Volume 15 Issue 2
Apr.  2019
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HE Peng, JIANG Shigui, LI Yundong, YANG Qibin, JIANG Song, YANG Lishi, HUANG Jianhua, ZHOU Falin. Molecular cloning and expression pattern analysis of GLUT1 in black tiger shrimp (Penaeus monodon)[J]. South China Fisheries Science, 2019, 15(2): 72-82. DOI: 10.12131/20180264
Citation: HE Peng, JIANG Shigui, LI Yundong, YANG Qibin, JIANG Song, YANG Lishi, HUANG Jianhua, ZHOU Falin. Molecular cloning and expression pattern analysis of GLUT1 in black tiger shrimp (Penaeus monodon)[J]. South China Fisheries Science, 2019, 15(2): 72-82. DOI: 10.12131/20180264
shu

Molecular cloning and expression pattern analysis of GLUT1 in black tiger shrimp (Penaeus monodon)

  • 1.

    Shenzhen Base of South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shenzhen 518121, China

  • 2.

    College of Fisheries and Life Science, Shanghai Ocean University, Shanghai 201306, China

  • 3.

    Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture and Rural Affairs; South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China

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  • Received Date: November 27, 2018
  • Revised Date: January 02, 2019
  • Accepted Date: January 27, 2019
  • Available Online: February 17, 2019
    Graphical Abstract
    • Abstract

  • We obtained the full-length cDNA sequence of GLUT1 from Penaeus monodon by rapid amplification of cDNA ends (RACE), and investigated the expression of GLUT1 at different larval developmental stages, in different tissues, and under low salinity stress by quantitative real-time PCR. The total cDNA sequence of PmGLUT1 open reading frame (ORF) was 1 476 bp, encoding 491 amino acids. From zygote to postlarva stages, the expression of PmGLUT1 fluctuated but showed an increasing trend. The PmGLUT1 was expressed in all tested tissues with the highest expression in gill tissue, followed by hepatopancreas, and the lowest expression was in ovary. After acute low salinity stress, the expression level of PmGLUT1 mRNA in hepatopancreas was significantly higher than that in the control group, while the expression level in gill was significantly lower than that in the control group (P<0.05). The results show that PmGLUT1 might play an important role at different larval developmental stages and under low salinity stress, which provides a theoretical basis for studying the molecular mechanism of PmGLUT1 in larval development and salinity adaptation of P. monodon.

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