011-019-01696-5
    [14]
    QIN F, SHEN T, YANG H, et al. Dietary nano cerium oxide promotes growth, relieves ammonia nitrogen stress, and improves immunity in crab (Eriocheir sinensis)[J]. Fish Shellfish Immun, 2019, 92: 367-376. doi: 10.1016/j.fsi.2019.06.019
    [15]
    ZHAO J H, LAM T J, GUO J Y. Acute toxicity of ammonia to the early stage ‐ larvae and juveniles of Eriocheir sinensis H. Milne-Edwards, 1853 (Decapoda: Grapsidae) reared in the laboratory[J]. Aquac Res, 1997, 28(7): 517-525. doi: 10.1111/j.1365-2109.1997.tb01070.x
    [16]
    CONG M, WU H, CAO T, et al. Digital gene expression analysis in the gills of Ruditapes philippinarum exposed to short-and long-term exposures of ammonia nitrogen[J]. Aquat Toxicol, 2018, 194: 121-131. doi: 10.1016/j.aquatox.2017.11.012
    [17]
    FRÍAS-ESPERICUETA M G, HARFUSH-MELENDEZ M, OSUNA-LÓPEZ J I, et al. Acute toxicity of ammonia to juvenile shrimp Penaeus vannamei Boone[J]. Bull Environ Contam Toxicol, 1999, 62(5): 646-652. doi: 10.1007/s001289900923
    [18]
    CHENG C H, YANG F F, LIAO S A, et al. Effect of acute ammonia exposure on expression of GH/IGF axis genes GHR1, GHR2 and IGF-1 in pufferfish (Takifugu obscurus)[J]. Fish Physiol Biochem, 2015, 41(2): 495-507. doi: 10.1007/s10695-015-0025-1
    [19]
    黄鹤忠, 李义, 宋学宏, 等. 氨氮胁迫对中华绒螯蟹 (Eriocheir sinensis) 免疫功能的影响[J]. 海洋与湖沼, 2006, 37(3): 198-205. doi: 10.3321/j.issn:0029-814X.2006.03.002
    [20]
    SHI M, JIANG S, LI Y, et al. Comprehensive expression analysis of the beta integrin from Penaeus monodon indicating its participation in innate immunity and ammonia nitrogen stress response[J]. Fish Shellfish Immunol, 2020, 98: 887-898. doi: 10.1016/j.fsi.2019.11.049
    [21]
    HIRST S M, KARAKOTO A, SINGH S, et al. Bio-distribution and in vivo antioxidant effects of cerium oxide nanoparticles in mice[J]. Environ Toxicol, 2013, 28(2): 107-118. doi: 10.1002/tox.20704
    [22]
    AMIN K A, HASSAN M S, AWAD E S T, et al. The protective effects of cerium oxide nanoparticles against hepatic oxidative damage induced by monocrotaline[J]. Int J Nanomedicine, 2011, 6: 143. doi: 10.2217/nnm.10.139
    [23]
    CHECA J, ARAN J M. Reactive oxygen species: drivers of physiological and pathological processes[J]. J Inflamm Res, 2020, 13: 1057-1073. doi: 10.2147/JIR.S275595
    [24]
    ABRAMOV A Y, POTAPOVA E V, DREMIN V V, et al. Interaction of oxidative stress and misfolded proteins in the mechanism of neurodegeneration[J]. Life (Basel), 2020, 10(7): 101.
    [25]
    BARBOSA M L, de Meneses A A P M, de AGUIAR R P S, et al. Oxidative stress, antioxidant defense and depressive disorders: a systematic review of biochemical and molecular markers[J]. Neurol Psychiatry Brain Res, 2020, 36: 65-72. doi: 10.1016/j.npbr.2020.02.006
    [26]
    JING H, ZHANG Q, GAO X J. Excessive lithium of water induced a toxic effect on kidney via oxidative damage and inflammation in carp[J]. Aquaculture, 2020, 535(5): 736282.
    [27]
    DONG J, CHENG R, YANG Y, et al. Effects of dietary taurine on growth, non-specific immunity, anti-oxidative properties and gut immunity in the Chinese mitten crab Eriocheir sinensis[J]. Fish Shellfish Immunol, 2018, 82: 212-219. doi: 10.1016/j.fsi.2018.08.029
