YANG Xiaotong, YE Lingtong, LU Jie, YAO Tuo, WANG Jiangyong, FU Yaowu, ZHANG Qizhong. Epidemiology investigation of Perkinsus spp. in shellfish along coastal area of Guangdong Province[J]. South China Fisheries Science, 2022, 18(1): 128-134. DOI: 10.12131/20210183
Citation: YANG Xiaotong, YE Lingtong, LU Jie, YAO Tuo, WANG Jiangyong, FU Yaowu, ZHANG Qizhong. Epidemiology investigation of Perkinsus spp. in shellfish along coastal area of Guangdong Province[J]. South China Fisheries Science, 2022, 18(1): 128-134. DOI: 10.12131/20210183

Epidemiology investigation of Perkinsus spp. in shellfish along coastal area of Guangdong Province

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  • Received Date: June 23, 2021
  • Revised Date: July 29, 2021
  • Accepted Date: September 02, 2021
  • Available Online: September 18, 2021
  • Shellfish are generally cultured in open sea areas, which makes it difficult to carry out shellfish disease prevention and control. Therefore, investigating the epidemic infection law of pathogens is particularly important for the occurrence, development and control of shellfish pathogens. In this paper, we applied common PCR (Polymerase Chain Reaction) technology to investigate the seasonal dynamics and spatio-temporal distribution of Perkinsus spp. among five mainly cultivated shellfish in the six aquaculture locations along the coastal area of Guangdong Province. Two Perkinsus spp. were detected (P. beihaiensis and P. olseni). The prevalence of P. beihaiensis was significantly higher than that of P. olseni. Among the five sampled shellfish species except Crassostrea angulate, all were infected by Perkinsus spp., and C. hongkongensis and Haliotis diversicolor were infected by two Perkinsus spp., while Pinctada martensii and Chlamys nobilis were only infected by P. olseni. The prevalence of Perkinsus spp. in western Guangdong, including Zhanjiang, Yangjiang and Jiangmen was 17.17%, significantly higher than that in eastern Guangdong, including Shanwei, Jieyang and Shantou. The prevalence of Perkinsus spp. in summer and autumn was 15.67%, significantly higher than that in winter and spring. In different coastal areas, the seasonal occurrence variation of Perkinsus spp. might be affected by the host's culture environment, cultivated mode, feeding type, living type and other comprehensive factors.
  • [1]
    农业农村部渔业渔政管理局. 2020中国渔业统计年鉴 [M]. 北京: 中国农业出版社, 2020: 26-53.
    [2]
    于佐安, 李大成, 王笑月, 等. 长海县筏养虾夷扇贝大规模死亡原因的初步研究[J]. 水产科学, 2018, 37(2): 174-180.
    [3]
    SAMAIN J F, DEGREMONT L, SOLETCHNIK P, et al. Geneti-cally based resistance to summer mortality in the Pacific oyster (Crassostrea gigas) and its relationship with physiological, immunological characteristics and infe-ctionprocesses[J]. Aquaculture, 2007, 268(1): 227-243.
    [4]
    王瑞旋, 林韵锶, 郭志勋, 等. 海南陵水企鹅珍珠贝肠道及其养殖水体中异养细菌耐药性研究[J]. 热带海洋学报, 2013, 32(6): 96-100. doi: 10.3969/j.issn.1009-5470.2013.06.014
    [5]
    农业农村部渔业渔政管理局. 中国水生动物卫生状况报告 [M]. 北京: 中国农业出版社, 2020: 3.
    [6]
    梁玉波, 张喜昌, 王立俊. 贝类典型寄生虫病害[J]. 海洋环境科学, 2003, 22(4): 72-75.
    [7]
    YOSHINAGA T, WATANABE S, WAKI T, et al. Influence of Perkinsus infection on the physiology and behavior of adult Manila clam Ruditapes philippinarum[J]. Fish Pathol, 2010, 45(4): 151-157. doi: 10.3147/jsfp.45.151
    [8]
    COOK T, FOLLI M, KLINCK J, et al. The relationship between increasing sea-surface temperature and the northward spread of Perkinsus marinus (Dermo) disease epizootics in oysters[J]. Estuar Coast Shelf S, 1998, 46(4): 587-597. doi: 10.1006/ecss.1997.0283
    [9]
    ULRICH P N, EWART J W, MARSH A G. Prevalence of Perkinsus marinus (Dermo), Haplosporidium nelsoni (MSX), and QPX in bivalves of Delaware's inland bays and quantitative, high-throughput diagnosis of dermo by qPCR[J]. J Eukaryot Microbiol, 2007, 54(6): 520-526.