    [28]
    YANG X, SHI A, SONG Y, et al. The effects of ammonia-N stress on immune parameters, antioxidant capacity, digestive function, and intestinal microflora of Chinese mitten crab, Eriocheir sinensis, and the protective effect of dietary supplement of melatonin[J]. Comp Biochem Phys C, 2021, 250: 109127.
    [29]
    SINHA A K, ABDEIGAWAD H, GIBLEN T, et al. Anti-oxidative defences are modulated differentially in three freshwater teleosts in response to ammonia-induced oxidative stress[J]. PLOS ONE, 2014, 9(4): e95319. doi: 10.1371/journal.pone.0095319
    [30]
    管敏, 张德志, 唐大明. 慢性氨氮胁迫对史氏鲟幼鱼生长及其肝脏抗氧化, 免疫指标的影响[J]. 南方水产科学, 2020, 16(2): 36-42. doi: 10.12131/20190191
    [31]
    洪美玲, 陈立侨, 顾顺樟, 等. 氨氮胁迫对中华绒螯蟹免疫指标及肝胰腺组织结构的影响[J]. 中国水产科学, 2007, 14(3): 412-418. doi: 10.3321/j.issn:1005-8737.2007.03.010
    [32]
    WEI J, YU N, TIAN W, et al. Dietary vitamin B12 requirement and its effect on non-specific immunity and disease resistance in juvenile Chinese mitten crab Eriocheir sinensis[J]. Aquaculture, 2014, 434: 179-183. doi: 10.1016/j.aquaculture.2014.08.010
    [33]
    LIU F, QU Y K, GENG C, et al. Effects of hesperidin on the growth performance, antioxidant capacity, immune responses and disease resistance of red swamp crayfish (Procambarus clarkii)[J]. Fish Shellfish Immunol, 2020, 99: 154-166. doi: 10.1016/j.fsi.2020.02.014
    [34]
    XUE Q, RENAULT T. Enzymatic activities in European flat oyster, Ostrea edulis, and Pacific oyster, Crassostrea gigas, hemolymph[J]. J Invertebr Pathol, 2000, 76(3): 155-163. doi: 10.1006/jipa.2000.4965
    [35]
    SAURABH S, SAHOO P K. Lysozyme: an important defence molecule of fish innate immune system[J]. Aquac Res, 2008, 39(3): 223-239. doi: 10.1111/j.1365-2109.2007.01883.x
    [36]
    RAGLAND S A, CRISS A K. From bacterial killing to immune modulation: recent insights into the functions of lysozyme[J]. PLOS Pathog, 2017, 13(9): e1006512. doi: 10.1371/journal.ppat.1006512
    [37]
    MOCK A, PETERS G. Lysozyme activity in rainbow trout, Oncorhynchus mykiss (Walbaum), stressed by handling, transport and water pollution[J]. J Fish Biol, 1990, 37(6): 873-885. doi: 10.1111/j.1095-8649.1990.tb03591.x
    [38]
    KUEBUTORNYE F K A, WANG Z, LU Y, et al. Effects of three host-associated Bacillus species on mucosal immunity and gut health of Nile tilapia, Oreochromis niloticus and its resistance against Aeromonas hydrophila infection[J]. Fish Shellfish Immunol, 2020, 97: 83-95. doi: 10.1016/j.fsi.2019.12.046
    [39]
    艾春香, 曾媛媛. 氨氮胁迫对拟穴青蟹腺苷三磷酸酶和磷酸酶比活力的影响[J]. 厦门大学学报 (自然科学版), 2011, 50(4): 772-778.
    [40]
    REDLING K. Rare earth elements in agriculture with emphasis on animal husbandry[D]. Muenchen: Diss Ludwig-Maximilians-Universitaet, 2006: 325.
    [41]
    PAEMEL M V, DIERICK N, JANSSENS G, et al. Selected trace and ultratrace elements: biological role, content in feed and requirements in animal nutrition-elements for risk assessment[J]. EFSA Supporting Publications, 2010, 7(7): 68E.
    • Related Articles

  • Related Articles

    [1]XU Bo, YUAN Hongchun. Research on fish feeding intensity classification model based on axial feature calibration and temporal segment network[J]. South China Fisheries Science, 2024, 20(6): 145-154. DOI: 10.12131/20240200
    [2]QIAN Cheng, ZHANG Jiapeng, TU Xueying, LIU Huang, QIAO Gan, LIU Shijing. Turbot fish egg recognition and counting method based on CBAM-UNet[J]. South China Fisheries Science, 2024, 20(6): 132-144. DOI: 10.12131/20240123
    [3]ZHANG Hongjiao, ZHANG Cunxi, WANG Rui, WANG Ke, QIAO Qian. Freshness recognition of small yellow croaker based on image processing and improved DenseNet network[J]. South China Fisheries Science, 2024, 20(3): 133-142. DOI: 10.12131/20230241
    [4]GONG Yuanjin, YANG Shujie. Dynamic water surface environment perception SLAM algorithm based on visual semantics and point-line fusion for unmanned surface vessels[J]. South China Fisheries Science, 2024, 20(3): 120-132. DOI: 10.12131/20240019
    [5]YUAN Yongming, SHI Pei. Study on fish movement monitoring method based on image processing[J]. South China Fisheries Science, 2018, 14(5): 109-114. DOI: 10.3969/j.issn.2095-0780.2018.05.014
    [6]LI Laihao, SUN Bolun, ZHAO Donghao. Research progress in detection and preparation methods for tetrodotoxin[J]. South China Fisheries Science, 2018, 14(3): 126-132. DOI: 10.3969/j.issn.2095-0780.2018.03.016
    [7]TANG Xianming, WANG Yingrui, LIU Cui, CHEN Fuxiao, YIN Yi, LIU Tao. Prelimary study of segment culture of Kappaphycus alvarezii[J]. South China Fisheries Science, 2014, 10(2): 36-41. DOI: 10.3969/j.issn.2095-0780.2014.02.005
    [8]HUANG Chunli, HUANG He, LIU Wenxia, GAO Ping, HUANG Guofang, LI Zhiqing, CHENG Hong, LUO Lin. Research progress on residual toxicity and detection methods of melamine[J]. South China Fisheries Science, 2011, 7(3): 76-80. DOI: 10.3969/j.issn.2095-0780.2011.03.014
    [9]ZHAO Fei, ZOU Weimin. Application of LAMP in the rapid detection of aquatic animal pathogens[J]. South China Fisheries Science, 2007, 3(2): 71-75.
    [10]WANG Li, LIN Hong, CAO Limin. Detection of aminoglycosides in foods of animal origin[J]. South China Fisheries Science, 2006, 2(1): 76-79.

  • Cited by

    Periodical cited type(6)

    1. 徐菲，徐开达，张洪亮，卢占晖，周永东，李羽如，叶莹莹，马文静，金梓慧. 浙江岱衢洋海域春秋季游泳动物群落结构及生物量粒径谱特征. 海洋与湖沼. 2025(01): 165-174 .
    2. Zhisen LUO，Murong YI，Xiaodong YANG，Xiao CHEN，Jinxi WANG，Changping JIANG，Fengming LIU，Konglan LUO，Xiongbo HE，Hung-Du LIN，Bin KANG，Yunrong YAN. Checklist of marine fishes in the Beibu Gulf: fish classification, resource protection, and biodiversity challenge. Journal of Oceanology and Limnology. 2025(01): 232-247 .
    3. 李诗佳，施利燕，钟俊生，赵路路. 长江口南部水域春、秋季鱼类群落结构比较. 上海海洋大学学报. 2024(01): 135-149 .
    4. 李淼，许友伟，孙铭帅，范江涛，李佳俊，张魁，陈作志. 拉尼娜事件前后北部湾鱼类群落结构变化研究. 南方水产科学. 2023(02): 1-11 . 本站查看
    5. Chenyu Song，Zhong Tu，Na Song. Discordant patterns of genetic variation between mitochondrial and microsatellite markers in Acanthogobius ommaturus across the coastal areas of China. Acta Oceanologica Sinica. 2023(04): 72-80 .
    6. 王鑫，李昌文，徐加涛，李士虎，宋可心，唐佳伟，马晓娜，冯志华. 灌河口海域水生动物群落结构及分布特征. 江苏海洋大学学报(自然科学版). 2023(04): 29-41 .

    Other cited types(7)

Catalog

    Get Citation
    PDF
    XML
    Article views (626) PDF downloads (66) Cited by(13)
    Related

    Supported by: Beijing Renhe Information Technology Co., Ltd.  

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return