    [10]
    张喜昌, 梁玉波, 杨波, 等. 海产贝类帕金虫病害的研究进展[J]. 海洋环境科学, 2001, 20(2): 76-80. doi: 10.3969/j.issn.1007-6336.2001.02.017
    [11]
    梁玉波, 张喜昌, 王立俊, 等. 北黄海菲律宾蛤仔帕金虫流行病害的研究[J]. 海洋与湖沼, 2001, 32(5): 502-511. doi: 10.3321/j.issn:0029-814X.2001.05.006
    [12]
    王江勇, 王瑞旋, 苏友禄, 等. 方斑东风螺“急性死亡症”的病原病理研究[J]. 南方水产科学, 2013, 9(5): 93-99. doi: 10.3969/j.issn.2095-0780.2013.05.015
    [13]
    黄瑜, 汪志文, 周维, 等. 徐闻方斑东风螺暴发性疾病病原分离鉴定以及防治手段探索[J]. 基因组学与应用生物学, 2016, 35(12): 3401-3409.
    [14]
    崔颖溢. 华南沿海贝类寄生帕金虫的宿主多样性、季节动态及其组织分布研究 [D]. 上海: 上海海洋大学, 2018: 22-26.
    [15]
    CUI Y Y, YE L T, WU L, et al. Seasonal occurrence of Perkinsus spp. and tissue distribution of P. olseni in clam Soletellina acuta from coastal waters of Wuchuan County, southern China[J]. Aquaculture, 2018, 492: 300-305. doi: 10.1016/j.aquaculture.2018.04.030
    [16]
    吴霖. 中国沿海贝类寄生派琴虫的分布及检测方法研究 [D]. 上海: 上海海洋大学, 2019: 1-45.
    [17]
    BUSH A O, LAFFERTY K D, LOTZ J M, et al. Parasitology meets ecology on its own terms: Margolis et al. Revisited[J]. J Parasitol, 1997, 83(4): 575-583. doi: 10.2307/3284227
    [18]
    李文祥. 黄颡鱼体内寄生蠕虫的生态学研究 [D]. 武汉: 中国科学院水生生物研究所, 2005: 1-110.
    [19]
    RAY S M. Historical perspective on Perkinsus marinus disease of oysters in the Gulf of Mexico[J]. J Shellfish Res, 1996, 15: 9-11.
    [20]
    HOLT R D, DOBSON A P, BEGON M, et al. Parasite establishment in host communities[J]. Ecol Lett, 2003, 6: 837-842. doi: 10.1046/j.1461-0248.2003.00501.x
    [21]
    TARASCHEWSKI H. Hosts and parasites as aliens[J]. J Helminthol, 2006, 80: 99-128. doi: 10.1079/JOH2006364
    [22]
    LOHAN K M P, HILL-SPANIK K M, et al. Richness and distribution of tropical oyster parasites in two oceans[J]. Parasitology, 2016, 143: 1119-1132. doi: 10.1017/S0031182015001900
    [23]
    吴霖, 叶灵通, 崔颖溢, 等. 广西沿海贝类寄生帕金虫的宿主多样性及季节动态[J]. 南方水产科学, 2018, 14(6): 113-117. doi: 10.12131/20180030
    [24]
    MOSS J A, XIAO J, DUNGAN C F, et al. Description of Perkinsus beihaiensis n. sp., a new Perkinsus sp. parasite in oysters of southern China[J]. J Eukaryot Microbiol, 2008, 55(2): 117-130. doi: 10.1111/j.1550-7408.2008.00314.x
    [25]
    CHOI K S, PARK K I I. Review on the protozoan parasite Perkinsus olseni (Lester and Davis 1981) infection in Asian waters[M]//ISHIMATSU E A, LIE H A. Coastal environmental and ecosystem issues of the East China Sea. Tokyo: Terra Scientific Publishing Company, 2010: 269-281.
    [26]
    DUNGAN C F, REECE K S. Perkinsus spp. infections of marine molluscs (2020). FHS Blue Book: suggested procedures for the detection and identification of certain finfish and shellfish pathogens[M]. Bethesda Maryland: AFS-FHS, 2020: 1-25.
    [27]
    SONIAT T M, HOFMANN E E, KLINCK J M, et al. Differential modulation of eastern oyster (Crassostrea virginica) disease parasites by the El-Niño-Southern Oscillation and the North Atlantic Oscillation[J]. Int J Earth Sci, 2009, 98(1): 99-114. doi: 10.1007/s00531-008-0364-6
    [28]
    VILLALBA A, GESTAL C, CASAS S M, et al. Perkinsosis en moluscos[M]//FIGUERAS A, NOVOA B. Enfermedades de moluscos bivalvos de interés en acuicultura. Madrid: Fundación Observatorio Español de Acuicultura, 2011: 181-242.
    [29]
    齐占会, 史荣君, 于宗赫, 等. 滤食性贝类养殖对浮游生物的影响研究进展[J]. 南方水产科学, 2021, 17(3): 115-121. doi: 10.12131/20200183
    [30]
    蔡英亚, 邓陈茂, 刘志刚. 广东鉴江尖紫蛤的生态调查[J]. 湛江水产学院学报, 1992, 12(1): 7-11.
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